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1. 发明申请

US20050287594A1 Proteomic analysis 审中-公开
标题翻译：蛋白质组学分析
公开(公告)号：US20050287594A1
公开(公告)日：2005-12-29
申请号：US09738271
申请日：2000-12-15
申请人： Benjamin Cravatt , Erik Sorensen , Matthew Patricelli , Martha Lovato , Gregory Adam
发明人： Benjamin Cravatt , Erik Sorensen , Matthew Patricelli , Martha Lovato , Gregory Adam
IPC分类号： G01N33/50 , C07C309/66 , C07C309/73 , C07D495/04 , C07K14/00 , C12Q1/25 , C12Q1/26 , C12Q1/32 , C12Q1/34 , C12Q1/37 , C40B20/04 , C40B30/04 , C40B40/02 , C40B40/04 , C40B50/06 , G01N33/15 , G01N33/535 , G01N33/557 , G01N33/68 , G06F19/18 , G01N33/53 , G01N33/543
CPC分类号： C40B30/04 , C12Q1/26 , C12Q1/32 , C12Q1/37 , C40B40/04 , G01N33/535 , G01N33/557 , G01N33/6842 , G01N33/6845 , G01N2500/20 , G16B20/00 , Y10T436/16 , Y10T436/18
摘要： The present invention provides methods for analyzing proteomes, as cells or lysates. The analysis is based on the use of probes that have specificity to the active form of proteins, particularly enzymes and receptors. The probes can be identified in different ways. In accordance with the present invention, a method is provided for generating and screening compound libraries that are used for the identification of lead molecules, and for the parallel identification of their biological targets. By appending specific functionalities and/or groups to one or more binding moieties, the reactive functionalities gain binding affinity and specificity for particular proteins and classes of proteins. Such libraries of candidate compounds, referred to herein as activity-based probes, or ABPs, are used to screen for one or more desired biological activities or target proteins.
摘要翻译：本发明提供了分析蛋白质组，如细胞或裂解物的方法。分析基于对蛋白质，特别是酶和受体的活性形式具有特异性的探针的使用。可以以不同的方式识别探针。根据本发明，提供了用于产生和筛选用于鉴定铅分子的化合物文库以及用于平行鉴定其生物学靶标的方法。通过将特定的官能团和/或基团附加到一个或多个结合部分，反应性官能团对特定的蛋白质和类别的蛋白质获得结合亲和力和特异性。候选化合物的文库（本文称为基于活性的探针）或ABP用于筛选一种或多种所需生物活性或靶蛋白。

2. 发明授权

US07179655B2 Protein profiling platform 有权
标题翻译：蛋白质分析平台
公开(公告)号：US07179655B2
公开(公告)日：2007-02-20
申请号：US10087602
申请日：2002-03-01
申请人： Matthew Patricelli
发明人： Matthew Patricelli
IPC分类号： G01N24/00
CPC分类号： G01N33/6848 , G01N30/7233 , G01N30/74 , G01N30/82 , G01N30/8675 , G01N33/6818 , G01N33/6842 , G01N33/6851 , Y10T436/24 , Y10T436/25125 , G01N21/64
摘要： Methods and compositions are described for analyzing complex protein mixtures, such as proteomes, using activity-based probes. In particular, probes that specifically react with and bind to the active form of one or more target proteins are employed. Labeled peptides obtained from the labeled active target proteins can be used in screening and identification procedures, and can be related to the identity, presence, amount, or activity of active members of the desired target protein class. The methods and compositions described herein can be used, for example, to provide diagnostic information concerning pathogenic states, in identifying proteins that may act as therapeutic targets, and in drug discovery.
摘要翻译：描述了使用基于活性的探针分析复合蛋白质混合物（如蛋白质组）的方法和组合物。特别地，使用与一种或多种靶蛋白的活性形式特异性反应并结合的探针。从标记的活性靶蛋白获得的标记肽可以用于筛选和鉴定程序，并且可以与期望的靶蛋白质类的活性成员的身份，存在，量或活性相关。本文描述的方法和组合物可用于例如提供关于致病状态的诊断信息，鉴定可用作治疗靶标的蛋白质，以及在药物发现中。

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