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1. 发明授权

US5232664A Liquid dispenser 失效
标题翻译：液体分配器
公开(公告)号：US5232664A
公开(公告)日：1993-08-03
申请号：US762327
申请日：1991-09-18
申请人： Thomas P. Krawzak , Michael J. DeGroff , David W. Nunnery , Brian J. McGraw
发明人： Thomas P. Krawzak , Michael J. DeGroff , David W. Nunnery , Brian J. McGraw
IPC分类号： G01N1/00 , G01N1/14 , G01N35/00 , G01N35/04 , G01N35/10
CPC分类号： G01N35/1002 , G01N2035/0443 , G01N2035/102 , G01N2035/1023 , Y10T436/119163 , Y10T436/2575
摘要： A dispenser which can deliver precise small amounts of a liquid is disclosed. The dispenser is particularly suited to applying reagents in a biological test such as an immunoassay. It includes a reservoir chamber linked to a dispense chamber via a check valve, the two chambers preferably being in the form of syringes. An outlet line leading from the dispense chamber to a nozzle includes a delay check valve (preferably a ball inside a cylindrical chamber) which allows continuous outward flow, but shuts off flow in the reverse direction after allowing a predetermined volume to pass. The process of refilling the dispense chamber from the reservoir chamber creates reverse flow in the nozzle and causes a hanging drop of liquid at the tip of the nozzle to be withdrawn.
摘要翻译：公开了一种能够输送精确少量液体的分配器。分配器特别适用于在诸如免疫测定的生物测试中施用试剂。其包括通过止回阀连接到分配室的储存室，两个室优选地为注射器的形式。从分配器引导到喷嘴的出口管线包括允许连续向外流动的延迟止回阀（优选地是圆柱形室内的球），但在允许预定体积通过之后，反向流动。从分配室向分注室重新注入的过程在喷嘴中产生反向流动并导致在喷嘴尖端处的悬滴液体被抽出。

2. 发明授权

US5225325A Immunohistochemical staining method and reagents therefor 失效
标题翻译：免疫组织化学染色方法和试剂
公开(公告)号：US5225325A
公开(公告)日：1993-07-06
申请号：US488348
申请日：1990-03-02
申请人： Phillip C. Miller , Michael J. Degroff , Michael J. Gizinski , James A. Rybski , Pamela S. Vandivort
发明人： Phillip C. Miller , Michael J. Degroff , Michael J. Gizinski , James A. Rybski , Pamela S. Vandivort
IPC分类号： C12N9/96 , C12Q1/28 , G01N1/30 , G01N33/48 , G01N33/53 , G01N33/531 , G01N33/535 , G01N33/543 , G01N33/567 , G01N33/58 , G01N35/00
CPC分类号： G01N33/535 , C12N9/96 , C12Q1/28 , G01N1/30 , G01N33/54393 , G01N33/567 , G01N33/581 , C12Q2326/10 , G01N2035/00079 , G01N2035/00277 , Y10S435/96 , Y10S435/961 , Y10S435/962 , Y10S435/963 , Y10S436/825 , Y10T436/112499 , Y10T436/25 , Y10T436/25125
摘要： The present invention provides an immunohistochemical staining method using improved reagents. In one aspect, the method uses an evaporation inhibitor liquid to prevent evaporation of reagents applied to an assay region of a slide.
摘要翻译：本发明提供使用改良试剂的免疫组织化学染色方法。在一个方面，该方法使用蒸发抑制剂液体来防止施加到载玻片的测定区域的试剂的蒸发。

