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1. 发明授权

US08021653B2 Shuttle vector for Bifidobacterium and Escherichia coli 有权
标题翻译：穿梭载体为双歧杆菌和大肠杆菌
公开(公告)号：US08021653B2
公开(公告)日：2011-09-20
申请号：US11718680
申请日：2005-11-24
申请人： Yasunobu Kano , Yoshinori Hamaji , Minoru Fujimori , Takayuki Sasaki , Kyoko Kohno , Jun Amano , Shun'ichiro Taniguchi
发明人： Yasunobu Kano , Yoshinori Hamaji , Minoru Fujimori , Takayuki Sasaki , Kyoko Kohno , Jun Amano , Shun'ichiro Taniguchi
IPC分类号： C07H21/04 , C12N15/00 , C12N1/21
CPC分类号： C12N15/70 , A61K38/00 , C12N15/746
摘要： The present invention provides a shuttle vector for a microorganism of the genus Bifidobacterium (BM) and Escherichia coli having a wide host range and a large copy number in BM and capable of highly expressing a desired protein when used as an expression vector; an expression vector capable of expressing a desired gene in BM by use of the shuttle vector; BM transformed with the expression vector; and an antitumor agent comprising the BM as an active ingredient. It comprises a pTB6-derived region portion comprising a replication origin (oriV)-repB region of pTB6 but not comprising MembB, MobA, OrfI, and oriT regions of pTB6 and an Escherichia coli-derived plasmid portion comprising a replication origin (Puc Ori) region of Escherichia coli but having deleted DNA encoding an N-terminal region of an ampicillin resistance gene (ampR) expression product β-lactamase.
摘要翻译：本发明提供了一种用于双歧杆菌（BM）和微生物的穿梭载体，其具有宽的宿主范围，并且在BM中具有大的拷贝数，并且当用作表达载体时能够高度表达所需的蛋白质。能够通过穿梭载体在BM中表达所需基因的表达载体; BM用表达载体转化; 和包含BM作为活性成分的抗肿瘤剂。其包含pTB6衍生的区域部分，其包含pTB6的复制起点（oriV）-repB区，但不包含pBB6的MembB，MobA，OrfI和oriT区以及包含复制起点（Puc Ori）的大肠杆菌来源的质粒部分，大肠杆菌的区域，但是具有编码氨苄青霉素抗性基因（ampR）表达产物＆amp;内酰胺酶的N末端区域的缺失的DNA。

2. 发明申请

US20100129323A1 Novel Shuttle Vector 有权
标题翻译：小号班车矢量
公开(公告)号：US20100129323A1
公开(公告)日：2010-05-27
申请号：US11718680
申请日：2005-11-24
申请人： Yasunobu Kano , Yoshinori Hamaji , Minoru Fujimori , Takayuki Sasaki , Kyoko Kohno , Jun Amano , Shun'ichiro Taniguchi
发明人： Yasunobu Kano , Yoshinori Hamaji , Minoru Fujimori , Takayuki Sasaki , Kyoko Kohno , Jun Amano , Shun'ichiro Taniguchi
IPC分类号： A61K35/00 , A61P43/00 , C12N1/20 , C12N15/00
CPC分类号： C12N15/70 , A61K38/00 , C12N15/746
摘要： The present invention provides a shuttle vector for a microorganism of the genus Bifidobacterium (BM) and Escherichia coli having a wide host range and a large copy number in BM and capable of highly expressing a desired protein when used as an expression vector; an expression vector capable of expressing a desired gene in BM by use of the shuttle vector; BM transformed with the expression vector; and an antitumor agent comprising the BM as an active ingredient. It comprises a pTB6-derived region portion comprising a replication origin (oriV)-repB region of pTB6 but not comprising MembB, MobA, OrfI, and oriT regions of pTB6 and an Escherichia coli-derived plasmid portion comprising a replication origin (oriC) region of Escherichia coli but having deleted DNA encoding an N-terminal region of an ampicillin resistance gene (ampR) expression product β-lactamase, and constitute a shuttle vector for Escherichia coli with a BM having an average copy number of 6 to 30.
摘要翻译：本发明提供了一种用于双歧杆菌（BM）和微生物的穿梭载体，其具有宽的宿主范围，并且在BM中具有大的拷贝数，并且当用作表达载体时能够高度表达所需的蛋白质。能够通过穿梭载体在BM中表达所需基因的表达载体; BM用表达载体转化; 以及含有BM作为活性成分的抗肿瘤剂。其包含pTB6衍生的区域部分，其包含pTB6的复制起点（oriV）-repB区，但不包含pBB6的MembB，MobA，OrfI和oriT区以及包含复制起点（oriC）区的大肠杆菌来源的质粒部分的大肠杆菌，但是具有编码氨苄青霉素抗性基因（ampR）表达产物和β-内酰胺酶的N末端区域的缺失的DNA，并且构建具有平均拷贝数为6至30的BM的大肠杆菌的穿梭载体。

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