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1. 发明授权

US06375817B1 Apparatus and methods for sample analysis 有权
标题翻译：仪器和样品分析方法
公开(公告)号：US06375817B1
公开(公告)日：2002-04-23
申请号：US09293313
申请日：1999-04-16
申请人： Todd A. Taylor , William W. Carson , Lance Koutny
发明人： Todd A. Taylor , William W. Carson , Lance Koutny
IPC分类号： G01N2226
CPC分类号： G01N27/44743 , B01L3/502784 , B01L2200/0605 , B01L2300/0816 , B01L2300/0861 , B01L2400/0415 , B01L2400/0487 , G01N27/44791 , Y10T436/2575
摘要： The present invention is directed to apparatus and methods for rapid, automated, microscale sample analysis using pressure differentials. The invention includes an apparatus having intersecting channels for introduction of a sample and separation of that sample into its components. The sample introduction and separation channels preferably are etched in a microfabricated device, such as a microchip, to form a junction. Pressure gradients are applied to the channels to form a sample plug in the separation channel. The separation channel may have disposed within it a medium for separation of the components suspected to be contained in the sample. For example, with the proper medium, a voltage gradient may be applied along the separation channel to separate the components of the sample electrophoretically. The apparatus also may include means for detecting the components of the sample subsequent to separation.
摘要翻译：本发明涉及使用压力差的快速，自动化，微量样品分析的装置和方法。本发明包括具有用于引入样品和将样品分离成其组分的相交通道的装置。样品引入和分离通道优选在诸如微芯片的微制造装置中蚀刻以形成结。将压力梯度施加到通道以在分离通道中形成样品塞。分离通道可以在其内设置有用于分离怀疑包含在样品中的组分的介质。例如，使用适当的介质，可以沿着分离通道施加电压梯度以电泳分离样品的组分。该装置还可以包括用于在分离之后检测样品的组分的装置。

2. 发明申请

US20060144706A1 Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device 审中-公开
公开(公告)号：US20060144706A1
公开(公告)日：2006-07-06
申请号：US11300215
申请日：2005-12-13
申请人： Aram Adourian , Daniel Ehrlich , Lance Koutny , Paul Matsudaira , Dieter Schmalzing
发明人： Aram Adourian , Daniel Ehrlich , Lance Koutny , Paul Matsudaira , Dieter Schmalzing
IPC分类号： C07K1/26
CPC分类号： G01N27/44743 , B01L3/0275 , B01L3/0289 , B01L3/5025 , B01L3/5027 , B01L3/502715 , B01L3/502753 , B01L2200/027 , B01L2200/0605 , B01L2200/0647 , B01L2200/0657 , B01L2300/0803 , B01L2300/0816 , B01L2400/0415 , B01L2400/0421 , B01L2400/043 , B01L2400/0442 , G01N27/44782 , G01N35/10 , Y10T436/2575
摘要： A technique processes a sample of biomolecular analyte. The technique uses an apparatus having a support assembly that receives and supports a test module, a load assembly that loads the sample of biomolecular analyte onto the test module, an electrophoresis assembly that applies a current to the test module such that components within the sample separate by electrophoresis, and a controller that controls operations of the load assembly and the electrophoresis assembly. The load assembly and the electrophoresis assembly are coupled to the support assembly. The controller controls the operation of the load assembly in an automated manner. Preferably, the test module includes a dielectric plate member having an upper planar surface and a lower planar surface that is spaced apart from and coplanar with the upper planar surface. The dielectric plate member has at least one set of channels that includes an injection channel and a separation channel. The injection channel extends from the upper planar surface to the lower planar surface. The separation channel extends within the dielectric plate member in a plane parallel with the upper and lower planar surfaces and intersects the injection channel.

3. 发明授权

US5958202A Capillary electrophoresis enzyme immunoassay 失效
标题翻译：毛细管电泳酶免疫测定
公开(公告)号：US5958202A
公开(公告)日：1999-09-28
申请号：US792933
申请日：1997-01-22
申请人： Fred E. Regnier , Xian-Wei Yao , Todd A. Taylor , Martin Fuchs , Dieter Schmalzing , Lance Koutny , Wassim Nashabeh
发明人： Fred E. Regnier , Xian-Wei Yao , Todd A. Taylor , Martin Fuchs , Dieter Schmalzing , Lance Koutny , Wassim Nashabeh
IPC分类号： G01N33/561 , G01N27/447 , G01N33/537 , G01N33/543 , G01N33/78 , G01N27/26 , C12Q1/68 , G01N33/53
CPC分类号： G01N27/44747 , G01N27/44726 , G01N33/537 , G01N33/54306 , G01N33/54366 , G01N33/78
摘要： Disclosed are methods and apparatus for performing highly sensitive enzyme-amplified assays using channel electrophoresis of an enzyme having a biorecognition moiety. Combining a binding reaction of an analyte and an enzyme having a biorecognition moiety, and an electrophoretic separation which uses enzyme-amplified detection methodology, assay methods and apparatus are provided that offer simplicity, quantitative sensitivity and rapid analysis. Also disclosed are highly sensitive catalytically-amplified assays using a capillary electrophoresis format in which the binding reaction prior to electrophoresis is heterogeneous or homogeneous, and direct or competitive. The methods and apparatus for conducting sensitive automated catalytically-amplified assays using electrophoresis may be conducted in a microscale format.
摘要翻译：公开了使用具有生物识别部分的酶的通道电泳进行高度灵敏的酶扩增测定的方法和装置。结合分析物和具有生物识别部分的酶的结合反应以及使用酶扩增检测方法的测定方法和装置，提供了简单性，定量灵敏度和快速分析的电泳分离。还公开了使用毛细管电泳方式的高度敏感的催化扩增测定法，其中电泳前的结合反应是异质或均相的，直接的或竞争性的。使用电泳进行敏感的自动化催化扩增测定的方法和装置可以以微型格式进行。

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