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    • 2. 发明申请
    • WHOLE GENOME AMPLIFICATION METHOD AND APPLICATION THEREOF
    • 全基因扩增方法及其应用
    • US20150299753A1
    • 2015-10-22
    • US14378935
    • 2012-03-30
    • Kui WUHanjie WUYong HOUXun XUJun WANG
    • Kui WUHanjie WUYong HOUXun XUJun WANG
    • C12P19/34C12Q1/68
    • C12P19/34C12Q1/6806C12Q1/686C12Q2537/149
    • Provided are a whole genome sample amplification method, a whole genome sequencing method, and a method for determining whether an abnormal state occurs in a whole genome, a whole genome sample amplification apparatus, a whole genome sequencing device, and a system for determining whether an abnormal state occurs in a whole genome. The whole genome sample amplification method comprises: subjecting a whole genome sample to a first amplification reaction, so as to obtain a first amplification product; and subjecting the first amplification product to a second amplification reaction, so as to obtain a second amplification product. The first amplification reaction is one of the PCR-based amplification reaction and the isothermal amplification reaction, and the second amplification reaction is the other of the PCR-based amplification reaction and the isothermal amplification reaction.
    • 提供了全基因组样品扩增方法,全基因组测序方法以及用于确定全基因组中是否发生异常状态的方法,全基因组样品扩增装置,全基因组测序装置和用于确定是否 异常状态发生在整个基因组中。 全基因组样品扩增方法包括:对全基因组样品进行第一次扩增反应,得到第一扩增产物; 并对第一扩增产物进行第二次扩增反应,得到第二扩增产物。 第一次扩增反应是基于PCR的扩增反应和等温扩增反应之一,第二次扩增反应是基于PCR的扩增反应和等温扩增反应中的另一个。
    • 6. 发明申请
    • Method and device for genetic map construction, method and device for haplotype analysis
    • 用于遗传图谱构建的方法和装置,用于单倍型分析的方法和装置
    • US20140194300A1
    • 2014-07-10
    • US14240955
    • 2012-08-24
    • Luting SongDi ShaoZequn ZhengZhijun ZhengKui WuShuheng LiangYe TaoYong Hou
    • Luting SongDi ShaoZequn ZhengZhijun ZhengKui WuShuheng LiangYe TaoYong Hou
    • C12Q1/68
    • C12Q1/6869C12Q1/6874C12Q1/6881C12Q2600/172G06F19/20G06F19/22
    • Provided are the method and device for genetic map construction and the method and device for haplotype determination of a single cell. Wherein the method for genetic map construction includes: whole genome sequencing for at least a single cell from a same species, aligning the sequencing data to reference sequences respectively to determine genotypes of SNP sites, determining male parent a/female parent b typing results of SNP genotypes of a single cell based on the genotypes of SNP sites, dividing the chromosome of the species into linkage regions based on the male parent a/female parent b typing results of SNP genotypes, determining the variation ratio of a/b between two linkage regions to obtain recombination rate between every two continuous linkage regions, determining recombination map of a single cell according to the recombination rate, wherein the boundary site of a and b is the recombination site, determining the recombination rate of each recombination rate based on the recombination map to construct a genetic map of the species.
    • 提供遗传图谱构建的方法和装置以及单细胞单体型确定的方法和装置。 遗传图谱构建方法包括:对同一物种的至少一个细胞进行全基因组测序,分别将测序数据与参照序列进行比对,以确定SNP位点的基因型,确定SNP的雄性亲本a /雌性亲本B分型结果 基于SNP位点基因型的单细胞基因型,根据SNP基因型的雄性亲本a /雌性亲本B分型结果将该物种的染色体分为连锁区,确定两个连锁区之间a / b的变异比 以获得每两个连续连锁区域之间的重组率,根据复合率确定单个细胞的重组图谱,其中a和b的边界位点是重组位点,基于重组图确定每个重组速率的重组率 构建该物种的遗传图谱。
    • 7. 发明授权
    • Method and device for genetic map construction, method and device for haplotype analysis
    • 用于遗传图谱构建的方法和装置,用于单倍型分析的方法和装置
    • US09309570B2
    • 2016-04-12
    • US14240955
    • 2012-08-24
    • Luting SongDi ShaoZequn ZhengZhijun ZhengKui WuShuheng LiangYe TaoYong Hou
    • Luting SongDi ShaoZequn ZhengZhijun ZhengKui WuShuheng LiangYe TaoYong Hou
    • C12Q1/68C12M1/34G01N33/48G06F19/20G06F19/22
    • C12Q1/6869C12Q1/6874C12Q1/6881C12Q2600/172G06F19/20G06F19/22
    • Provided are the method and device for genetic map construction and the method and device for haplotype determination of a single cell. Wherein the method for genetic map construction includes: whole genome sequencing for at least a single cell from a same species, aligning the sequencing data to reference sequences respectively to determine genotypes of SNP sites, determining male parent a/female parent b typing results of SNP genotypes of a single cell based on the genotypes of SNP sites, dividing the chromosome of the species into linkage regions based on the male parent a/female parent b typing results of SNP genotypes, determining the variation ratio of a/b between two linkage regions to obtain recombination rate between every two continuous linkage regions, determining recombination map of a single cell according to the recombination rate, wherein the boundary site of a and b is the recombination site, determining the recombination rate of each recombination rate based on the recombination map to construct a genetic map of the species.
    • 提供遗传图谱构建的方法和装置以及单细胞单体型确定的方法和装置。 遗传图谱构建方法包括:对同一物种的至少一个细胞进行全基因组测序,分别将测序数据与参照序列进行比对,以确定SNP位点的基因型,确定SNP的雄性亲本a /雌性亲本B分型结果 基于SNP位点基因型的单细胞基因型,根据SNP基因型的雄性亲本a /雌性亲本B分型结果将该物种的染色体分为连锁区,确定两个连锁区之间a / b的变异比 以获得每两个连续连锁区域之间的重组率,根据复合率确定单个细胞的重组图谱,其中a和b的边界位点是重组位点,基于重组图确定每个重组速率的重组率 构建该物种的遗传图谱。