3. 发明授权

US5418138A Immunohistochemical staining method and reagents therefor 失效
标题翻译：免疫组织化学染色法及其试剂
公开(公告)号：US5418138A
公开(公告)日：1995-05-23
申请号：US212415
申请日：1994-03-11
申请人： Phillip C. Miller , Michael J. Degroff , Michael J. Gizinski , James A. Rybski , Pamela S. Vandivort , Anthony L. Hartman
发明人： Phillip C. Miller , Michael J. Degroff , Michael J. Gizinski , James A. Rybski , Pamela S. Vandivort , Anthony L. Hartman
IPC分类号： C12N9/96 , C12Q1/28 , G01N1/30 , G01N33/48 , G01N33/53 , G01N33/531 , G01N33/535 , G01N33/543 , G01N33/567 , G01N33/58 , G01N35/00
CPC分类号： G01N33/535 , C12N9/96 , C12Q1/28 , G01N1/30 , G01N33/54393 , G01N33/567 , G01N33/581 , C12Q2326/10 , G01N2035/00079 , G01N2035/00277 , Y10S435/96 , Y10S435/961 , Y10S435/962 , Y10S435/963 , Y10S436/825 , Y10T436/112499 , Y10T436/25 , Y10T436/25125
摘要： The present invention provides an improved method for staining slides using immunochemical reagents. The method comprises the following steps. The assay region of a slide (the region containing the tissue section)is washed with an improved rinsing solution comprising water and a detergent. An evaporation inhibitor liquid is applied to the slide to cover the assay region. For antigens requiring unmasking, the tissue section is combined with an improved, stabilized proteolytic enzyme solution. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. A primary antibody in an improved diluent containing globulins from the same species as a second antibody is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied. A labeled second antibody in the improved diluent is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. Color development reagents, including a stabilized peroxidase chromophore formulation, are combined with the tissue section for a time sufficient for color development. The stabilized peroxidase chromophore formulation consists essentially of a peroxidase chromophore, at a concentration in the working range of the enzyme, an acidic buffer, a reducing agent, and a glycol. Preferred chromophores include 3,3'-diaminobenzidine and tetrahydrochloride (DAB) and 3-amino-9-ethylcarbazole (AEC). After color development, the tissue section is washed and is then ready for analysis.
摘要翻译：本发明提供了使用免疫化学试剂对载玻片进行染色的改进方法。该方法包括以下步骤。载玻片的测定区域（含有组织切片的区域）用包含水和洗涤剂的改进的冲洗溶液洗涤。将蒸发抑制剂液体施加到载玻片上以覆盖测定区域。对于需要揭发的抗原，将组织切片与改良的稳定的蛋白水解酶溶液组合。将载玻片漂洗，并将蒸发抑制剂液体重新施加到载玻片上。将来自与第二抗体相同物种的球蛋白的改进的稀释剂中的一抗与组织切片组合足够的时间，足以基本上完全抗体结合。将载玻片漂洗，并重新施加蒸发抑制剂液体。将改进的稀释剂中的标记的第二抗体与组织切片组合足够长的时间以使基本上完全的抗体结合。将载玻片漂洗，并将蒸发抑制剂液体重新施加到载玻片上。包括稳定的过氧化物酶发色团配方的显色试剂与组织切片组合足够的时间用于显色。稳定的过氧化物酶发色团配方主要由过氧化物酶发色团组成，浓度在酶的工作范围内，酸性缓冲液，还原剂和二醇。优选的发色团包括3,3'-二氨基联苯胺和四盐酸盐（DAB）和3-氨基-9-乙基咔唑（AEC）。在显色后，洗涤组织切片，然后准备分析。

4. 发明授权

US5322771A Immunohistochemical staining method and reagents therefor 失效
标题翻译：免疫组织化学染色法及其试剂
公开(公告)号：US5322771A
公开(公告)日：1994-06-21
申请号：US924053
申请日：1992-08-31
申请人： James A. Rybski , Pamela S. Vandivort , Anthony L. Hartman , Phillip C. Miller , Michael J. Degroff , Michael J. Gizinski
发明人： James A. Rybski , Pamela S. Vandivort , Anthony L. Hartman , Phillip C. Miller , Michael J. Degroff , Michael J. Gizinski
IPC分类号： C12N9/96 , C12Q1/28 , G01N1/30 , G01N33/48 , G01N33/53 , G01N33/531 , G01N33/535 , G01N33/543 , G01N33/567 , G01N33/58 , G01N35/00 , C12Q1/37 , G01N1/34
CPC分类号： G01N33/535 , C12N9/96 , C12Q1/28 , G01N1/30 , G01N33/54393 , G01N33/567 , G01N33/581 , C12Q2326/10 , G01N2035/00079 , G01N2035/00277 , Y10S435/96 , Y10S435/961 , Y10S435/962 , Y10S435/963 , Y10S436/825 , Y10T436/112499 , Y10T436/25 , Y10T436/25125
摘要： The present invention provides an improved method for staining slides using immunochemical reagents. The method comprises the following steps. The assay region of a slide (the region containing the tissue section) is washed with an improved rinsing solution comprising water and a detergent. An evaporation inhibitor liquid is applied to the slide to cover the assay region. For antigens requiring unmasking, the tissue section is combined with an improved, stabilized proteolytic enzyme solution. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. A primary antibody in an improved diluent containing globulins from the same species as a second antibody is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied. A labeled second antibody in the improved diluent is combined with the tissue section for a time sufficient for substantially complete antibody binding. The slide is rinsed, and the evaporation inhibitor liquid is reapplied to the slide. Color development reagents, including, in a preferred embodiment, a stabilized diaminobenzidine (DAB) solution, are combined with the tissue section for a time sufficient for color development. Following rinsing, the tissue section is ready for analysis.
摘要翻译： PCT No.PCT / US91 / 01108 Sec。 371日期：1992年8月31日 102（e）日期1992年8月31日PCT 1991年2月27日PCT PCT。出版物WO91 / 13336 1991年9月5日。本发明提供了一种使用免疫化学试剂染色载玻片的改进方法。该方法包括以下步骤。载玻片的测定区域（含有组织切片的区域）用包含水和洗涤剂的改进的冲洗溶液洗涤。将蒸发抑制剂液体施加到载玻片上以覆盖测定区域。对于需要揭发的抗原，将组织切片与改良的稳定的蛋白水解酶溶液组合。将载玻片漂洗，并将蒸发抑制剂液体重新施加到载玻片上。将来自与第二抗体相同物种的球蛋白的改进的稀释剂中的一抗与组织切片组合足够的时间，足以基本上完全抗体结合。将载玻片漂洗，并重新施加蒸发抑制剂液体。将改进的稀释剂中的标记的第二抗体与组织切片组合足够长的时间以使基本上完全的抗体结合。将载玻片漂洗，并将蒸发抑制剂液体重新施加到载玻片上。在优选的实施方案中，将显色剂（包括稳定的二氨基联苯胺（DAB）溶液）与组织切片组合足够的时间用于显色。冲洗后，组织部分准备好进行分析。

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