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1. 发明申请

US20070105162A1 Monovalent avidin and streptavidin compositions 审中-公开
标题翻译：单价抗生物素蛋白和链霉亲和素组合物
公开(公告)号：US20070105162A1
公开(公告)日：2007-05-10
申请号：US11588879
申请日：2006-10-27
申请人： Alice Ting , Mark Howarth
发明人： Alice Ting , Mark Howarth
IPC分类号： C07K14/705 , G01N33/53 , C07H21/04 , C12P21/06
CPC分类号： C07K14/465 , C07K14/36
摘要： The invention relates, in part, to monovalent avidin and streptavidin compositions. The invention also relates to methods of preparing and using monovalent avidin and streptavidin compositions. In some aspects of the invention, the compositions are monovalent avidin or monovalent streptavidin with a single femtomolar biotin-binding site.
摘要翻译：本发明部分涉及单价抗生物素蛋白和链霉亲和素组合物。本发明还涉及制备和使用一价抗生物素蛋白和链霉亲和素组合物的方法。在本发明的某些方面，组合物是具有单个生物素结合位点的单价抗生物素蛋白或单链链霉抗生物素蛋白。

2. 发明授权

US1390132A Continuous wire-drawing machinery 失效
标题翻译：连续拉丝机械
公开(公告)号：US1390132A
公开(公告)日：1921-09-06
申请号：US39605320
申请日：1920-07-14
申请人： MARK HOWARTH
发明人： MARK HOWARTH
IPC分类号： B21C1/10
CPC分类号： B21C1/10

3. 发明授权

US1355745A Means for drawing wire and rod 失效
标题翻译：用于拉丝和拉杆的方法
公开(公告)号：US1355745A
公开(公告)日：1920-10-12
申请号：US33277719
申请日：1919-10-23
申请人： MARK HOWARTH
发明人： MARK HOWARTH
IPC分类号： B21C3/04
CPC分类号： B21C3/04

4. 发明授权

US09547003B2 Peptide tag systems that spontaneously form an irreversible link to protein partners via isopeptide bonds 有权
标题翻译：通过多肽键自发形成与蛋白质伴侣不可逆链接的肽标签系统
公开(公告)号：US09547003B2
公开(公告)日：2017-01-17
申请号：US13578070
申请日：2011-02-11
申请人： Mark Howarth
发明人： Mark Howarth
IPC分类号： C07K14/315 , G01N33/531
CPC分类号： G01N33/531 , C07K7/08 , C07K14/315 , C07K2319/21 , C07K2319/70 , G01N33/5306
摘要： Described herein is a protein capable of spontaneously forming an isopeptide bond for the development of a peptide tag and binding partner pair wherein the peptide tag and binding partner are capable of covalently binding to each other via an isopeptide bond. Also provided is a method for developing a peptide tag and binding partner pair which are capable of covalently binding to each other based on a protein which is capable of spontaneously forming an isopeptide bond. Additionally provided are peptide tag and binding partner pairs which are obtainable from isopeptide proteins. Further, specifically developed peptide tags and binding partners are described, together with nucleic acid molecules and vectors which encode those peptides or proteins.
摘要翻译：本文描述了能够自发形成用于开发肽标签和结合配偶体对的肽链的蛋白质，其中肽标签和结合配偶体能够经由异肽键彼此共价结合。还提供了一种用于开发能够基于能够自发形成异肽键的蛋白质彼此共价结合的肽标签和结合配偶体对的方法。另外提供可从异肽蛋白获得的肽标签和结合配对对。此外，与编码那些肽或蛋白质的核酸分子和载体一起描述了专门开发的肽标签和结合配偶体。

5. 发明授权

US1406867A Means for drawing wire and rod 失效
标题翻译：用于拉丝和拉杆的方法
公开(公告)号：US1406867A
公开(公告)日：1922-02-14
申请号：US32752419
申请日：1919-09-30
申请人： MARK HOWARTH
发明人： MARK HOWARTH
IPC分类号： B21C3/12
CPC分类号： B21C3/12

