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    • 6. 发明申请
    • Differential PCR-RFLP assay for detecting and distinguishing between nonpathogenic PCV-1 and pathogenic PCV-2
    • 差异PCR-RFLP检测和鉴别非致病性PCV-1与致病性PCV-2
    • US20050147966A1
    • 2005-07-07
    • US11040447
    • 2005-01-21
    • Xiang-Jin MengMartijn Fenaux
    • Xiang-Jin MengMartijn Fenaux
    • C12Q1/68C12Q1/70C07H21/04C12P19/34
    • C12Q1/701C12Q1/683
    • The present invention relates to a method for detecting and differentiating PCV infections in a biological sample taken from a pig which involves amplifying a fragment from an extracted nucleic acid; digesting the fragment with a suitable restriction enzyme such as the unique NcoI restriction enzyme; forming a restriction fragment length polymorphism pattern; and then detecting the presence or absence of a PCV isolate. The invention further concerns the new oligonucleotide primers for differentiating PCV infections comprising a nucleotide sequence selected from the group consisting of MCV1 having a nucleotide sequence set forth in SEQ ID NO:1 and MCV2 having a nucleotide sequence set forth in SEQ ID NO:2. Moreover, this invention provides a novel kit for detecting and distinguishing PCV infections that includes the new oligonucleotide primers and the suitable restriction enzyme.
    • 本发明涉及一种用于检测和分化取自猪的生物样品中的PCV感染的方法,其涉及从提取的核酸扩增片段; 用合适的限制酶如独特的NcoI限制酶消化片段; 形成限制性片段长度多态性模式; 然后检测PCV分离物的存在或不存在。 本发明还涉及用于分化PCV感染的新的寡核苷酸引物,其包含选自具有SEQ ID NO:1所示核苷酸序列的MCV1和具有SEQ ID NO:2所示核苷酸序列的MCV2的核苷酸序列。 此外,本发明提供了用于检测和鉴别PCV感染的新型试剂盒,其包括新的寡核苷酸引物和合适的限制酶。