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1. 发明授权

US08394944B2 Dual-purpose primers and probes for providing enhanced hybridization assays by disruption of secondary structure formation 有权
标题翻译：双用途引物和探针，通过破坏二级结构形成提供增强的杂交测定
公开(公告)号：US08394944B2
公开(公告)日：2013-03-12
申请号：US10667191
申请日：2003-09-15
申请人： Minxue Zheng , John J. Quinn , Brian D. Warner
发明人： Minxue Zheng , John J. Quinn , Brian D. Warner
IPC分类号： C07H21/04 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6858 , C12Q1/6827 , C12Q1/6832 , C12Q1/686 , C12Q2565/519 , C12Q2525/301 , C12Q2525/197 , C12Q2525/186 , C12Q2525/313
摘要： The present invention provides primers and probes to be used in a method of enhancing hybridization of a probe to a target nucleotide sequence when the target sequence is capable of forming intramolecular secondary structures that interfere with hybridization of the probe to the target sequence. In particular, the invention includes a primer for amplifying a target nucleotide sequence, wherein at least a portion of the target nucleotide sequence can form an intramolecular secondary structure. The primer of the invention includes a primer nucleotide sequence complementary to a portion of the target nucleotide sequence that does not form a secondary structure, and a blocking sequence substantially complementary to at least a portion of the secondary structure-forming region of the amplified target nucleotide sequence, wherein the blocking sequence hybridizes to a portion of the secondary structure-forming region of the amplified target nucleotide sequence and blocks the formation of the secondary structure.
摘要翻译：本发明提供了当靶序列能够形成干扰探针与靶序列的杂交的分子内二级结构时，用于增强探针与靶核苷酸序列的杂交的方法中的引物和探针。特别地，本发明包括用于扩增靶核苷酸序列的引物，其中目标核苷酸序列的至少一部分可以形成分子内二级结构。本发明的引物包括与未形成二级结构的靶核苷酸序列的一部分互补的引物核苷酸序列和与扩增的靶核苷酸的至少一部分二级结构形成区基本上互补的阻断序列序列，其中所述阻断序列与扩增的靶核苷酸序列的二级结构形成区的一部分杂交并阻断二级结构的形成。

2. 发明授权

US08063196B2 Highly orthogonal universal sequences for use in nucleic acid assays 有权
标题翻译：用于核酸测定的高度正交通用序列
公开(公告)号：US08063196B2
公开(公告)日：2011-11-22
申请号：US11049465
申请日：2005-02-01
申请人： Minxue Zheng , David Ahle , Brian Warner , Ming Wu , Chu-an Chang
发明人： Minxue Zheng , David Ahle , Brian Warner , Ming Wu , Chu-an Chang
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/682 , Y02A50/54 , C12Q2525/101
摘要： The invention provides a set of highly orthogonal six-code universal sequences for use in bDNA singleplex and multiplex nucleic acid hybridization assays. The six-code orthogonal sequences do not cross-hybridize and thus, minimize or eliminate the 3-mer cross-hybridization inherent in the second and third generation bDNA assays. The highly orthogonal universal sequences may be used in singleplex or multiplex bDNA assays quantitatively and qualitatively to determine mRNA levels in a sample; to screen for and genotype targets, such as viruses, that are present in low volumes in a sample; to screen for and genotype SNPs; and to measure changes in the amount of a gene in a sample such as when gene amplifications or deletions occur. The highly orthogonal universal sequences may also be used as universal capture probes to selectively bind assay components in a way that facilitates their further analysis.
摘要翻译：本发明提供了一组高度正交的六码通用序列，用于bDNA单链和多重核酸杂交测定。六码正交序列不交叉杂交，因此最小化或消除第二代和第三代bDNA测定中固有的3聚体交叉杂交。高度正交的通用序列可以定量和定性地用于单链或多重bDNA测定以确定样品中的mRNA水平; 筛选和基因型样品中低体积存在的目标，如病毒; 筛选和基因型SNP; 并且测量样品中基因的量的变化，例如当发生基因扩增或缺失时。高度正交的通用序列还可以用作通用捕获探针，以便于进一步分析的方式选择性地结合测定组分。

