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1. 发明授权

US5695970A Glucan lyase producing 1,5-anhydrofructose 失效
标题翻译：葡聚糖裂解酶产生1,5-脱水果糖
公开(公告)号：US5695970A
公开(公告)日：1997-12-09
申请号：US416709
申请日：1995-04-18
申请人： Shukun Yu , Marianne Pedersen , Lennart Kenne
发明人： Shukun Yu , Marianne Pedersen , Lennart Kenne
IPC分类号： C07H3/10 , C12N9/88 , C12P19/02 , C12N1/20
CPC分类号： C12P19/02 , C07H3/10 , C12N9/88
摘要： The invention relates to an enzyme, exo-.alpha.-1,4-glucan lyase, capable of successively cleaving the terminal .alpha.-1,4-D-glucosidic bonds from the non-reducing ends of an .alpha.-1,4-glucan. The enzyme is selected from the group consisting of .alpha.-1,4-glucan lyase isolated from an alga and functional derivatives and analogs derived from a nucleotide sequence related to an alga .alpha.-1,4-glucan lyase. Further, a method of enzymatically cleaving the terminal .alpha.-1,4-D-glucosidic bonds from the non-reducing ends of an .alpha.-1,4-glucan, and the degradation product 1,5-anhydrofructose for use as an oxygen radical scavenger or anti-oxidant and/or as a sugar substitute, are disclosed. Additionally, a method of producing an enzyme capable of successively cleaving the terminal .alpha.-1,4-glucosidic bonds from the non-reducing ends of an .alpha.-1,4-glucan; an antibody which binds to the amino acid sequence of the enzyme, and a DNA or RNA probe which recognizes a nucleotide sequence coding for the enzyme, are described.
摘要翻译： PCT No.PCT / SE93 / 00854 Sec。 371日期1995年04月18日 102（e）1995年4月18日PCT PCT 1993年10月19日PCT公布。出版物WO94 / 09122 日期1994年04月28日本发明涉及能够从α-1的非还原末端连续切割末端α-1,4-D-糖苷键的酶，外-α-1,4-葡聚糖裂解酶，4-葡聚糖。该酶选自由藻类分离的α-1,4-葡聚糖裂解酶和功能性衍生物以及衍生自与藻类α-1,4-葡聚糖裂解酶相关的核苷酸序列的类似物。此外，从α-1,4-葡聚糖的非还原末端酶切地切割末端α-1,4- D - 糖苷键的方法，以及用作氧自由基的降解产物1,5-脱水果糖清除剂或抗氧化剂和/或作为糖代用品。另外，生产能够从α-1,4-葡聚糖的非还原性末端依次切断末端α-1,4-糖苷键的酶的方法; 描述了结合酶的氨基酸序列的抗体和识别编码该酶的核苷酸序列的DNA或RNA探针。

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