6. 发明授权

US08759488B2 High stability streptavidin mutant proteins 有权
标题翻译：高稳定性链霉抗生物素蛋白突变蛋白
公开(公告)号：US08759488B2
公开(公告)日：2014-06-24
申请号：US13504540
申请日：2010-10-28
申请人： Mark Howarth
发明人： Mark Howarth
IPC分类号： C07K17/00
CPC分类号： C07K14/36
摘要： The present invention provides a mutant streptavidin subunit which comprises one or more amino acid substitutions compared to a wildtype streptavidin subunit at any one of residue positions equivalent to positions (50, 51, 52, 53) and (54) of SEQ ID NO. 2 and wherein amino acid residues at positions equivalent to positions (23, 27, 43, 45, 49, 79, 88, 90, 92, 108, 110) and (128) of SEQ ID NO. 2, in said mutant streptavidin subunit, are wildtype, wherein (i) when said mutant streptavidin subunit unit is comprised in streptavidin, said streptavidin has a lower off rate for biotin or for a biotin conjugate than wildtype streptavidin or (ii) when said mutant streptavidin subunit is in monomelic form said mutant monomelic streptavidin has a lower off rate for biotin or a biotin conjugate than monomelic streptavidin. The invention also encompasses nucleic acid molecules comprising a nucleotide sequence encoding the mutant streptavidin subunit and vectors and cells comprising the nucleic acid. Further, methods of capturing biotinylated molecules and cells and therapeutic methods are encompassed.
摘要翻译：本发明提供了与野生型链霉抗生物素蛋白亚基相当的任何一个等位于SEQ ID NO的位置（50,51,52,53）和（54）的任何一个位置上的一个或多个氨基酸取代的突变链霉亲和素亚基。 2，并且其中相当于SEQ ID NO.1的位置（23,27,43,45,49,79,88,90,92,108,110）和（128）的位置处的氨基酸残基。 2，在所述突变链霉亲和素亚基中，是野生型，其中（i）当所述突变链霉亲和素亚单位包含在链霉亲和素中时，所述链霉抗生物素蛋白与野生型链霉抗生物素蛋白相比，生物素或生物素缀合物的脱除率较低，或（ii）链霉抗生物素蛋白亚基是单体形式，所述突变单体链霉抗生物素蛋白与生物素或生物素缀合物比单体链霉亲和素具有较低的脱除率。本发明还包括包含编码突变链亲和素亚基的核苷酸序列和包含该核酸的载体和细胞的核酸分子。此外，包括捕获生物素化分子和细胞的方法以及治疗方法。

7. 发明授权

US1418186A Manufacture of nails and the like 失效
标题翻译：指甲等的制造
公开(公告)号：US1418186A
公开(公告)日：1922-05-30
申请号：US32752519
申请日：1919-09-30
申请人： MARK HOWARTH
发明人： MARK HOWARTH
IPC分类号： B21G3/00 , B21G3/18
CPC分类号： B21G3/005 , B21G3/18

8. 发明申请

US20130345951A1 ENGINE PERFORMANCE MODIFICATION OR TUNING KIT 审中-公开
标题翻译：发动机性能修改或调试工具包
公开(公告)号：US20130345951A1
公开(公告)日：2013-12-26
申请号：US14003987
申请日：2012-03-09
申请人： Edward Zyla , Mark Howarth
发明人： Edward Zyla , Mark Howarth
IPC分类号： F02D41/26
CPC分类号： F02D41/26 , F02D19/027 , F02D19/061 , F02D19/0647 , F02D19/066 , F02D41/0027 , F02D41/3836 , F02D2041/281 , F02D2200/0602 , F02D2200/101 , F02D2400/11 , Y02T10/32 , Y02T10/36
摘要： A performance modification/tuning kit for an internal combustion engine of the type comprises a kit control unit. An electrical sensor is adapted to detect the opening and closing signals of a control line to one of the injectors of the engine and connected through a sensor line to the control unit. An input receives the signal from a pressure sensor of the common fuel rail of the engine and an output report a generated pressure signal to the engines ECU. A selectively changeable modification map, comprising modification values for a range of different engine speeds and fuel loads demanded by the ECU is used by the kit control unit to generate the generated pressure signal. That is generated in dependence upon the value in the modification map for the engine fuel load Q currently demanded by the ECU and the engine speed currently pertaining from time to time.
摘要翻译：用于这种类型的内燃机的性能改进/调整套件包括套件控制单元。电传感器适用于检测到发动机的喷油器之一的控制线的打开和关闭信号，并通过传感器线连接到控制单元。输入接收来自发动机的公共燃料轨的压力传感器的信号，并且向发动机ECU输出产生的压力信号。由套件控制单元使用包括由ECU要求的不同发动机速度和燃料负荷的范围的修改值来生成所产生的压力信号的可选择地变化的修改图。这是根据ECU当前要求的发动机燃料负荷Q和当前与时间有关的发动机转速的变化图中的值产生的。

