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1. 发明授权

US10066217B2 Thermophilic organisms for conversion of lignocellulosic biomass to ethanol 有权
公开(公告)号：US10066217B2
公开(公告)日：2018-09-04
申请号：US12299070
申请日：2007-05-01
申请人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Kara Podkaminer , John Bardsley , David Anthony Hogsett
发明人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Kara Podkaminer , John Bardsley , David Anthony Hogsett
IPC分类号： C12N9/10 , C12N9/88 , C12N9/12 , C12P7/04 , C12P7/16 , C12N9/04 , C12P7/10 , C12R1/145
摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.

2. 发明申请

US20130302870A1 Flowthrough Pretreatment Of Lignocellulosic Biomass And Selective Separation Of Components Using High-Temperature Nanoporous Membranes 审中-公开
标题翻译：使用高温纳米多孔膜进行木质纤维素生物质的流通预处理和成分选择性分离
公开(公告)号：US20130302870A1
公开(公告)日：2013-11-14
申请号：US13704122
申请日：2011-06-20
申请人： Lee R. Lynd , Chaogong Liu , David A. Hogsett
发明人： Lee R. Lynd , Chaogong Liu , David A. Hogsett
IPC分类号： B01D61/14
CPC分类号： B01D61/14 , C08H8/00 , C10G1/002 , C10G1/02 , C10G1/04 , C10G2300/1011 , C10G2300/1014 , C10G2300/807 , C10L1/023 , C12P7/10 , C12P2201/00 , Y02E50/16 , Y02P30/20
摘要： A new and improved biomass conversion system is disclosed using high-temperature flow-though pretreatment and a nanoporous membrane to provide more digestible biomass for subsequent conversion to biofuels.
摘要翻译：公开了一种新的和改进的生物质转化系统，其使用高温流通预处理和纳米多孔膜以提供更可消化的生物质用于随后转化为生物燃料。

3. 发明申请

US20120077239A9 Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol 审中-公开
标题翻译：用于将木质纤维素生物质转化为乙醇的嗜热生物体
公开(公告)号：US20120077239A9
公开(公告)日：2012-03-29
申请号：US12299070
申请日：2007-05-01
申请人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin , Kara Podkaminer , John Bardsley , David Anthony Hogsett
发明人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin , Kara Podkaminer , John Bardsley , David Anthony Hogsett
IPC分类号： C12P7/06 , C12N15/11 , C12N15/00 , C12N1/21
CPC分类号： C12N9/1029 , C12N9/0006 , C12N9/1217 , C12P7/10 , C12R1/145 , C12Y203/01008 , C12Y207/02001 , Y02E50/16 , Y02E50/17
摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.
摘要翻译：本文公开了消耗多种生物质衍生底物的突变嗜热生物。本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。生物可以用于例如在对于纤维素酶活性最佳以在不表达丙酮酸脱羧酶的情况下产生接近理论乙醇产生的温度下进行的嗜热SSF和SSCF反应。

4. 发明授权

US5837506A Continuous process for making ethanol 失效
标题翻译：连续制造乙醇的方法
公开(公告)号：US5837506A
公开(公告)日：1998-11-17
申请号：US916742
申请日：1997-08-19
申请人： Lee R. Lynd , Colin R. South
发明人： Lee R. Lynd , Colin R. South
IPC分类号： C12P7/10
CPC分类号： C12P7/10 , Y02E50/16
摘要： An improved continuous process for producing ethanol from cellulosic materials employs an intermittently agitated, perpetually fed, solids retaining reactor vessel. Cellulosic substrate, catalysts and fermentation agents are introduced into a reaction vessel to form a slurry. The slurry is agitated for a first selected time interval under conditions sufficient to initiate and maintain a fermentation reaction and then allowed to settle during a second selected time interval. At the end of the second selected time interval an ethanol-containing effluent is removed from the vessel. Thereafter, additional cellulosic material, catalysts and fermentation agents are added to the reactor vessel. This operating cycle is repeated virtually perpetually to effect the ethanol production. It can be implemented using a single bioreactor or a cascade of bioreactors.
摘要翻译：用于从纤维素材料生产乙醇的改进的连续方法采用间歇搅动的，永久进料的固体保留反应器容器。将纤维素底物，催化剂和发酵剂引入反应容器中以形成浆料。在足以启动和维持发酵反应的条件下将浆料搅拌第一选定的时间间隔，然后在第二选定的时间间隔内使其沉降。在第二选择的时间间隔结束时，从容器中除去含乙醇的流出物。此后，将另外的纤维素材料，催化剂和发酵剂加入到反应器容器中。这种操作周期几乎永久地重复，以实现乙醇生产。它可以使用单个生物反应器或级联的生物反应器来实现。

