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1. 发明授权

US09659678B2 Method for removing cesium ions from water 有权
公开(公告)号：US09659678B2
公开(公告)日：2017-05-23
申请号：US14233885
申请日：2012-07-20
申请人： Noriyuki Ohnishi , Hideyuki Hata , Xiaomao Xie
发明人： Noriyuki Ohnishi , Hideyuki Hata , Xiaomao Xie
IPC分类号： G21F9/06 , G21F9/12 , C02F1/42 , C02F1/52 , C02F1/58 , B03C1/01 , C02F1/48 , C02F101/00 , H01F1/44
CPC分类号： G21F9/06 , B03C1/01 , B03C2201/18 , C02F1/42 , C02F1/488 , C02F1/5236 , C02F2001/425 , C02F2101/006 , G21F9/12 , H01F1/44
摘要： The present invention provides a method for efficiently separating cesium ions in a short time from an aqueous solution with the number of human working steps being reduced as much as possible and recovering the cesium ions, and an apparatus therefor.Cesium ions in an aqueous solution are removed by preparing a cesium ion-containing magnetic particle in a cesium-containing aqueous solution and filtering or magnetically separating the magnetic particle.

2. 发明授权

US09297799B2 Method for measuring protozoan oocyst and detecting reagent 有权
标题翻译：测量原生动物卵囊和检测试剂的方法
公开(公告)号：US09297799B2
公开(公告)日：2016-03-29
申请号：US11813563
申请日：2006-01-11
申请人： Noriyuki Ohnishi , Hirotaka Furukawa , Hideyuki Hata , Kageaki Matsui , Takako Nogami , Hideo Nishizawa , Akihiko Kondo
发明人： Noriyuki Ohnishi , Hirotaka Furukawa , Hideyuki Hata , Kageaki Matsui , Takako Nogami , Hideo Nishizawa , Akihiko Kondo
IPC分类号： G01N33/553 , G01N33/543 , G01N33/569
CPC分类号： G01N33/54326 , G01N33/56905
摘要： The present invention provides a method for measuring oocyst of protozoa, such as Cryptosporidium, in an environment sample with high sensitivity at low cost within a short period of time; and a detecting reagent for use therein.Magnetic fine particles of 5 to 500 nm particle diameter having, immobilized thereto, binding factors for specific recognition of oocyst are added to an analyte containing a protozoan oocyst to form oocyst/binding factor/magnetic fine particle complexes by using a binding reaction to the oocyst, the formed complexes are recovered by a magnetic separation, and the protozoan oocysts contained in the complexes are assayed. Further, there is provided, for conducting the above method, a reagent for detecting protozoan oocysts comprising magnetic fine particles of 5 to 500 nm particle diameter having, immobilized thereto, antibodies against oocysts or binding factors for recognizing the antibodies.
摘要翻译：本发明提供了一种在短时间内以低成本在环境样品中测定原生动物如隐孢子虫的卵囊的方法，所述环境样品具有高灵敏度; 和其中使用的检测试剂。将固定有5〜500nm粒径的磁性细颗粒加入到含有原生动物卵囊的分析物中以形成卵囊/结合因子/磁性细颗粒复合物，通过使用与卵囊的结合反应通过磁分离回收形成的复合物，并测定复合物中所含的原生动物卵囊。此外，为了进行上述方法，提供了一种用于检测原生动物卵囊的试剂，其包括具有5至500nm粒径的磁性细颗粒，其固定有针对卵囊的抗体或用于识别抗体的结合因子。

3. 发明授权

US08815518B2 Kit for detection/quantification of analyte, and method for detection/quantification of analyte 有权
标题翻译：分析物检测/定量试剂盒，分析物检测/定量方法
公开(公告)号：US08815518B2
公开(公告)日：2014-08-26
申请号：US12306178
申请日：2007-06-28
申请人： Hirokazu Nagaoka , Noriyuki Ohnishi , Kageaki Matsui , Satoru Sugita
发明人： Hirokazu Nagaoka , Noriyuki Ohnishi , Kageaki Matsui , Satoru Sugita
IPC分类号： G01N33/53 , C12Q1/00 , G01N33/00 , B01L3/00 , G01N31/22 , G01N33/544 , G01N33/543
CPC分类号： G01N33/54313 , G01N33/54333
摘要： Disclosed are a detection/quantification kit and a detection/quantification method for detecting/quantifying an analyte rapidly at a low cost and in a simple manner. Specifically, disclosed is a kit for detecting an analyte in a sample, comprising a first conjugate and a second conjugate, wherein the first conjugate comprises a first substance comprising a stimuli-responsive polymer and a first affinity substance having affinity for the analyte and bound to the first substance, and the second conjugate comprises a second substance carrying an electrical charge and a second affinity substance having affinity for the analyte and bound to the second substance. The first and second affinity substances can bind to different sites on the analyte simultaneously.
摘要翻译：公开了一种用于以低成本和简单的方式快速检测/定量分析物的检测/定量试剂盒和检测/定量方法。具体地，公开了一种用于检测样品中的分析物的试剂盒，其包含第一缀合物和第二缀合物，其中第一缀合物包含包含刺激响应性聚合物的第一物质和对分析物具有亲和力并结合到第一物质和第二共轭物包含携带电荷的第二物质和对分析物具有亲和力并与第二物质结合的第二亲和物质。第一和第二亲和物质可以同时结合到分析物上的不同部位。

