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1. 发明授权

US08541219B2 High fidelity reverse transcriptases and the uses thereof 有权
标题翻译：高保真逆转录酶及其用途
公开(公告)号：US08541219B2
公开(公告)日：2013-09-24
申请号：US12765253
申请日：2010-04-22
申请人： Robert Potter , Kim Rosenthal
发明人： Robert Potter , Kim Rosenthal
IPC分类号： C12N9/00 , C12N9/12 , C07K1/00 , C07K14/00 , C07K17/00 , C12Q1/68 , C12P19/34
CPC分类号： C12N9/1276 , C12Q2521/107
摘要： The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.
摘要翻译：本发明涉及具有增加的保真度（或降低的错配率）和/或末端脱氧核苷酸转移酶活性的逆转录酶。特别地，本发明涉及通过修饰或突变逆转录酶中的指定位置来制备此类逆转录酶的方法。本发明还涉及含有编码本发明的逆转录酶的基因的宿主细胞含有此类核酸分子的核酸分子以及使用宿主细胞制备逆转录酶的方法。本发明的逆转录酶特别适用于核酸合成，测序，扩增和cDNA合成。

2. 发明申请

US20060094050A1 High fidelity reverse transcriptases and uses thereof 审中-公开
公开(公告)号：US20060094050A1
公开(公告)日：2006-05-04
申请号：US11265159
申请日：2005-11-03
申请人： Robert Potter , Kim Rosenthal
发明人： Robert Potter , Kim Rosenthal
IPC分类号： C12Q1/68 , C12N9/22
CPC分类号： C12N9/1276 , C12Q2521/107
摘要： The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.

3. 发明申请

US20070141592A1 Thermostable reverse transcriptases and uses thereof 审中-公开
标题翻译：热稳定逆转录酶及其用途
公开(公告)号：US20070141592A1
公开(公告)日：2007-06-21
申请号：US11492194
申请日：2006-07-25
申请人： Michael Smith , Robert Potter , Gulshan Dhariwal , Gary Gerard , Kim Rosenthal , Jun Lee
发明人： Michael Smith , Robert Potter , Gulshan Dhariwal , Gary Gerard , Kim Rosenthal , Jun Lee
IPC分类号： C12Q1/68 , C07H21/04 , C12P21/06 , C12N9/22 , C12N15/86
CPC分类号： C12N9/1276 , C12P19/34 , C12Y207/07049
摘要： The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.
摘要翻译：本发明涉及分子和细胞生物学领域。本发明通常涉及逆转录酶和逆转录核酸分子，特别是信使RNA分子的方法。具体地，本发明涉及已被突变或修饰以增加热稳定性，降低末端脱氧核苷酸转移酶活性和/或增加保真度的逆转录酶，以及使用这些方法来产生，扩增或测序核酸分子（特别是cDNA分子）的方法逆转录酶或组合物。本发明还涉及通过这些方法产生的核酸分子以及这种核酸分子用于产生所需多肽的用途。本发明还涉及包含这些酶或组合物的试剂盒。

4. 发明授权

US08753845B2 Thermostable reverse transcriptases and uses thereof 有权
标题翻译：热稳定逆转录酶及其用途
公开(公告)号：US08753845B2
公开(公告)日：2014-06-17
申请号：US12979899
申请日：2010-12-28
申请人： Gulshan Dhariwal , Gary Gerard , Jun Lee , Robert Potter , Kim Rosenthal , Michael D. Smith
发明人： Gulshan Dhariwal , Gary Gerard , Jun Lee , Robert Potter , Kim Rosenthal , Michael D. Smith
IPC分类号： C12P19/34 , C12N9/12
CPC分类号： C12N9/1276 , C12P19/34 , C12Y207/07049
摘要： The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.
摘要翻译：本发明涉及分子和细胞生物学领域。本发明通常涉及逆转录酶和逆转录核酸分子，特别是信使RNA分子的方法。具体地，本发明涉及已被突变或修饰以增加热稳定性，降低末端脱氧核苷酸转移酶活性和/或增加保真度的逆转录酶，以及使用这些方法来产生，扩增或测序核酸分子（特别是cDNA分子）的方法逆转录酶或组合物。本发明还涉及通过这些方法产生的核酸分子以及这种核酸分子用于产生所需多肽的用途。本发明还涉及包含这些酶或组合物的试剂盒。

5. 发明申请

US20110165651A1 HIGH FIDELITY REVERSE TRANSCRIPTASES AND THE USES THEREOF 有权
标题翻译：高度反转的转录物及其用途
公开(公告)号：US20110165651A1
公开(公告)日：2011-07-07
申请号：US12765253
申请日：2010-04-22
申请人： Robert Jason Potter , Kim Rosenthal
发明人： Robert Jason Potter , Kim Rosenthal
IPC分类号： C12N9/12
CPC分类号： C12N9/1276 , C12Q2521/107
摘要： The invention relates to reverse transcriptases which have increased fidelity (or reduced misincorporation rate) and/or terminal deoxynucleotidyl transferase activity. In particular, the invention relates to a method of making such reverse transcriptases by modifying or mutating specified positions in the reverse transcriptases. The invention also relates to nucleic acid molecules containing the genes encoding the reverse trancriptases of the invention, to host cells containing such nucleic acid molecules and to methods to make the reverse trancriptases using the host cells. The reverse transcriptases of the invention are particularly suited for nucleic acid synthesis, sequencing, amplification and cDNA synthesis.
摘要翻译：本发明涉及具有增加的保真度（或降低的错配率）和/或末端脱氧核苷酸转移酶活性的逆转录酶。特别地，本发明涉及通过修饰或突变逆转录酶中的指定位置来制备此类逆转录酶的方法。本发明还涉及含有编码本发明的逆转录酶的基因的宿主细胞含有此类核酸分子的核酸分子以及使用宿主细胞制备逆转录酶的方法。本发明的逆转录酶特别适用于核酸合成，测序，扩增和cDNA合成。

