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1. 发明授权

US07407774B2 Method for reducing influence of hemoglobin with albumin in an assay 有权
标题翻译：在测定中减少血红蛋白与白蛋白的影响的方法
公开(公告)号：US07407774B2
公开(公告)日：2008-08-05
申请号：US11684827
申请日：2007-03-12
申请人： Hiroyuki Ebinuma , Kumiko Yuki
发明人： Hiroyuki Ebinuma , Kumiko Yuki
IPC分类号： C12Q1/26
CPC分类号： C12Q1/32
摘要： A method for quantitatively determining a specific component in a biological specimen, which includes reacting a biological specimen, in the presence of an electron acceptor, with an enzyme which has an ability, by the dehydrogenation reaction, to oxidize the specific component or a substance derived from the specific component, and measuring the formed reductant of the electron acceptor, wherein the method avoids the influence of hemoglobin effectively if any contained in the specimen by using a measuring reagent containing albumin, thereby making an quantitative determination of the target component accurately; and a reagent for the quantitative determination.
摘要翻译：一种用于定量测定生物样本中的特定成分的方法，其包括使生物样品在电子受体存在下与通过脱氢反应具有能够氧化特定成分或衍生物质的酶反应的酶测定形成的电子受体的还原剂，其中，通过使用含有白蛋白的测定试剂，如果含有试样中的任何含量，则可有效地避免血红蛋白的影响，从而准确地定量确定目标成分; 和用于定量测定的试剂。

2. 发明申请

US20130244314A1 IMMUNOCHROMATOGRAPHIC TEST STRIP AND MANUFACTURING METHOD THEREOF 有权
标题翻译：免疫荧光测试条及其制造方法
公开(公告)号：US20130244314A1
公开(公告)日：2013-09-19
申请号：US13877024
申请日：2011-09-29
申请人： Kumiko Yuki , Yuka Sasaki
发明人： Kumiko Yuki , Yuka Sasaki
IPC分类号： G01N33/569
CPC分类号： G01N33/56983 , G01N33/54386 , G01N33/558 , G01N2333/11
摘要： An immunochromatographic test strip that achieves a shorter reaction completion time and excellent sensitivity is provided. The immunochromatographic test strip comprises: (1) a conjugate pad comprising a sample-supplying section supplying a sample possibly containing an analyte and a line-shaped conjugate section containing a conjugate in which an antibody or antigen immunologically reactive with the analyte is immobilized to a label on the downstream side relative to the sample-supplying section; and (2) an insoluble membrane support having at least one detecting section to which an antibody or antigen immunologically reactive with the analyte is immobilized, the lower surface of the sample-supplying section of the conjugate pad being not in contact with the upper surface of the insoluble membrane support, the lower surface of the conjugate section of the conjugate pad being in contact with the upper surface of the insoluble membrane support.
摘要翻译：提供了实现更短的反应完成时间和优异的灵敏度的免疫色谱测试条。免疫色谱测试条包括：（1）包含供应可能含有分析物的样品的样品供应部分和含有其中将与分析物免疫反应的抗体或抗原固定在其上的缀合物的线形缀合物部分的缀合物垫标签在下游侧相对于样品供应部分; 和（2）具有至少一个检测部分的不溶性膜支持体，其中固定有与分析物免疫反应的抗体或抗原，所述缀合物垫的样品供应部分的下表面不与不溶性膜载体，共轭垫的共轭部分的下表面与不溶性膜载体的上表面接触。

