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    • 2. 发明授权
    • Conformational probes and methods for sequencing nucleic acids
    • 构象探针和核酸测序方法
    • US09353412B2
    • 2016-05-31
    • US13162325
    • 2011-06-16
    • Molly HeCheng-Yao ChenEric KoolMostafa RonaghiMichael PreviteRigo Pantoja
    • Molly HeCheng-Yao ChenEric KoolMostafa RonaghiMichael PreviteRigo Pantoja
    • C12P19/34C12Q1/68
    • C12Q1/6874C12Q1/6806C12Q1/6818C12Q2565/133C12Q2563/125C12Q2525/101C12Q2565/1015
    • This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.
    • 本公开提供了确定核酸模板的核苷酸序列的方法。 该方法可以包括以下步骤:使构象标记的聚合酶和至少四种不同的核苷酸物种在其中所述构象标记的聚合酶催化核苷酸物种的顺序加入以形成核酸模板的核酸补体的条件下接触, 其中每个不同核苷酸类型的顺序加入从所述构象标记的聚合酶产生构象信号变化,并且其中所述构象信号变化的速率或持续时间对于每个不同的核苷酸种类是可区分的; 在条件下检测来自构象标记的聚合酶的信号的一系列变化; 并确定信号变化的速率或时间,从而确定核酸模板的核苷酸序列。