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1. 发明申请

US20100167262A1 METHOD AND REAGENT FOR THE SPECIFIC IDENTIFICATION AND QUANTIFICATION OF ONE OR MORE PROTEINS IN A SAMPLE USING IN PARTICULAR INDUCTIVELY COUPLED PLASMA-MASS SPECTROMETRY 审中-公开
标题翻译：用于特定感应耦合等离子体光谱法中样品中一种或多种蛋白质的特异性鉴定和定量的方法和试剂
公开(公告)号：US20100167262A1
公开(公告)日：2010-07-01
申请号：US12297092
申请日：2007-04-13
申请人： Michael Linscheid , Stefan Pieper , Christian Scheler , Robert Ahrends
发明人： Michael Linscheid , Stefan Pieper , Christian Scheler , Robert Ahrends
IPC分类号： C12Q1/00 , G01N33/00
CPC分类号： G01N33/6848
摘要： The invention relates to a method for the identification and quantification of one or more biomolecules in a sample containing a mixture of substances, wherein said method comprises the steps of, a) providing a sample which contains one or more biomolecules, b) providing a reagent for the analysis of biomolecules according to Formula I (A-Y-PRG) wherein, A may be present or absent and when A is present, A is H or constitutes at least one functional group for the reversible, covalent or non-covalent binding to a support material, whereby Y is a group comprising at least one chelate function for metals, preferable low in isotopes, and whereby PRG is a reactive group for the selective binding of biomolecules to be analyzed, c) coupling the one or more biomolecules from step a) to the reagent of step b), d) selecting the one or more biomolecules labeled in step c) under the employment of a functional group for the reversible, covalent or non-covalent binding to a support material and removal of the one or more unbound biomolecules, wherein (d) optionally comprises releasing the bound biomolecules from the support material and the elution from the matrix; and f) detecting and identifying the one or more labeled biomolecules by means of Inductively Coupled Plasma-Mass Spectrometry (ICP-MS), wherein f) optionally comprises cleaving the one or more biomolecules in said sample wherein, said cleaving may be performed (i) before coupling of the reagent PRG or (ii) after coupling.
摘要翻译：本发明涉及用于鉴定和定量含有物质混合物的样品中一种或多种生物分子的方法，其中所述方法包括以下步骤：a）提供含有一种或多种生物分子的样品，b）提供试剂用于分析根据式I的生物分子（AY-PRG），其中A可以存在或不存在，并且当存在A时，A是H或构成至少一个用于可逆，共价或非共价结合到一个支撑材料，其中Y是包含金属的至少一种螯合官能团，优选低同位素的组，并且由此PRG是用于选择性结合待分析的生物分子的反应性基团，c）将来自步骤a的一种或多种生物分子）到步骤b）的试剂中，d）在使用官能团的情况下选择步骤c）中标记的一种或多种生物分子，以进行可逆，共价或非共价结合到载体材料和去除所述一个或多个未结合的生物分子，其中（d）任选地包括从所述载体材料中释放结合的生物分子并从所述基质中洗脱; 和f）通过电感耦合等离子体质谱法（ICP-MS）检测和识别所述一种或多种标记的生物分子，其中f）任选地包括切割所述样品中的一种或多种生物分子，其中所述切割可以进行（i ）或（ii）偶联之后。

2. 发明申请

US20060246530A1 Method and reagent for the specifically identifying and quantifying one or more proteins in a sample 审中-公开
标题翻译：用于特异性鉴定和定量样品中一种或多种蛋白质的方法和试剂
公开(公告)号：US20060246530A1
公开(公告)日：2006-11-02
申请号：US10518727
申请日：2003-06-13
申请人： Martin Krause , Christian Scheler , Ulrike Bottger , Hardy Weisshoff , Michael Linscheid
发明人： Martin Krause , Christian Scheler , Ulrike Bottger , Hardy Weisshoff , Michael Linscheid
IPC分类号： C12Q1/37 , G01N33/00 , C07K14/47
CPC分类号： G01N33/6848
摘要： The present invention relates to the MeCAT (Metal-chelate-complex-coded-affinity-tag)-method and to a reagent suitable for performing said method, which method includes a reproducible, systematic, qualitative and quantitative proteome characterization by means of non-isotope metal coded markers and—among other items—the most modem tandem methods of mass spectrometry.
摘要翻译：本发明涉及MeCAT（金属螯合物 - 复合物编码亲和标记）方法和适用于进行所述方法的试剂，该方法包括可重复的，系统的，定性的和定量的蛋白质组学表征，同位素金属编码标记和其他项目 - 最现代的串联质谱法。

3. 发明申请

US20060246225A1 Sample carrier based on a porous film with metal oxide particles, the production and utilization thereof, especially for selective detection of phosphorylated/sulphatized biopolymers 审中-公开
标题翻译：基于具有金属氧化物颗粒的多孔膜的样品载体，其生产和利用，特别是用于选择性检测磷酸化/硫化生物聚合物
公开(公告)号：US20060246225A1
公开(公告)日：2006-11-02
申请号：US10556485
申请日：2004-05-14
申请人： Thomas Moritz , Michael Linscheid
发明人： Thomas Moritz , Michael Linscheid
IPC分类号： B05D3/02
CPC分类号： H01J49/0418 , G01N33/6842 , G01N33/6848
摘要： The present invention relates to a porous film comprising metal oxide particles, to a sample holder using the film, to a method of preparation for the sample holder, to the application of the sample holder as well as to a method of detecting phosphorylated/sulphated biopolymers, specifically peptides/proteins.
摘要翻译：本发明涉及包含金属氧化物颗粒的多孔膜，使用该膜的样品保持器，样品保持器的制备方法，样品保持器的应用以及检测磷酸化/硫酸化生物聚合物的方法，特别是肽/蛋白质。

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