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1. 发明申请

US20150305341A1 COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS 审中-公开
标题翻译：组合物及其控制方法
公开(公告)号：US20150305341A1
公开(公告)日：2015-10-29
申请号：US14793893
申请日：2015-07-08
申请人： Karen E. Broglie , Kevin Kriss , Albert L. Lu , Mani Muthalagi , James Kevin Presnail
发明人： Karen E. Broglie , Kevin Kriss , Albert L. Lu , Mani Muthalagi , James Kevin Presnail
IPC分类号： A01N57/16 , A01N65/20 , A01N65/44 , C12N15/82 , C12N15/113
CPC分类号： A01N57/16 , A01N63/00 , A01N65/20 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N15/8286 , C12N2310/11 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barberi, D. speciosa, or D. undecimpunctata howardi. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当由有害生物摄取的诸如鞘翅目植物病虫害（Diaprotica plant pest）的害虫时，会降低害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了SEQ ID NO：1-236中任一项所述的各种靶多核苷酸或其活性变体及其片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性活动）。进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是D. virgifera virgifera，D.barberi，D. speciosa或D. undecimpunctata howardi。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

2. 发明申请

US20110054007A1 COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS 审中-公开
标题翻译：组合物及其控制方法
公开(公告)号：US20110054007A1
公开(公告)日：2011-03-03
申请号：US12868994
申请日：2010-08-26
申请人： Karen E. Broglie , Kevin Kriss , Albert Laurence Lu , Mani Muthalagi , James K. Presnail
发明人： Karen E. Broglie , Kevin Kriss , Albert Laurence Lu , Mani Muthalagi , James K. Presnail
IPC分类号： A01N57/16 , C07H21/04 , C07H21/02 , A01H5/00 , A01H5/10 , A01P7/04 , C12N5/10 , C12N15/63
CPC分类号： A01N57/16 , A01N63/00 , A01N65/20 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N15/8286 , C12N2310/11 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barberi, D. speciosa, or D. undecimpunctata howardi. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当由有害生物摄取的诸如鞘翅目植物病虫害（Diaprotica plant pest）的害虫时，会降低害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了SEQ ID NO：1-236中任一项所述的各种靶多核苷酸或其活性变体及其片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性活动）。进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是D. virgifera virgifera，D.barberi，D. speciosa或D. undecimpunctata howardi。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

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