5. 发明授权

US5258293A Continuous process for ethanol production from lignocellulosic materials without mechanical agitation 失效
标题翻译：不经机械搅拌从木质纤维素材料生产乙醇的连续方法
公开(公告)号：US5258293A
公开(公告)日：1993-11-02
申请号：US695516
申请日：1991-05-03
申请人： Lee R. Lynd , David A. Hogsett , Gisbert Spieles
发明人： Lee R. Lynd , David A. Hogsett , Gisbert Spieles
IPC分类号： C12P7/10 , C12P7/14
CPC分类号： C12P7/14 , C12P7/10 , Y02E50/16 , Y10S435/813
摘要： An improved and highly productive method of continuously producing ethanol from lignocellulosic substrates is provided. The method involves providing a suitable microbial system within a reaction vessel and adding fermentable substrate to the reactor to form a reaction mixture. The fermentation reaction is allowed to proceed while a quiescent state is maintained within the reactor. During the fermentation, soluble substrate is differentially retained relative to the feed slurry and reaction biocatalysts are retained and internally recycled within the system. Further, while fermenting substrate is retained within the system, it forms a stratified zone within the reactor such that the concentration of actively fermenting substrate is highest at upper portions of the reaction zone and is lowest, near zero, at a lower portion of the reaction zone. Insoluble, fully reacted substrate may be withdrawn from a region near the bottom of the reactor.
摘要翻译：提供了一种从木质纤维素基质连续生产乙醇的改进和高效的方法。该方法包括在反应容器内提供合适的微生物系统，并向反应器中加入可发酵底物以形成反应混合物。允许发酵反应进行，同时在反应器内保持静止状态。在发酵期间，可溶性底物相对于进料浆液差异保留，反应生物催化剂被保留并在系统内部再循环。此外，当发酵底物保留在体系内时，其在反应器内形成分层区域，使得主动发酵底物的浓度在反应区的上部处最高，并且在反应的较低部分处最低，接近零区。可以从反应器底部附近的区域取出不溶的完全反应的底物。

6. 发明申请

US20100075363A1 Recombinant Yeast Strains Expressing Tethered Cellulase Enzymes 审中-公开
标题翻译：表达干扰纤维素酶的重组酵母菌株
公开(公告)号：US20100075363A1
公开(公告)日：2010-03-25
申请号：US12516175
申请日：2007-11-21
申请人： John E.E. McBride , Kristen M. Delault , Lee R. Lynd , Jack T. Pronk
发明人： John E.E. McBride , Kristen M. Delault , Lee R. Lynd , Jack T. Pronk
IPC分类号： C12Q1/02 , C12N1/19 , C12P7/10 , C07H21/04 , C12N15/74
CPC分类号： C12N9/2437 , C12N9/2445 , C12P7/10 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16
摘要： Recombinant yeast strains that saccharify, ferment and grow on insoluble and crystalline forms of cellulose are disclosed herein. The yeast strains express tethered cellulases including cellobiohydrolase, endoglucanase and β-glucosidase. The recombinant organisms are particularly suited for consolidated bioprocessing.
摘要翻译：本文公开了在不溶性和结晶形式的纤维素上糖化，发酵和生长的重组酵母菌株。酵母菌株表达系链纤维素酶，包括纤维二糖水解酶，内切葡聚糖酶和葡萄糖苷酶。重组生物特别适用于固体生物处理。

7. 发明申请

US20100015678A1 Thermophilic Organisms For Conversion of Lignocellulosic Biomass To Ethanol 审中-公开
标题翻译：用于将木质纤维素生物质转化为乙醇的嗜热生物体
公开(公告)号：US20100015678A1
公开(公告)日：2010-01-21
申请号：US12090745
申请日：2006-10-31
申请人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin
发明人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin
IPC分类号： C12P7/10 , C12N1/21 , C12N15/11 , C12N15/00
CPC分类号： C12N9/1029 , C12N9/0006 , C12N9/1217 , C12P7/10 , C12R1/145 , C12Y203/01008 , C12Y207/02001 , Y02E50/16 , Y02E50/17
摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. Both organisms produce near theoretical ethanol yields without expressing pyruvate decarboxylase
摘要翻译：本文公开了消耗多种生物质衍生底物的突变嗜热生物。本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。两种生物体产生近乎理论乙醇产量而不表达丙酮酸脱羧酶

