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    • 3. 发明申请
    • METHOD FOR PREPARING DNA FRAGMENT HAVING STICKY END
    • 用于制备具有末端的DNA片段的方法
    • US20110009607A1
    • 2011-01-13
    • US12921930
    • 2009-03-10
    • Makoto KomiyamaAkinori KuzuyaKeita Tanaka
    • Makoto KomiyamaAkinori KuzuyaKeita Tanaka
    • C12P19/34C07H21/04C07C205/06C07D239/54C07D473/28C07H19/09C07H19/19
    • C12N15/64C12N15/66C12Q1/686C12Q2565/519C12Q2525/117
    • The present invention provides a method for preparing a DNA fragment, in which a desired double-stranded DNA fragment having a sticky end is directly and easily obtained from an amplification product (an amplified fragment) after PCR without a restriction enzyme digestion. The method for preparing a DNA fragment having a sticky end of the present invention comprises: (i) a step of performing a PCR reaction using a template DNA and specific primers to obtain an amplified DNA fragment; and (ii) a step of performing a prescribed treatment on the amplified DNA fragment to dissociate a protecting group from the fragment. Herein, the above-mentioned specific primers are composed of a complementary DNA portion consisting of a nucleotide sequence complementarily binding to an amplification target region in a template DNA and a non-complementary DNA portion consisting of a nucleotide sequence that links to the 5′ end of the complementary DNA portion but does not complementarily bind to the amplification target sequence, and at least a base corresponding to the 3′ end in the nucleotide sequence of the non-complementary DNA portion is modified with a protecting group capable of terminating the progression of DNA replication catalyzed by a DNA polymerase.
    • 本发明提供了一种制备DNA片段的方法,其中在没有限制酶消化的PCR之后,从扩增产物(扩增片段)直接容易地获得具有粘性末端的所需双链DNA片段。 制备本发明粘性末端的DNA片段的方法包括:(i)使用模板DNA和特异性引物进行PCR反应以获得扩增的DNA片段的步骤; 和(ii)对扩增的DNA片段进行规定的处理以从保护基离解的步骤。 本文中,上述特异性引物由与模板DNA中的扩增对象区域互补结合的核苷酸序列和与5'末端连接的核苷酸序列组成的非互补DNA部分构成的互补DNA部分构成 的互补DNA部分,但不与扩增靶序列互补结合,并且至少对应于非互补DNA部分的核苷酸序列中3'端的碱基用能够终止进展的保护基进行修饰 由DNA聚合酶催化的DNA复制。
    • 5. 发明授权
    • Process for separating carbon monoxide from a gas mixture
    • 从气体混合物中分离一氧化碳的方法
    • US4460384A
    • 1984-07-17
    • US373241
    • 1982-04-29
    • Hidefumi HiraiMakoto KomiyamaSusumu Hara
    • Hidefumi HiraiMakoto KomiyamaSusumu Hara
    • B01D53/14C01B32/40B01D19/00
    • C01B31/18B01D53/1493
    • A process for separating carbon monoxide from a gas mixture is presented. In this process carbon monoxide contained in the gas mixture containing carbon monoxide and up to approximately 40,000 ppm of water can be effectively separated by contacting the gas mixture with an absorbing solution containing (a) at least one copper(I) halide, (b) at least one aluminum(III) halide and (c) at least one compound having at least two benzene nuclei in one molecule thereof. The component (c) is selected from the group consisting of (A) the compounds having the general formula (I): ##STR1## wherein R.sub.1 is hydrogen or an alkyl group, R.sub.2 and R.sub.3 are independently hydrogen, an alkyl group, or an aryl group, i is 0, 1, 2, 3, or 4, and m and n are independently 0, 1, 2, or 3, (B) the oligomers styrene or the derivatives thereof, (C) the polymers of styrene or the derivatives thereof and (D) copolymers of (i) styrene or the derivatives thereof and (ii) ethylene or the derivatives thereof and, optionally, an aromatic solvent, without causing the decrease in the carbon monoxide absorbing and releasing capacities of the absorbing solution.