9. 发明申请

US20130053544A1 PEPTIDE TAG SYSTEMS THAT SPONTANEOUSLY FORM AN IRREVERSIBLE LINK TO PROTEIN PARTNERS VIA ISOPEPTIDE BONDS 有权
标题翻译：肽类标签系统通过异构体骨架自发形成与蛋白质合作伙伴不可逆转的链接
公开(公告)号：US20130053544A1
公开(公告)日：2013-02-28
申请号：US13578070
申请日：2011-02-11
申请人： Mark Howarth
发明人： Mark Howarth
IPC分类号： C07K19/00 , C12N1/21 , C12N15/11 , C12N15/81 , C12N15/70 , C12P21/02 , C12N1/19
CPC分类号： G01N33/531 , C07K7/08 , C07K14/315 , C07K2319/21 , C07K2319/70 , G01N33/5306
摘要： The present invention concerns the use of a protein capable of spontaneously forming an isopeptide bond for the development of a peptide tag and binding partner pair wherein said peptide tag and binding partner are capable of covalently binding to each other via an isopeptide bond. The invention also provides a method for developing a peptide tag and binding partner pair which are capable of covalently binding to each other based on a protein which is capable of spontaneously forming an isopeptide bond. Additionally provided are peptide tag and binding partner pairs which are obtainable from isopeptide proteins. Further, specifically developed peptide tags and binding partners are encompassed, together with nucleic acid molecules and vectors which encode those peptides or proteins.
摘要翻译：本发明涉及能够自发形成异肽键的蛋白质用于开发肽标签和结合配偶体对的用途，其中所述肽标签和结合配偶体能够经由异肽键彼此共价结合。本发明还提供了能够基于能够自发形成异肽键的蛋白质彼此共价结合的肽标签和结合配偶体对的开发方法。另外提供可从异肽蛋白获得的肽标签和结合配对对。此外，包括编码那些肽或蛋白质的核酸分子和载体的特异性开发的肽标签和结合配偶体。

10. 发明申请

US20120214970A1 High Stability Streptavidin Mutant Proteins 有权
标题翻译：高稳定性链霉亲和素突变蛋白
公开(公告)号：US20120214970A1
公开(公告)日：2012-08-23
申请号：US13504540
申请日：2010-10-28
申请人： Mark Howarth
发明人： Mark Howarth
IPC分类号： C07K14/77 , C12N1/19 , C12N1/21 , C12N15/63 , C12P21/02
CPC分类号： C07K14/36
摘要： The present invention provides a mutant streptavidin subunit which comprises one or more amino acid substitutions compared to a wildtype streptavidin subunit at any one of residue positions equivalent to positions (50, 51, 52, 53) and (54) of SEQ ID NO. 2 and wherein amino acid residues at positions equivalent to positions (23, 27, 43, 45, 49, 79, 88, 90, 92, 108, 110) and (128) of SEQ ID NO. 2, in said mutant streptavidin subunit, are wildtype, wherein (i) when said mutant streptavidin subunit unit is comprised in streptavidin, said streptavidin has a lower off rate for biotin or for a biotin conjugate than wildtype streptavidin or (ii) when said mutant streptavidin subunit is in monomelic form said mutant monomelic streptavidin has a lower off rate for biotin or a biotin conjugate than monomelic streptavidin. The invention also encompasses nucleic acid molecules comprising a nucleotide sequence encoding the mutant streptavidin subunit and vectors and cells comprising the nucleic acid. Further, methods of capturing biotinylated molecules and cells and therapeutic methods are encompassed.
摘要翻译：本发明提供了与野生型链霉抗生物素蛋白亚基相当的任何一个等位于SEQ ID NO的位置（50,51,52,53）和（54）的任何一个位置上的一个或多个氨基酸取代的突变链霉亲和素亚基。 2，并且其中相当于SEQ ID NO.1的位置（23,27,43,45,49,79,88,90,92,108,110）和（128）的位置处的氨基酸残基。 2，在所述突变链霉亲和素亚基中，是野生型，其中（i）当所述突变链霉亲和素亚单位包含在链霉亲和素中时，所述链霉抗生物素蛋白与野生型链霉抗生物素蛋白相比，生物素或生物素缀合物的脱除率较低，或（ii）链霉抗生物素蛋白亚基是单体形式，所述突变单体链霉抗生物素蛋白与生物素或生物素缀合物比单体链霉亲和素具有较低的脱除率。本发明还包括包含编码突变链亲和素亚基的核苷酸序列和包含该核酸的载体和细胞的核酸分子。此外，包括捕获生物素化分子和细胞的方法以及治疗方法。

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