8. 发明申请

US20090239277A1 Thermophillic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol 审中-公开
标题翻译：用于将木质纤维素生物质转化为乙醇的嗜热菌生物
公开(公告)号：US20090239277A1
公开(公告)日：2009-09-24
申请号：US12398876
申请日：2009-03-05
申请人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin
发明人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin
IPC分类号： C12P7/06 , C12N1/21 , C12N15/11 , C12N15/00
CPC分类号： C12N9/1029 , C12N9/0006 , C12N9/1217 , C12P7/10 , C12R1/145 , C12Y203/01008 , C12Y207/02001 , Y02E50/16 , Y02E50/17
摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. Both organisms produce near theoretical ethanol yields without expressing pyruvate decarboxylase
摘要翻译：本文公开了消耗多种生物质衍生底物的突变嗜热生物。本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。两种生物体产生近乎理论乙醇产量而不表达丙酮酸脱羧酶

9. 发明申请

US20090221049A1 Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol 审中-公开
标题翻译：用于将木质纤维素生物质转化为乙醇的嗜热生物体
公开(公告)号：US20090221049A1
公开(公告)日：2009-09-03
申请号：US12299070
申请日：2007-05-01
申请人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin , Kara Podkaminer , John Bardsley , David Anthony Hogsett
发明人： Arthur Josephus Shaw, IV , Sunil G. Desai , Lee R. Lynd , Mikhail V. Tyurin , Kara Podkaminer , John Bardsley , David Anthony Hogsett
IPC分类号： C12P7/06 , C12N1/21 , C12N15/11 , C12N15/00
CPC分类号： C12N9/1029 , C12N9/0006 , C12N9/1217 , C12P7/10 , C12R1/145 , C12Y203/01008 , C12Y207/02001 , Y02E50/16 , Y02E50/17
摘要： Mutant thermophilic organisms that consume a variety of biomass derived substrates are disclosed herein. Strains of Thermoanaerobacterium saccharolyticum with acetate kinase and phosphotransacetylase expression eliminated are disclosed herein. Further, strain ALK1 has been engineered by site directed homologous recombination to knockout both acetic acid and lactic acid production. Continuous culture involving a substrate concentration challenge lead to evolution of ALK1, and formation of a more robust strain designated ALK2. The organisms may be utilized for example in thermophilic SSF and SSCF reactions performed at temperatures that are optimal for cellulase activity to produce near theoretical ethanol yields without expressing pyruvate decarboxylase.
摘要翻译：本文公开了消耗多种生物质衍生底物的突变嗜热生物。本文公开了消除了乙酸激酶和磷酸转乙酰酶表达的酿酒酵母属杆菌菌株。此外，菌株ALK1已经通过位点定向同源重组进行工程化，以敲除乙酸和乳酸生产。涉及底物浓度挑战的连续培养导致ALK1的进化，并形成称为ALK2的更强壮的菌株。生物可以用于例如在对于纤维素酶活性最佳以在不表达丙酮酸脱羧酶的情况下产生接近理论乙醇产生的温度下进行的嗜热SSF和SSCF反应。

10. 发明申请

US20090221042A1 Process for the Treatment of Lignocellulosic Biomass 有权
标题翻译：木质纤维素生物质处理方法
公开(公告)号：US20090221042A1
公开(公告)日：2009-09-03
申请号：US12226763
申请日：2007-04-30
申请人： Bruce E. Dale , Lee R. Lynd , Mark Laser
发明人： Bruce E. Dale , Lee R. Lynd , Mark Laser
IPC分类号： C12P19/02 , C13K1/02 , C12P19/00 , C12P7/02 , C01C1/00 , B01J19/00
CPC分类号： C12P19/02 , C12P7/10 , C12P2201/00 , C13K1/02 , C13K13/002 , Y02E50/16
摘要： A process for the treatment of biomass to render structural carbohydrates more accessible and/or digestible using concentrated ammonium hydroxide with or without anhydrous ammonia addition, is described. The process preferably uses steam to strip ammonia from the biomass for recycling. The process yields of monosaccharides from the structural carbohydrates are good, particularly as measured by the enzymatic hydrolysis of the structural carbohydrates. The monosaccharides are used as animal feeds and energy sources for ethanol production.
摘要翻译：描述了使用具有或不具有无水氨添加的浓氢氧化铵来处理生物质以使结构碳水化合物更易于和/或消化的方法。该方法优选使用蒸汽从生物质中除去氨以进行再循环。来自结构碳水化合物的单糖的方法产率是好的，特别是通过结构碳水化合物的酶水解测量。单糖用作乙醇生产的动物饲料和能源。

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