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1. 发明授权

US5858648A Assays using reference microparticles 失效
标题翻译：使用参考微粒的测定
公开(公告)号：US5858648A
公开(公告)日：1999-01-12
申请号：US743164
申请日：1996-11-04
申请人： James S. Steel , Anne J. Bodner , Manish S. Kochar , Glen M. Ford , John P. Huff
发明人： James S. Steel , Anne J. Bodner , Manish S. Kochar , Glen M. Ford , John P. Huff
IPC分类号： G01N33/543 , C12Q1/70 , G01N33/545 , G01N33/546 , G01N33/554
CPC分类号： G01N33/54313 , Y10S436/805 , Y10S436/806
摘要： Disclosed is an assay for determining the presence of at least one analyte in a sample, which involves the steps of: (a) mixing the sample and predetermined amounts of first test microparticles having disposed thereon a binding molecule which binds a first analyte, and inert reference microparticles to form a reaction mixture, and to allow for the first binding molecule to bind the first analyte; b) counting the numbers of the non-reacted first test microparticles and the reference microparticles; and (c) comparing the number of non-reacted first test microparticles to the reference microparticles to thereby establish a first test value so that the presence of the first analyte in the sample may be determined. In a preferred embodiment, the first test value is compared to a control value for a non-reactive sample, and a change value is calculated therefrom. The presence of additional analytes may be determined by adding second test microparticles having disposed thereon a second binding molecule which binds a second analyte, wherein the second test microparticles are resolvable against the first test microparticles and the reference microparticles in the given counting method, and comparing the number of non-reacted second test microparticles to the reference microparticles to establish a second test value. Also disclosed are compositions and kits containing the microparticles.
摘要翻译：公开了一种用于确定样品中至少一种分析物的存在的测定法，其包括以下步骤：（a）将样品和预定量的第一测试微粒混合在其上，其上设置有结合第一分析物的结合分子和惰性的参考微粒以形成反应混合物，并允许第一结合分子结合第一分析物; b）计数未反应的第一测试微粒和参考微粒的数目; 和（c）将未反应的第一测试微粒的数量与参考微粒进行比较，从而建立第一测试值，从而可以确定样品中第一分析物的存在。在优选实施例中，将第一测试值与非反应性样品的对照值进行比较，并从中计算出变化值。附加分析物的存在可以通过加入其上设置有结合第二分析物的第二结合分子的第二测试微粒来确定，其中第二测试微粒可按照给定的计数方法与第一测试微粒和参考微粒分辨，并且比较将未反应的第二测试微粒的数量与参考微粒相比建立第二测试值。还公开了含有微粒的组合物和试剂盒。

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