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1. 发明申请

US20100278746A1 Method for Measuring Neurotransmitters in Vivo 有权
标题翻译：测量体内神经递质的方法
公开(公告)号：US20100278746A1
公开(公告)日：2010-11-04
申请号：US12676912
申请日：2008-09-04
申请人： Quoc-Thang Nguyen , David Kleinfeld , Lee F. Schroeder
发明人： Quoc-Thang Nguyen , David Kleinfeld , Lee F. Schroeder
IPC分类号： A61K49/00
CPC分类号： G01N33/9406 , A61B5/0084 , A61B5/4064
摘要： A method is provided for in vivo detection of a biochemical substance in an animal by culturing neurofluocytes that stably express a receptor of the biochemical substances by transfecting cells with cDNA of the receptor and a tag that will emit a detectable energy in the presence of the biochemical substance, implanting the neurofluocyte into the animal's brain; and detecting the energy emission of the tag. In a first embodiment, the biochemical substance is a neurotransmitter, the tag is a fluophore, and the step of detecting includes forming an opening in the animal's skull and optically detecting fluorescent emissions using a two-photon laser scanning microscope. Multiple biochemical substances can be simultaneously detected by culturing neurofluocytes that express different receptors and have different fluophor tags that produce fluorescent signals at distinguishable wavelengths.
摘要翻译：提供一种用于体内检测动物体内生物化学物质的方法，该方法是通过培养通过用受体的cDNA转染细胞来稳定表达生物化学物质的受体的神经细胞，以及在生物化学物质存在下将发出可检测的能量的标签物质，将神经细胞植入动物的脑中; 并检测标签的能量发射。在第一实施方案中，生物化学物质是神经递质，标签是荧光团，检测步骤包括在动物颅骨中形成开口，并使用双光子激光扫描显微镜光学检测荧光发射。可以通过培养表达不同受体的神经细胞同时检测多种生物化学物质，并具有不同的荧光标记，其在可区分的波长处产生荧光信号。

2. 发明授权

US08663604B2 Method for measuring neurotransmitters in vivo 有权
标题翻译：体内神经递质测定方法
公开(公告)号：US08663604B2
公开(公告)日：2014-03-04
申请号：US12676912
申请日：2008-09-04
申请人： Quoc-Thang Nguyen , David Kleinfeld , Lee F. Schroeder
发明人： Quoc-Thang Nguyen , David Kleinfeld , Lee F. Schroeder
IPC分类号： A61B5/00 , A61B5/1495
CPC分类号： G01N33/9406 , A61B5/0084 , A61B5/4064
摘要： A method is provided for in vivo detection of a biochemical substance in an animal by culturing neurofluocytes that stably express a receptor of the biochemical substances by transfecting cells with cDNA of the receptor and a tag that will emit a detectable energy in the presence of the biochemical substance, implanting the neurofluocyte into the animal's brain; and detecting the energy emission of the tag. In a first embodiment, the biochemical substance is a neurotransmitter, the tag is a fluophore, and the step of detecting includes forming an opening in the animal's skull and optically detecting fluorescent emissions using a two-photon laser scanning microscope. Multiple biochemical substances can be simultaneously detected by culturing neurofluocytes that express different receptors and have different fluophor tags that produce fluorescent signals at distinguishable wavelengths.
摘要翻译：提供一种用于体内检测动物体内生物化学物质的方法，该方法是通过培养通过用受体的cDNA转染细胞来稳定表达生物化学物质的受体的神经细胞，以及在生物化学物质存在下将发出可检测的能量的标签物质，将神经细胞植入动物的脑中; 并检测标签的能量发射。在第一实施方案中，生物化学物质是神经递质，标签是荧光团，检测步骤包括在动物颅骨中形成开口，并使用双光子激光扫描显微镜光学检测荧光发射。可以通过培养表达不同受体的神经细胞同时检测多种生物化学物质，并具有不同的荧光标记，其在可区分的波长处产生荧光信号。

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