4. 发明申请

US20130164862A1 SENSING METHOD FOR BIOPOLYMERS AND SENSING DEVICE THEREFOR 有权
标题翻译：用于生物聚合物的感测方法及其感测装置
公开(公告)号：US20130164862A1
公开(公告)日：2013-06-27
申请号：US13713643
申请日：2012-12-13
申请人： Saburo TANAKA , Shinji Katsura , Noriyuki Ohnishi , Kageaki Matsui
发明人： Saburo TANAKA , Shinji Katsura , Noriyuki Ohnishi , Kageaki Matsui
IPC分类号： G01N27/72
CPC分类号： G01N33/5434
摘要： A sensing method for biopolymers by detecting the magnetic signal generated from a labeled biopolymer under AC magnetic field with thermo-responsive magnetic nano particles as label having a critical solution temperature across which the particles have the ability to aggregate or disperse with cooling or heating.
摘要翻译：通过检测在交流磁场下由标记的生物聚合物产生的磁信号的热敏磁性纳米颗粒作为具有临界溶解温度的标签的生物聚合物的感测方法，通过该临界溶液温度，颗粒具有通过冷却或加热而聚集或分散的能力。

5. 发明授权

US08105503B2 Method of storing thermoresponsive polymer in state of aqueous solution 有权
标题翻译：在水溶液状态下储存热聚合物的方法
公开(公告)号：US08105503B2
公开(公告)日：2012-01-31
申请号：US13149545
申请日：2011-05-31
申请人： Masaru Eguchi , Noriyuki Ohnishi
发明人： Masaru Eguchi , Noriyuki Ohnishi
IPC分类号： C08F20/54 , C08F6/06
CPC分类号： C09D133/26 , C08F220/58 , C08F220/60 , C08K3/24
摘要： It is intended to provide an aqueous solution of a thermoresponsive polymer, which shows an upper critical solution temperature (UCST) and is capable of maintaining a dissolved state, even if it is stored for a long period of time at a temperature at which a ligand such as an antibody or an antigen is not inactivated. A UCST lowering agent is incorporated in a predetermined amount in an aqueous solution of a thermoresponsive polymer which shows a UCST in a state of containing water, thereby lowering the UCST of the aqueous solution to less than the storage temperature, and the resulting aqueous solution is stored.
摘要翻译：旨在提供热可聚合物的水溶液，其显示上限临界溶解温度（UCST）并且能够保持溶解状态，即使其在配位体的温度下长时间储存，例如抗体或抗原未被灭活。将UCST降低剂以规定量加入到含有水的状态下显示UCST的热聚合物的水溶液中，从而将水溶液的UCST降低至低于储存温度，所得水溶液为存储。

