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    • 3. 发明授权
    • Brassica or helianthus plants having mutant delta-12 or delta-15 sequences
    • 芸苔属或具有突变体δ-12或δ-15序列的向日葵植物
    • US07135614B1
    • 2006-11-14
    • US08572027
    • 1995-12-14
    • Lorin R. DeBonteZhegong FanGuo-Hua Miao
    • Lorin R. DeBonteZhegong FanGuo-Hua Miao
    • A01H5/00A01H1/06C07H21/02
    • C12N9/0083C12N15/8247
    • Plants are disclosed that contain a mutation in a delta-12 or delta-15 fatty acid desaturase gene. Preferred plants are rapeseed and sunflower plants. Plants carrying such mutant genes have altered fatty acid composition in seeds. In one embodiment, a plant contains a mutation in a region having the conserved motif His-Xaa-Xaa-His, found in delta-12 and delta-15 fatty acid desaturases. A preferred motif has the sequence His-Glu-Cys-Gly-His. A preferred mutation in this motif has the amino acid sequence His-Lys-Cys-Gly-His. Nucleic acid fragments are disclosed that comprise a sequence of at least 20 nucleotides from a mutant delta-12 or delta-15 fatty acid desaturase gene.
    • 公开了在δ-12或δ-15脂肪酸去饱和酶基因中含有突变的植物。 优选的植物是油菜籽和向日葵植物。 携带这种突变基因的植物已经改变了种子中的脂肪酸组成。 在一个实施方案中,植物在具有保守基序His-Xaa-Xaa-His,在δ-12和δ-15脂肪酸去饱和酶中发现的区域中含有突变。 优选的基序具有序列His-Glu-Cys-Gly-His。 该基序中优选的突变具有氨基酸序列His-Lys-Cys-Gly-His。 公开了包含来自突变体Δ-12或δ-15脂肪酸去饱和酶基因的至少20个核苷酸的序列的核酸片段。
    • 9. 发明授权
    • Fatty acid desaturases and mutant sequences thereof
    • 脂肪酸去饱和酶及其突变体序列
    • US06342658B1
    • 2002-01-29
    • US09354231
    • 1999-07-16
    • Lorin R. DeBonteBasil S. Shorrosh
    • Lorin R. DeBonteBasil S. Shorrosh
    • A01H400
    • C12N9/0083C07C53/126C07C57/02C07C57/03C12N15/8247
    • Seeds, plants and oils are provided having high oleic acid; low linoleic acid; and low linoleic acid plus linolenic acid; and advantageous functional or nutritional properties. Plants are disclosed that contain a mutation in a delta-12 or delta-15 fatty acid desaturase gene. Preferred plants are rapeseed and sunflower plants. Plants carrying such mutant genes have altered fatty acid composition in seeds. In one embodiment, a plant contains a mutation in a region having the conserved motif His-Xaa-Xaa-Xaa-His, found in delta-12 and delta-15 fatty acid desaturases. A preferred motif has the sequence His-Glu-Cys-Gly-His. A preferred mutation in this motif has the amino acid sequence His-Lys-Cys-Gly-His. Nucleic acid fragments are disclosed that comprise a mutant delta-12 or delta-15 fatty acid desaturase gene sequence.
    • 提供具有高油酸的种子,植物和油; 低亚油酸; 低亚油酸加亚麻酸; 和有利的功能或营养特性。 公开了在δ-12或δ-15脂肪酸去饱和酶基因中含有突变的植物。 优选的植物是油菜籽和向日葵植物。 携带这种突变基因的植物已经改变了种子中的脂肪酸组成。 在一个实施方案中,植物在具有保守基序His-Xaa-Xaa-Xaa-His的区域中含有突变,其在Δ-12和δ-15脂肪酸去饱和酶中发现。 优选的基序具有序列His-Glu-Cys-Gly-His。 该基序中优选的突变具有氨基酸序列His-Lys-Cys-Gly-His。 公开了包含突变体Δ-12或δ-15脂肪酸去饱和酶基因序列的核酸片段。