3. 发明申请

US20100047800A1 Reagents and Methods for Detecting CYP2C9 Polymorphisms 审中-公开
标题翻译：检测CYP2C9多态性的试剂和方法
公开(公告)号：US20100047800A1
公开(公告)日：2010-02-25
申请号：US12523000
申请日：2008-01-22
申请人： Minxue Zheng , Lailing Ku , Lu-ping Shen , Carrie Wong , Charlene Bush-Donovan
发明人： Minxue Zheng , Lailing Ku , Lu-ping Shen , Carrie Wong , Charlene Bush-Donovan
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6883 , C12Q2600/106 , C12Q2600/156 , C12Q2600/16
摘要： The present invention relates to oligonucleotide sequences for amplification primers and detection probes and their use in nucleic acid amplification methods for the specific detection of clinically relevant CYP2C9 polymorphisms, in particular CYP2C9 polymorphisms associated with adverse drug response. The oligonucleotide sequences are also provided assembled as kits that can be used to predict how an individual will respond to drugs or other xenobiotic compounds that are metabolized, at least in part, by CYP2C9.
摘要翻译：本发明涉及用于扩增引物和检测探针的寡核苷酸序列及其在核酸扩增方法中用于特异性检测临床相关CYP2C9多态性的用途，特别是与不良药物反应相关的CYP2C9多态性。还提供寡核苷酸序列组装成试剂盒，其可用于预测个体如何对至少部分由CYP2C9代谢的药物或其它异生素化合物作出反应。

4. 发明申请

US20060172284A1 Highly orthogonal universal sequences for use in nucleic acid assays 有权
标题翻译：用于核酸测定的高度正交通用序列
公开(公告)号：US20060172284A1
公开(公告)日：2006-08-03
申请号：US11049465
申请日：2005-02-01
申请人： Minxue Zheng , David Ahle , Brian Warner , Ming Wu , Chu-an Chang
发明人： Minxue Zheng , David Ahle , Brian Warner , Ming Wu , Chu-an Chang
IPC分类号： C12Q1/70 , C12Q1/68 , C07H21/02 , C12P19/34
CPC分类号： C12Q1/682 , Y02A50/54 , C12Q2525/101
摘要： The invention provides a set of highly orthogonal six-code universal sequences for use in bDNA singleplex and multiplex nucleic acid hybridization assays. The six-code orthogonal sequences do not cross-hybridize and thus, minimize or eliminate the 3-mer cross-hybridization inherent in the second and third generation bDNA assays. The highly orthogonal universal sequences may be used in singleplex or multiplex bDNA assays quantitatively and qualitatively to determine mRNA levels in a sample; to screen for and genotype targets, such as viruses, that are present in low volumes in a sample; to screen for and genotype SNPs; and to measure changes in the amount of a gene in a sample such as when gene amplifications or deletions occur. The highly orthogonal universal sequences may also be used as universal capture probes to selectively bind assay components in a way that facilitates their further analysis.
摘要翻译：本发明提供了一组高度正交的六码通用序列，用于bDNA单链和多重核酸杂交测定。六码正交序列不交叉杂交，因此最小化或消除第二代和第三代bDNA测定中固有的3聚体交叉杂交。高度正交的通用序列可以定量和定性地用于单链或多重bDNA测定以确定样品中的mRNA水平; 筛选和基因型样品中低体积存在的目标，如病毒; 筛选和基因型SNP; 并且测量样品中基因的量的变化，例如当发生基因扩增或缺失时。高度正交的通用序列还可以用作通用捕获探针，以便于进一步分析的方式选择性地结合测定组分。

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