6. 发明授权

US08105493B2 Aggregation and dispersion methods of magnetic particles, separation and detection methods using the same and detection kit 有权
标题翻译：磁性颗粒的聚集和分散方法，使用相同的分离和检测方法和检测试剂盒
公开(公告)号：US08105493B2
公开(公告)日：2012-01-31
申请号：US12147216
申请日：2008-06-26
申请人： Yuki Takahashi , Noriyuki Ohnishi , Xiaomao Xie
发明人： Yuki Takahashi , Noriyuki Ohnishi , Xiaomao Xie
IPC分类号： B03C1/01 , H01F1/00
CPC分类号： G01N33/54333 , G01N27/745 , G01N2446/84 , Y10T428/2998
摘要： It is intended to provide a method capable of simply aggregating magnetic particles having a surface modified with a thermoresponsive polymer at a given temperature without heating or cooling an aqueous solution containing the magnetic particles, and a separation method and a detection method of a substance to be detected in a sample using the method. The method of separating a substance to be detected from a sample includes the steps of: mixing an adsorbent and the sample in an aqueous solution to adsorb the substance to be detected on the adsorbent, aggregating the adsorbent by changing a salt concentration in the aqueous solution; and collecting the adsorbent from the aqueous solution by a magnetic force, wherein said adsorbent comprises a magnetic particle of an average particle size of 50 to 1000 nm, a surface of which is modified with a thermoresponsive polymer and is immobilized with a substance having an affinity for the substance to be detected.
摘要翻译：本发明的目的是提供一种能够在给定温度下简单地聚集具有用热可聚合物改性的表面的磁性颗粒而不加热或冷却含有磁性颗粒的水溶液的方法，以及物质的分离方法和检测方法使用该方法在样品中检测。从样品中分离待检测物质的方法包括以下步骤：将吸附剂和样品混合在水溶液中以将待检测物质吸附在吸附剂上，通过改变水溶液中的盐浓度来聚集吸附剂 ; 并通过磁力从水溶液中收集吸附剂，其中所述吸附剂包括平均粒径为50-1000nm的磁性颗粒，其表面用热反应性聚合物改性并用具有亲和力的物质固定用于检测物质。

7. 发明申请

US20090001321A1 AQUEOUS SOLUTION OF POLYMER HAVING UPPER CRITICAL SOLUTION TEMPERATURE, AQUEOUS DISPERSION OF PARTICLE MODIFIED WITH THE POLYMER AND METHOD OF STORING THE SAME 有权
标题翻译：具有上述关键解决方案温度的聚合物的水溶液，用聚合物改性的颗粒的水分散体及其存储方法
公开(公告)号：US20090001321A1
公开(公告)日：2009-01-01
申请号：US12147269
申请日：2008-06-26
申请人： Masaru EGUCHI , Noriyuki OHNISHI
发明人： Masaru EGUCHI , Noriyuki OHNISHI
IPC分类号： C09K3/00 , C07K2/00
CPC分类号： C09D133/26 , C08F220/58 , C08F220/60 , C08K3/24
摘要： It is intended to provide an aqueous solution of a thermoresponsive polymer and an aqueous dispersion of a thermoresponsive particle, which show an upper critical solution temperature (UCST) and are capable of maintaining a dissolved state and a dispersed state, respectively, even if they are stored for a long period of time at a temperature at which a ligand such as an antibody or an antigen is not inactivated. A UCST lowering agent is incorporated in a predetermined amount in an aqueous solution of a thermoresponsive polymer which shows a UCST in a state of containing water or an aqueous dispersion of a thermoresponsive particle containing the polymer, thereby lowering the UCST of the aqueous solution or aqueous dispersion to less than the storage temperature, and the resulting aqueous solution or aqueous dispersion is stored.
摘要翻译：旨在提供热敏聚合物的水溶液和热反应性颗粒的水分散体，其显示出上限临界溶解温度（UCST），并且能够分别保持溶解状态和分散状态，即使它们是在抗体或抗原等配体未失活的温度下长时间贮存。将UCST降低剂以预定量加入到表现出包含水的UCST或包含聚合物的热敏颗粒的水分散体的热稳定聚合物的水溶液中，从而降低水溶液的UCST或水溶液分散至低于储存温度，并将所得水溶液或水分散体储存。

8. 发明申请

US20080199884A1 Method For Measuring Protozoan Oocyst and Detecting Reagent 有权
标题翻译：测定原生动物卵囊和检测试剂的方法
公开(公告)号：US20080199884A1
公开(公告)日：2008-08-21
申请号：US11813563
申请日：2006-01-11
申请人： Noriyuki Ohnishi , Hirotaka Furukawa , Hideyuki Hata , Kageaki Matsui , Takako Nogami , Hideo Nishizawa , Akihiko Kondo
发明人： Noriyuki Ohnishi , Hirotaka Furukawa , Hideyuki Hata , Kageaki Matsui , Takako Nogami , Hideo Nishizawa , Akihiko Kondo
IPC分类号： G01N33/53
CPC分类号： G01N33/54326 , G01N33/56905
摘要： The present invention provides a method for measuring oocyst of protozoa, such as Cryptosporidium, in an environment sample with high sensitivity at low cost within a short period of time; and a detecting reagent for use therein.Magnetic fine particles of 5 to 500 nm particle diameter having, immobilized thereto, binding factors for specific recognition of oocyst are added to an analyte containing a protozoan oocyst to form oocyst/binding factor/magnetic fine particle complexes by using a binding reaction to the oocyst, the formed complexes are recovered by a magnetic separation, and the protozoan oocysts contained in the complexes are assayed. Further, there is provided, for conducting the above method, a reagent for detecting protozoan oocysts comprising magnetic fine particles of 5 to 500 nm particle diameter having, immobilized thereto, antibodies against oocysts or binding factors for recognizing the antibodies.
摘要翻译：本发明提供了一种在短时间内以低成本在环境样品中测定原生动物如隐孢子虫的卵囊的方法，所述环境样品具有高灵敏度; 和其中使用的检测试剂。将固定有5〜500nm粒径的磁性细颗粒加入到含有原生动物卵囊的分析物中以形成卵囊/结合因子/磁性细颗粒复合物，通过使用与卵囊的结合反应通过磁分离回收形成的复合物，并测定复合物中所含的原生动物卵囊。此外，为了进行上述方法，提供了一种用于检测原生动物卵囊的试剂，其包括具有5至500nm粒径的磁性细颗粒，其固定有针对卵囊的抗体或用于识别抗体的结合因子。

