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1. 发明授权

US4722889A Immunoassays using multiple monoclonal antibodies and scavenger antibodies 失效
标题翻译：使用多种单克隆抗体和清道夫抗体进行免疫测定
公开(公告)号：US4722889A
公开(公告)日：1988-02-02
申请号：US718921
申请日：1985-04-02
申请人： Jin P. Lee , F. Brad Salcedo , Martin F. Robins
发明人： Jin P. Lee , F. Brad Salcedo , Martin F. Robins
IPC分类号： G01N33/543 , G01N33/577 , G01N33/76
CPC分类号： G01N33/577 , G01N33/54393 , G01N33/76 , Y10S435/81 , Y10S435/962 , Y10S435/971 , Y10S435/975
摘要： A method and reagent kit means are provided for assay of a selected antigen such as hCG or CEA in an aliquot of body fluid. The method comprises the steps of constituting the aliquot in a mixture comprising tracer (which may be an enzyme tracer or a radioactive tracer) conjugated with monoclonal antibody, and separate immobilized monoclonal antibody, incubating the mixture to enable separation of a solid phase antigen antibody conjugate in sandwich relation, and measuring the tracer content and corresponding antigen content of the aqueous phase or the solid phase. The antibody (conjugated and/or immobilized) comprises multiple monoclonal antibodies from different cell lines so that the specificity of the assay is enhanced, and the possibility of unrecognized antigen fragments is reduced. Also, as a preferred option, the incubation may be carried out with a scavenger monoclonal antibody so that, as an example, in the context of hCG assay, the scavenger chosen for beta subunit selectivity but low hCG affinity is present in the reaction to prevent any possible cross reactivity from analogs of homologous reactivity.
摘要翻译：提供了一种方法和试剂盒装置，用于在体液等分试样中测定所选抗原如hCG或CEA。该方法包括以下步骤：在与单克隆抗体缀合的示踪剂（其可以是酶示踪剂或放射性示踪剂）和分离的固定化单克隆抗体的混合物中构成等分试样，将混合物孵育以使得能够分离固相抗原结合物夹心关系，并测量水相或固相的示踪剂含量和相应的抗原含量。抗体（缀合和/或固定化）包含来自不同细胞系的多种单克隆抗体，从而增强了测定的特异性，降低了未识别的抗原片段的可能性。此外，作为优选的选择，可以用清道夫单克隆抗体进行培养，使得作为实例，在hCG测定的背景下，选择用于β亚基选择性但低hCG亲和力的清除剂存在于反应中以防止与同源反应性类似物的任何可能的交叉反应性。

2. 发明授权

US5026653A Scavenger antibody mixture and its use for immunometric assay 失效
标题翻译：清道夫抗体混合物及其用于免疫测定
公开(公告)号：US5026653A
公开(公告)日：1991-06-25
申请号：US103067
申请日：1987-09-30
申请人： Jin P. Lee , F. Brad Salcedo , Martin F. Robins
发明人： Jin P. Lee , F. Brad Salcedo , Martin F. Robins
IPC分类号： G01N33/543 , G01N33/577 , G01N33/76
CPC分类号： G01N33/577 , G01N33/54393 , G01N33/76 , Y10S435/962 , Y10S436/807 , Y10S436/814 , Y10S436/818
摘要： Provided is a test kit for conducting a membrane sandwich immunoassay of a multivalent antigen, optionally including reagents, the test kit includes a hollow container for reception of a fluid specimen, containing in series fluid absorbent body, a fluid pervious layer, and a fluid pervious solid membrane layer on which antibody is immobilized such that fluid added (e.g., labeled antibody and fluid specimen) to the container migrates through the membrane and in the presence of unlabeled scavenger antibody on the reactive surface of the membrane forms a multiple-site immunospecific antibody-antigen-antibody sandwich immobilized and labeled thereon, preferably in the form of a visible positive or negative indicator of the presence and/or amount of the analyte antigen in the specimen sample. The scavenger antibody serves to prevent false positive or other spurious reactions due to antigen analogs, antigen interference substances, or circulating human immnoglobulins.
摘要翻译：提供了用于进行多价抗原的膜夹心免疫测定的测试试剂盒，任选地包括试剂，测试试剂盒包括用于接收流体试样的中空容器，其包含串联流体吸收体，流体可渗透层和流体渗透层固定有抗体的固体膜层，使得向容器加入的流体（例如，标记的抗体和流体样品）在膜上迁移并且在膜的反应性表面上存在未标记的清除剂抗体形成多位点免疫特异性抗体固定化并在其上标记的抗原 - 抗体夹心剂，优选以可见的阳性或阴性指示剂的形式存在和/或样本样品中分析物抗原的量。清道夫抗体用于防止由于抗原类似物，抗原干扰物质或循环的人类非球蛋白引起的假阳性或其他假阳性反应。

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