    • 提出了从气体混合物中分离一氧化碳的方法。 在该方法中,通过使气体混合物与含有(a)至少一种卤化铜(I)的吸收溶液接触,(b)将含有一氧化碳和高达约40,000ppm水的气体混合物中所含的一氧化碳, 至少一种卤化铝(III)和(c)至少一种在其一个分子中具有至少两个苯核的化合物。 组分(c)选自(A)具有通式(I)的化合物:其中R 1是氢或烷基,R 2和R 3独立地是氢,烷基 ,或芳基,i为0,1,2,3或4,m和n分别为0,1,2或3,(B)低聚物苯乙烯或其衍生物,(C)聚合物 的苯乙烯或其衍生物,和(D)(i)苯乙烯或其衍生物和(ii)乙烯或其衍生物和任选的芳族溶剂的共聚物,而不会导致一氧化碳吸收和释放能力的降低 吸收溶液。
    • 7. 发明授权
    • Optical-fiber inspection device
    • 光纤检测装置
    • US6008487A
    • 1999-12-28
    • US39944
    • 1998-03-16
    • Yoshihiko TachikawaYoshihiro SampeiTakaaki HirataMakoto KomiyamaYasuyuki SuzukiMamoru Arihara
    • Yoshihiko TachikawaYoshihiro SampeiTakaaki HirataMakoto KomiyamaYasuyuki SuzukiMamoru Arihara
    • G01M11/00H01J5/16
    • G01M11/3172
    • In the primary invention of this application, an optical-fiber inspection device which detects the distance to a reflection point within the DUT and the amount of reflected light by dividing into two a laser beam which is possible to be frequency-swept, making one of the divided light beams incident to an optical detector via the reference light path, while making the other of the divided light beams incident to the DUT and making the light reflected within the DUT incident to the said optical detector, and analyzing the frequency of the interference signal for the two beams obtained by the optical detector, is configured so that the final reference light is obtained by providing an optical coupler in the reference light path and, after taking out a part of the reference light and making it pass through an optical frequency shifter, combining this again with the original reference light with the said optical coupler.
    • 在本申请的第一个发明中,一种光纤检查装置,其检测到DUT内的反射点的距离和通过分成两个可能被频率扫描的激光束的反射光的量,使得 通过参考光路入射到光学检测器的分束光束,同时使分离的光束中的另一个入射到DUT并使在DUT中反射的光入射到所述光学检测器,并且分析干扰的频率 由光检测器获得的两束光信号被配置为使得最终参考光通过在参考光路中提供光耦​​合器而获得,并且在取出一部分参考光并使其通过光频率 移位器,再次与原来的参考光与所述光耦合器组合。
    • 8. 发明授权
    • Process for producing a para-substituted phenol derivative
    • 对位取代苯酚衍生物的制备方法
    • US4663478A
    • 1987-05-05
    • US725360
    • 1985-04-18
    • Hidefuni HiraiMakoto KomiyamaIzuru Sugiura
    • Hidefuni HiraiMakoto KomiyamaIzuru Sugiura
    • C07C37/11C07C37/18C07C45/00C07C45/51C07C45/74C07C51/00C07C51/093C07C65/04
    • C07C51/00C07C45/00C07C45/008C07C45/512C07C45/74C07C51/093
    • By reacting a phenol compound with an organic halide selected from the group consisting of a haloform, a carbon tetrahalide and a substituted or unsubstituted allyl halide in the presence of an alkali metal hydroxide, using as a catalyst a fixed cyclodextrin having hydroxyl groups crosslinked with a bivalent hydrocarbon group having free valences at its both ends, said hydrocarbon group having at least one hydrogen atom substituted or unsubstituted with a member selected from the group consisting of an alkyl group, a halogen atom and a hydroxyl group and containing or not containing at least one combination of two neighboring carbon atoms having therebetween at least one member selected from the group consisting of an oxygen atom, a sulfur atom and a phenylene, various useful para-substituted phenol derivatives can be advantageously obtained. The fixed cyclodextrin which has been used as the catalyst can be extremely easily separated from the reaction system without any loss of the fixed cyclodextrin, and the recovered fixed cyclodextrin can be used repeatedly as a catalyst in the process of the present invention without lowering in yield of and selectivity for para-substituted phenol derivatives.