9. 发明申请

US20050158782A1 Magnetic fine particles having lower critical solution temperature 审中-公开
标题翻译：具有较低临界溶解温度的磁性细颗粒
公开(公告)号：US20050158782A1
公开(公告)日：2005-07-21
申请号：US11030149
申请日：2005-01-07
申请人： Hirotaka Furukawa , Noriyuki Ohnishi , Kazunori Kataoka , Katsuhiko Ueno
发明人： Hirotaka Furukawa , Noriyuki Ohnishi , Kazunori Kataoka , Katsuhiko Ueno
IPC分类号： C07D495/04 , C07K17/14 , C12N11/14 , C12N15/10 , G01N33/543 , C12Q1/68
CPC分类号： C07D495/04 , C07K17/14 , C12N11/14 , C12N15/1013 , G01N33/5434 , G01N2333/255 , G01N2446/86
摘要： The present invention relates to magnetic fine particles having a lower critical solution temperature to which at least one substance selected from biotin and avidin is immobilized, and a method of converting a substance, a method of separating or concentrating a microorganism, a method of modifying a denatured protein, a method of detecting a nucleic acid, a separating agent, and a method of separating a biological substance using the same.
摘要翻译：本发明涉及固定有至少一种选自生物素和抗生物素蛋白的物质的临界溶解温度较低的磁性微粒，以及将物质转化的方法，微生物的分离或浓缩方法，改性方法变性蛋白质，检测核酸的方法，分离剂以及使用其分离生物物质的方法。

10. 发明授权

US06863437B2 Temperature-responsive polymer/polymer complex 有权
标题翻译：温度敏感聚合物/聚合物复合物
公开(公告)号：US06863437B2
公开(公告)日：2005-03-08
申请号：US10362167
申请日：2001-08-20
申请人： Noriyuki Ohnishi , Hirotaka Furukawa , Kazunori Kataoka , Katsuhiko Ueno
发明人： Noriyuki Ohnishi , Hirotaka Furukawa , Kazunori Kataoka , Katsuhiko Ueno
IPC分类号： C08L29/04 , C08L33/26 , C08L71/02 , C12N11/08 , G01N25/20 , G01K1/00 , G01K11/16
CPC分类号： C12N11/08 , C08L29/04 , C08L33/26 , C08L71/02 , Y10S430/165 , C08L2666/14 , C08L2666/04 , C08L2666/02
摘要： The problem of the invention is to find a polymer mixture which forms an inter-polymer complex by being responsive to temperature even under neutral to alkaline conditions. According to the invention, a temperature responsive inter-polymer complex capable of showing thermal responsiveness in an aqueous solution, which contains a poly-N-acetylacrylamide or polyvinyl alcohol and polyethylene glycol, polyacrylamide or polymethacylamide, is applied to separating agents, immobilized enzymes, denatured protein modifiers, separation method or concentration of microorganisms, purification or concentration of nucleic acids, drug-releasing microcapsules and the like.
摘要翻译：本发明的问题是找到一种通过即使在中性至碱性条件下也适应于温度形成聚合物间络合物的聚合物混合物。根据本发明，能够在包含聚-N-乙酰丙烯酰胺或聚乙烯醇和聚乙二醇，聚丙烯酰胺或聚甲基酰胺的水溶液中显示热响应性的温度敏感性聚合物络合物被施加到分离剂，固定化酶，变性蛋白质改性剂，分离方法或微生物浓度，核酸的纯化或浓缩，药物释放微胶囊等。

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