6. 发明申请

US20110081704A1 THERMOSTABLE REVERSE TRANSCRIPTASES AND USES THEREOF 有权
标题翻译：可逆转的转录物及其用途
公开(公告)号：US20110081704A1
公开(公告)日：2011-04-07
申请号：US12861797
申请日：2010-08-23
申请人： Michael D. Smith , Robert Jason Potter , Gulshan Dhariwal , Gary F. Gerard , Kim Rosenthal
发明人： Michael D. Smith , Robert Jason Potter , Gulshan Dhariwal , Gary F. Gerard , Kim Rosenthal
IPC分类号： C12N9/12
CPC分类号： C12N9/1276
摘要： The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.
摘要翻译：本发明涉及分子和细胞生物学领域。本发明通常涉及逆转录酶和逆转录核酸分子，特别是信使RNA分子的方法。具体地，本发明涉及已被突变或修饰以增加热稳定性，降低末端脱氧核苷酸转移酶活性和/或增加保真度的逆转录酶，以及使用这些方法来产生，扩增或测序核酸分子（特别是cDNA分子）的方法逆转录酶或组合物。本发明还涉及通过这些方法产生的核酸分子以及这种核酸分子用于产生所需多肽的用途。本发明还涉及包含这些酶或组合物的试剂盒。

7. 发明申请

US20100015683A1 THERMOSTABLE REVERSE TRANSCRIPTASES AND USES THEREOF 审中-公开
标题翻译：可逆转的转录物及其用途
公开(公告)号：US20100015683A1
公开(公告)日：2010-01-21
申请号：US12553061
申请日：2009-09-02
申请人： Michael D. Smith , Robert Jason Potter , Gulsham Dhariwal , Gary F. Gerard , Kim Rosenthal
发明人： Michael D. Smith , Robert Jason Potter , Gulsham Dhariwal , Gary F. Gerard , Kim Rosenthal
IPC分类号： C12N9/00
CPC分类号： C12N9/1276
摘要： The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.
摘要翻译：本发明涉及分子和细胞生物学领域。本发明通常涉及逆转录酶和逆转录核酸分子，特别是信使RNA分子的方法。具体地，本发明涉及已被突变或修饰以增加热稳定性，降低末端脱氧核苷酸转移酶活性和/或增加保真度的逆转录酶，以及使用这些方法来产生，扩增或测序核酸分子（特别是cDNA分子）的方法逆转录酶或组合物。本发明还涉及通过这些方法产生的核酸分子以及这种核酸分子用于产生所需多肽的用途。本发明还涉及包含这些酶或组合物的试剂盒。

8. 发明申请

US20120021464A1 THERMOSTABLE REVERSE TRANSCRIPTASES AND USES THEREOF 有权
标题翻译：可逆转的转录物及其用途
公开(公告)号：US20120021464A1
公开(公告)日：2012-01-26
申请号：US12979899
申请日：2010-12-28
申请人： Michael D. SMITH , Robert Jason POTTER , Gulshan DHARIWAL , Gary F. GERARD , Kim ROSENTHAL , Jun E. LEE
发明人： Michael D. SMITH , Robert Jason POTTER , Gulshan DHARIWAL , Gary F. GERARD , Kim ROSENTHAL , Jun E. LEE
IPC分类号： C12P19/34 , C12N9/12
CPC分类号： C12N9/1276 , C12P19/34 , C12Y207/07049
摘要： The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.
摘要翻译：本发明涉及分子和细胞生物学领域。本发明通常涉及逆转录酶和逆转录核酸分子，特别是信使RNA分子的方法。具体地，本发明涉及已被突变或修饰以增加热稳定性，降低末端脱氧核苷酸转移酶活性和/或增加保真度的逆转录酶，以及使用这些方法来产生，扩增或测序核酸分子（特别是cDNA分子）的方法逆转录酶或组合物。本发明还涉及通过这些方法产生的核酸分子以及这种核酸分子用于产生所需多肽的用途。本发明还涉及包含这些酶或组合物的试剂盒。

9. 发明申请

US20060211103A1 Thermostable reverse transcriptases and uses thereof 审中-公开
公开(公告)号：US20060211103A1
公开(公告)日：2006-09-21
申请号：US11437681
申请日：2006-05-22
申请人： Michael Smith , Robert Potter , Gulshan Dhariwal , Gary Gerard , Kim Rosenthal
发明人： Michael Smith , Robert Potter , Gulshan Dhariwal , Gary Gerard , Kim Rosenthal
IPC分类号： C12N9/22
CPC分类号： C12N9/1276
摘要： The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

10. 发明授权

US07078208B2 Thermostable reverse transcriptases and uses thereof 有权
公开(公告)号：US07078208B2
公开(公告)日：2006-07-18
申请号：US09845157
申请日：2001-05-01
申请人： Michael D. Smith , Robert Jason Potter , Gulshan Dhariwal , Gary F. Gerard , Kim Rosenthal
发明人： Michael D. Smith , Robert Jason Potter , Gulshan Dhariwal , Gary F. Gerard , Kim Rosenthal
IPC分类号： C12N9/12
CPC分类号： C12N9/1276
摘要： The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

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