    • PCT No.PCT / JP85 / 00057 Sec。 371日期:1985年4月18日 102(e)日期1985年4月18日PCT提交1985年2月13日PCT公布。 公开号WO85 / 03701 1985年8月29日。在碱金属氢氧化物的存在下,使苯酚化合物与选自卤代甲酰基,四卤化碳和取代或未取代的烯丙基卤的有机卤化物反应,使用固定 具有在其两端与具有游离价的二价烃基交联的羟基的环糊精,所述烃基具有至少一个被选自烷基,卤素原子和羟基的成员所取代或未取代的氢原子 并且含有或不包含两个相邻碳原子的至少一个组合,其间具有选自氧原子,硫原子和亚苯基中的至少一个的成员,可以有利地获得各种有用的对位取代的酚衍生物。 已经用作催化剂的固定的环糊精可以非常容易地从反应体系中分离出来,而没有固定的环糊精的任何损失,并且回收的固定的环糊精可以在本发明的方法中作为催化剂反复使用,而不降低产率 的对位取代的苯酚衍生物的选择性。
    • 9. 发明授权
    • Method for separating carbon dioxide from mixed gas
    • 从混合气体中分离二氧化碳的方法
    • US4587114A
    • 1986-05-06
    • US475258
    • 1983-03-14
    • Hidefumi HiraiMakoto KomiyamaKeiichiro Wada
    • Hidefumi HiraiMakoto KomiyamaKeiichiro Wada
    • B01J20/20C01B32/40B01J21/18
    • C01B31/18B01J20/20
    • A solid form adsorbent of carbon monoxide, that is obtained by the process including the following steps. First, copper salts or copper oxide is stirred in a solvent. Then, into the solution or suspension thus obtained, active carbon is added. Finally, the solvent is removed by means of pressure reduction, distillation, etc. The carbon monoxide adsorbent produced according to this invention adsorbs carbon monoxide rapidly when it is brought into contact with a mixed gas. The adsorbed carbon monoxide can be readily separated and discharged through either heating the carbon monoxide adsorbent to above 60.degree. C. or reducing the partial pressure of carbon monoxide. The carbon monoxide obtained in accordance with the present invention is stable relative to the water content in the mixed gas, and it is capable of separating carbon monoxide directly from the mixed gas containing the water. Also, the separated carbon monoxide does not contain the vapor of the solvent from the carbon monoxide adsorbent. Therefore, it is not necessary to provide equipment to collect the solvent vapor. Furthermore, this carbon monoxide adsorbent does not produce corrosive gases, etc. The carbon monoxide adsorbent obtained by using copper (II) salts or copper (II) oxide is also stable relative to sulfur content in the mixed gas.
    • 一氧化碳的固体形式吸附剂,其通过包括以下步骤的方法获得。 首先,将铜盐或氧化铜在溶剂中搅拌。 然后,加入如此获得的溶液或悬浮液中,加入活性炭。 最后,通过减压,蒸馏等除去溶剂。根据本发明生产的一氧化碳吸附剂在与混合气体接触时迅速吸附一氧化碳。 吸附的一氧化碳可以通过将一氧化碳吸附剂加热至60℃以上或者降低一氧化碳的分压来容易地分离和排出。 根据本发明获得的一氧化碳相对于混合气体中的含水量是稳定的,并且能够直接从含有水的混合气体中分离一氧化碳。 此外,分离的一氧化碳不含来自一氧化碳吸附剂的溶剂的蒸气。 因此,不需要提供收集溶剂蒸汽的设备。 此外,该一氧化碳吸附剂不产生腐蚀性气体等。使用铜(II)盐或氧化铜(II))得到的一氧化碳吸附剂相对于混合气体中的硫含量也是稳定的。