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1. 发明授权

US4294817A Method of fluoro immunoassay 失效
标题翻译：氟免疫测定法
公开(公告)号：US4294817A
公开(公告)日：1981-10-13
申请号：US943314
申请日：1978-09-18
申请人： Michael W. Burgett , Richard A. Harte
发明人： Michael W. Burgett , Richard A. Harte
IPC分类号： G01N33/533 , G01N33/543 , G01N33/58 , G01N1/28 , G01N21/25 , G01N33/54
CPC分类号： G01N33/543 , Y10S435/962
摘要： A new surface immunoassay method is performed with two test surfaces. The method is used where serum samples may contain both an antibody for which it is desirable to test, and also competing sera constituents which may be present in indeterminate amounts. In accordance with the method, two different test surfaces are contacted with the same serum sample, and preferably the same aliquot of the serum. The first surface is treated to bind a broad spectrum of serum components including both the component to be tested for and also the competing components. The second surface is designed to bind the competing components but no substantial amount of the serum component to be tested for. The immunoassay is conducted with a labeled antibody, and quantitative measurement of the immunoassay is obtained by detecting the quantitative difference in the amount of labeled antibody on the two surfaces which have been subjected to the same serum sample.The method has utility in detecting a wide variety of antibodies of both the immunoglobulin G and immunoglobulin M classes, including antibodies for rubella, treponema, herpes simplex virus, cytomegalovirus and toxoplasma.Preferably the two surfaces used in the test are mounted on a single sampler which is analyzed in the FIAX.RTM. Fluorometer.
摘要翻译：用两个测试表面进行新的表面免疫测定方法。使用该方法，其中血清样品可以含有期望测试的抗体，并且还可以以不确定的量存在的竞争血清成分。根据该方法，将两个不同的测试表面与相同的血清样品接触，优选与血清相同的等分试样接触。处理第一表面以结合广谱的血清成分，包括要测试的成分和竞争成分。第二表面被设计成结合竞争组分，但没有大量的待测试的血清组分。用标记的抗体进行免疫测定，通过检测已经经过相同血清样品的两个表面上的标记抗体的量的定量差异来获得免疫测定的定量测量。该方法可用于检测免疫球蛋白G和免疫球蛋白M类的各种抗体，包括风疹，螺旋体，单纯疱疹病毒，巨细胞病毒和弓形体的抗体。优选地，在测试中使用的两个表面安装在单个采样器上，其在FIAX TM荧光计中进行分析。

2. 发明授权

US4088746A Radioimmunoassay for thyroid-stimulating hormone (TSH) 失效
标题翻译：甲状腺刺激激素（TSH）的放射免疫测定
公开(公告)号：US4088746A
公开(公告)日：1978-05-09
申请号：US742391
申请日：1976-11-11
申请人： Judith I. Blakemore , Nathan Lewin , Michael W. Burgett
发明人： Judith I. Blakemore , Nathan Lewin , Michael W. Burgett
IPC分类号： G01N33/53 , G01N33/78 , A61K43/00 , G01N33/16
CPC分类号： G01N33/78 , Y10S436/804
摘要： This invention provides a method for the radioimmunoassay of thyroid-stimulating hormone which utilizes a rapid and convenient version of a double antibody procedure. Highly purified second antibody is bound, by means of covalent bonds, to hydrolyzed polyacrylamide particles to produce a two-phase system. The solid phase comprises immobilized second antibody bound to the reaction product of labeled and unlabeled thyroid-stimulating hormone with the first antibody (first antibody-antigen complex) and the liquid phase comprises free (unbound) labeled and unlabeled thyroid-stimulating hormone. The two phases are separated and the radioactivity of either phase is measured.
摘要翻译：本发明提供一种利用双抗体程序的快速方便版本的促甲状腺激素的放射免疫测定方法。通过共价键将高度纯化的第二抗体结合到水解的聚丙烯酰胺颗粒上以产生两相体系。固相包含与标记和未标记的促甲状腺激素与第一抗体（第一抗体 - 抗原复合物）的反应产物结合的固定的第二抗体，液相包含游离（未结合的）标记和未标记的促甲状腺激素。两相分离，测定两相的放射性。

3. 发明授权

US4254096A Reagent combination for solid phase immunofluorescent assay 失效
标题翻译：用于固相免疫荧光测定的试剂组合
公开(公告)号：US4254096A
公开(公告)日：1981-03-03
申请号：US81741
申请日：1979-10-04
申请人： James F. Monthony , Michael W. Burgett , Robert V. Dahlstrom
发明人： James F. Monthony , Michael W. Burgett , Robert V. Dahlstrom
IPC分类号： G01N33/543 , G01N33/52 , G01N33/54
CPC分类号： G01N33/54313 , Y10S436/80
摘要： An assay method suitable for antigens (or haptens) including proteins and polypeptides utilizing antibodies for the unknown protein or polypeptide in which the antibodies are covalently bound to water insoluble hydrophilic polymeric particles. Appropriate fluorescently labeled immune reactants are introduced during the method and together with the unknown antigen are immunologically bound, directly or indirectly, to the particles for separation from the reaction liquid. The particles have a size of about 0.1-10 microns and can be resuspended for direct measurement of the fluorescent labels in a fluorometer from which the unknown can be determined.
摘要翻译：适用于包含蛋白质和多肽的抗原（或半抗原）的测定方法，其利用抗体共价结合水不溶性亲水性聚合物颗粒的未知蛋白质或多肽的抗体。在该方法期间引入适当的荧光标记的免疫反应物，并且与未知抗原一起直接或间接地与颗粒免疫结合以分离反应液体。颗粒具有约0.1-10微米的尺寸，并且可以重新悬浮以便在荧光计中直接测量荧光标记，从中可以确定未知物。

4. 发明授权

US4201763A Solid phase immunofluorescent assay method 失效
标题翻译：固相免疫荧光测定法
公开(公告)号：US4201763A
公开(公告)日：1980-05-06
申请号：US875475
申请日：1978-02-06
申请人： James F. Monthony , Michael W. Burgett , Robert V. Dahlstrom
发明人： James F. Monthony , Michael W. Burgett , Robert V. Dahlstrom
IPC分类号： G01N33/543 , G01N21/00 , G01N21/38 , G01N31/22 , G01N33/16
CPC分类号： G01N33/54313 , Y10S436/80
摘要： An assay method suitable for antigens (or haptens) including proteins and polypeptides utilizing antibodies for the unknown protein or polypeptide in which the antibodies are covalently bound to water insoluble hydrophylic polymeric particles. Appropriate fluorescently labeled immune reactants are introduced during the method and together with the unknown antigen are immunologically bound, directly or indirectly, to the particles for separation from the reaction liquid. The particles have a size of about 0.1-10 microns and can be resuspended for direct measurement of the fluorescent labels in a fluorometer from which the unknown can be determined.
摘要翻译：适用于包含蛋白质和多肽的抗原（或半抗原）的测定方法，其利用抗体共价结合水不溶性亲水聚合物颗粒的未知蛋白质或多肽的抗体。在该方法期间引入适当的荧光标记的免疫反应物，并且与未知抗原一起直接或间接地与颗粒免疫结合以分离反应液体。颗粒具有约0.1-10微米的尺寸，并且可以重新悬浮以便在荧光计中直接测量荧光标记，从中可以确定未知物。

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