3. 发明授权

US07759072B2 Immunoassay method and reagent therefor 有权
标题翻译：免疫测定法及其试剂
公开(公告)号：US07759072B2
公开(公告)日：2010-07-20
申请号：US11817515
申请日：2006-03-02
申请人： Koji Ushizawa , Michiko Kawamoto , Shoko Yamamoto , Kumiko Yuki , Yoko Ikeda , Mitsuaki Yamamoto
发明人： Koji Ushizawa , Michiko Kawamoto , Shoko Yamamoto , Kumiko Yuki , Yoko Ikeda , Mitsuaki Yamamoto
IPC分类号： G01N33/53
CPC分类号： G01N33/581 , G01N33/5306 , G01N33/542 , G01N33/54393
摘要： The present invention provides a novel immunoassay method with high reaction specificity and high sensitivity. The present invention also provides a method for immunoassaying a target antigen utilizing reactivation of an apoenzyme, which includes simultaneously or sequentially adding a test sample to an antibody specific to the target antigen, the target antigen labeled with a coenzyme, an apo-D-amino acid oxidase, a D-amino acid, and a reagent for detecting a hydrogen peroxide produced by the oxidase.
摘要翻译：本发明提供了具有高反应特异性和高灵敏度的新型免疫测定方法。本发明还提供了使用辅酶的再活化来免疫测定靶抗原的方法，其包括同时或依次将测试样品加入到靶抗原特异性抗体中，用辅酶标记的靶抗原，载脂蛋白-D-氨基酸性氧化酶，D-氨基酸和用于检测由氧化酶产生的过氧化氢的试剂。

4. 发明申请

US20070154879A1 METHOD FOR QUANTITATIVELY DETERMINING A SPECIFIC COMPONENT IN A BIOLOGICAL SPECIMEN, AND REAGENT FOR QUANTITATIVE DETERMINATION 有权
标题翻译：定量测定生物样本中特定成分的方法和定量测定试剂
公开(公告)号：US20070154879A1
公开(公告)日：2007-07-05
申请号：US11684827
申请日：2007-03-12
申请人： Hiroyuki Ebinuma , Kumiko Yuki
发明人： Hiroyuki Ebinuma , Kumiko Yuki
IPC分类号： C12Q1/00 , C12Q1/26
CPC分类号： C12Q1/32
摘要： A method for quantitatively determining a specific component in a biological specimen, which includes reacting a biological specimen, in the presence of an electron acceptor, with an enzyme which has an ability, by the dehydrogenation reaction, to oxidize the specific component or a substance derived from the specific component, and measuring the formed reductant of the electron acceptor, wherein the method avoids the influence of hemoglobin effectively if any contained in the specimen by using a measuring reagent containing albumin, thereby making an quantitative determination of the target component accurately; and a reagent for the quantitative determination.
摘要翻译：一种用于定量测定生物样本中的特定成分的方法，其包括使生物样品在电子受体存在下与通过脱氢反应具有能够氧化特定成分或衍生物质的酶反应的酶测定形成的电子受体的还原剂，其中，通过使用含有白蛋白的测定试剂，如果含有试样中的任何含量，则可有效地避免血红蛋白的影响，从而准确地定量确定目标成分; 和用于定量测定的试剂。

5. 发明申请

US20050164330A1 Method for quantitatively determining a specific component in a biological specimen, and reagent for quantitative determination 审中-公开
标题翻译：用于定量测定生物样本中特定组分的方法和用于定量测定的试剂
公开(公告)号：US20050164330A1
公开(公告)日：2005-07-28
申请号：US10764455
申请日：2004-01-27
申请人： Hiroyuki Ebinuma , Kumiko Yuki
发明人： Hiroyuki Ebinuma , Kumiko Yuki
IPC分类号： C12Q1/26 , C12Q1/32
CPC分类号： C12Q1/32
摘要： A method for quantitatively determining a specific component in a biological specimen, which includes reacting a biological specimen, in the presence of an electron acceptor, with an enzyme which has an ability, by the dehydrogenation reaction, to oxidize the specific component or a substance derived from the specific component, and measuring the formed reductant of the electron acceptor, wherein the method avoids the influence of hemoglobin effectively if any contained in the specimen by using a measuring reagent containing albumin, thereby making an quantitative determination of the target component accurately; and a reagent for the quantitative determination.
摘要翻译：一种用于定量测定生物样本中的特定成分的方法，其包括使生物样品在电子受体存在下与通过脱氢反应具有能够氧化特定成分或衍生物质的酶反应的酶测定形成的电子受体的还原剂，其中，通过使用含有白蛋白的测定试剂，如果含有试样中的任何含量，则可有效地避免血红蛋白的影响，从而准确地定量确定目标成分; 和用于定量测定的试剂。

6. 发明授权

US09435806B2 Immunochromatographic test strip and manufacturing method thereof 有权
标题翻译：免疫层析试片及其制造方法
公开(公告)号：US09435806B2
公开(公告)日：2016-09-06
申请号：US13877024
申请日：2011-09-29
申请人： Kumiko Yuki , Yuka Sasaki
发明人： Kumiko Yuki , Yuka Sasaki
IPC分类号： G01N33/558 , G01N33/569 , G01N33/543
CPC分类号： G01N33/56983 , G01N33/54386 , G01N33/558 , G01N2333/11
摘要： An immunochromatographic test strip that achieves a shorter reaction completion time and excellent sensitivity is provided. The immunochromatographic test strip comprises: (1) a conjugate pad comprising a sample-supplying section supplying a sample possibly containing an analyte and a line-shaped conjugate section containing a conjugate in which an antibody or antigen immunologically reactive with the analyte is immobilized to a label on the downstream side relative to the sample-supplying section; and (2) an insoluble membrane support having at least one detecting section to which an antibody or antigen immunologically reactive with the analyte is immobilized, the lower surface of the sample-supplying section of the conjugate pad being not in contact with the upper surface of the insoluble membrane support, the lower surface of the conjugate section of the conjugate pad being in contact with the upper surface of the insoluble membrane support.
摘要翻译：提供了实现更短的反应完成时间和优异的灵敏度的免疫色谱测试条。免疫色谱测试条包括：（1）包含供应可能含有分析物的样品的样品供应部分和含有其中将与分析物免疫反应的抗体或抗原固定在其上的缀合物的线形缀合物部分的缀合物垫标签在下游侧相对于样品供应部分; 和（2）具有至少一个检测部分的不溶性膜支持体，其中固定有与分析物免疫反应的抗体或抗原，所述缀合物垫的样品供应部分的下表面不与不溶性膜载体，共轭垫的共轭部分的下表面与不溶性膜载体的上表面接触。

7. 发明申请

US20090035785A1 IMMUNOASSAY METHOD AND REAGENT THEREFOR 有权
标题翻译：免疫方法和试剂
公开(公告)号：US20090035785A1
公开(公告)日：2009-02-05
申请号：US11817515
申请日：2006-03-02
申请人： Koji Ushizawa , Michiko Kawamoto , Shoko Yamamoto , Kumiko Yuki , Yoko Ikeda , Mitsuaki Yamamoto
发明人： Koji Ushizawa , Michiko Kawamoto , Shoko Yamamoto , Kumiko Yuki , Yoko Ikeda , Mitsuaki Yamamoto
IPC分类号： G01N33/53
CPC分类号： G01N33/581 , G01N33/5306 , G01N33/542 , G01N33/54393
摘要： The present invention provides a novel immunoassay method with high reaction specificity and high sensitivity. The present invention also provides a method for immunoassaying a target antigen utilizing reactivation of an apoenzyme, which includes simultaneously or sequentially adding a test sample to an antibody specific to the target antigen, the target antigen labeled with a coenzyme, an apo-D-amino acid oxidase, a D-amino acid, and a reagent for detecting a hydrogen peroxide produced by the oxidase.
摘要翻译：本发明提供了具有高反应特异性和高灵敏度的新型免疫测定方法。本发明还提供了使用辅酶的再活化来免疫测定靶抗原的方法，其包括同时或依次将测试样品加入到靶抗原特异性抗体中，用辅酶标记的靶抗原，载脂蛋白-D-氨基酸性氧化酶，D-氨基酸和用于检测由氧化酶产生的过氧化氢的试剂。

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