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1. 发明授权

US08229196B2 Method of detecting specific polymer crystal 有权
标题翻译：检测特定聚合物晶体的方法
公开(公告)号：US08229196B2
公开(公告)日：2012-07-24
申请号：US13006883
申请日：2011-01-14
申请人： Akihito Yamano , Takahisa Sato , Hiroki Yoshida , Motohide Yoshimura , Kensaku Hamada
发明人： Akihito Yamano , Takahisa Sato , Hiroki Yoshida , Motohide Yoshimura , Kensaku Hamada
IPC分类号： G06K9/00
CPC分类号： G01N21/6486 , G01N21/6452 , G01N21/6458
摘要： In a specific macromolecule crystal detecting method according to the present invention, ultraviolet light is irradiated to sample solution, and a fluorescent image emitted from a sample in the sample solution is detected to detect specific macromolecules in the sample solution. Furthermore, by detecting the outline of the sample from the visible light image of the sample contained in the sample solution, the crystal is discriminated from other materials on the basis of the outline. By integrating the detection results of the fluorescent image and the visible light image, the specific macromolecule crystal is detected from the sample solution.
摘要翻译：在根据本发明的特定的大分子晶体检测方法中，对样品溶液照射紫外光，并且检测从样品溶液中的样品发射的荧光图像，以检测样品溶液中的特定大分子。此外，通过从包含在样品溶液中的样品的可见光图像中检测样品的轮廓，基于轮廓来鉴别晶体与其它材料。通过整合荧光图像和可见光图像的检测结果，从样品溶液中检测特定的大分子晶体。

2. 发明申请

US20060222220A1 Method for detecting specified polymer crystal 有权
标题翻译：检测指定聚合物晶体的方法
公开(公告)号：US20060222220A1
公开(公告)日：2006-10-05
申请号：US10569044
申请日：2004-08-18
申请人： Akihito Yamano , Takahisa Sato , Hiroki Yoshida , Motohide Yoshimura , Kensaku Hamada
发明人： Akihito Yamano , Takahisa Sato , Hiroki Yoshida , Motohide Yoshimura , Kensaku Hamada
IPC分类号： G06K9/00
CPC分类号： G01N21/6452 , G01N21/6458 , G01N21/6486
摘要： In a specific macromolecule crystal detecting method according to the present invention, ultraviolet light is irradiated to sample solution, and a fluorescent image emitted from a sample in the sample solution is detected to detect specific macromolecules in the sample solution. Furthermore, by detecting the outline of the sample from the visible light image of the sample contained in the sample solution, the crystal is discriminated from other materials on the basis of the outline. By integrating the detection results of the fluorescent image and the visible light image, the specific macromolecule crystal is detected from the sample solution.
摘要翻译：在根据本发明的特定的大分子晶体检测方法中，对样品溶液照射紫外光，并且检测从样品溶液中的样品发射的荧光图像，以检测样品溶液中的特定大分子。此外，通过从包含在样品溶液中的样品的可见光图像中检测样品的轮廓，基于轮廓来鉴别晶体与其它材料。通过整合荧光图像和可见光图像的检测结果，从样品溶液中检测特定的大分子晶体。

3. 发明授权

US08041086B2 Method of detecting specific polymer crystal 有权
标题翻译：检测特定聚合物晶体的方法
公开(公告)号：US08041086B2
公开(公告)日：2011-10-18
申请号：US10569044
申请日：2004-08-18
申请人： Akihito Yamano , Takahisa Sato , Hiroki Yoshida , Motohide Yoshimura , Kensaku Hamada
发明人： Akihito Yamano , Takahisa Sato , Hiroki Yoshida , Motohide Yoshimura , Kensaku Hamada
IPC分类号： G06K9/00
CPC分类号： G01N21/6452 , G01N21/6458 , G01N21/6486
摘要： In a specific macromolecule crystal detecting method according to the present invention, ultraviolet light is irradiated to sample solution, and a fluorescent image emitted from a sample in the sample solution is detected to detect specific macromolecules in the sample solution. Furthermore, by detecting the outline of the sample from the visible light image of the sample contained in the sample solution, the crystal is discriminated from other materials on the basis of the outline. By integrating the detection results of the fluorescent image and the visible light image, the specific macromolecule crystal is detected from the sample solution.
摘要翻译：在根据本发明的特定的大分子晶体检测方法中，对样品溶液照射紫外光，并且检测从样品溶液中的样品发射的荧光图像，以检测样品溶液中的特定大分子。此外，通过从包含在样品溶液中的样品的可见光图像中检测样品的轮廓，基于轮廓来鉴别晶体与其它材料。通过整合荧光图像和可见光图像的检测结果，从样品溶液中检测特定的大分子晶体。

4. 发明授权

US07342995B2 Apparatus for estimating specific polymer crystal 失效
标题翻译：用于估计特定聚合物晶体的装置
公开(公告)号：US07342995B2
公开(公告)日：2008-03-11
申请号：US10568740
申请日：2004-08-18
申请人： Takahisa Sato , Akihito Yamano , Shoichi Yasukawa , Hiroki Yoshida , Kensaku Hamada
发明人： Takahisa Sato , Akihito Yamano , Shoichi Yasukawa , Hiroki Yoshida , Kensaku Hamada
IPC分类号： G01N23/223 , G21K4/00
CPC分类号： G01N23/20
摘要： A specific macromolecule crystal evaluating device according to the present invention is equipped with a sample detecting stage for detecting a protein crystal in a sample container, an X-ray measuring stage that is spaced from the sample detecting stage and carries out X-ray diffraction measurement of the protein crystal, a feeding unit for feeding the sample container from the sample detecting stage to the X-ray measuring stage, and a central processing unit for recognizing the position of the protein crystal on the basis of the information achieved in the sample detecting stage and controlling the feeding unit on the basis of the position information to position the protein crystal to a sample disposing portion of the X-ray measuring stage.
摘要翻译：本发明的特定的高分子晶体评价装置具备：样本检测台，用于检测样本容器中的蛋白质晶体，与样本检测级间隔开的X射线衍射测定的蛋白质晶体的供给单元，用于将样本容器从样本检测阶段进给到X射线测量台的进给单元，以及用于基于样本检测中获得的信息来识别蛋白质晶体的位置的中央处理单元并且基于所述位置信息控制所述供给单元，以将所述蛋白质晶体定位到所述X射线测量台的样品设置部。

5. 发明授权

US08876972B2 Crystallization device 有权
标题翻译：结晶装置
公开(公告)号：US08876972B2
公开(公告)日：2014-11-04
申请号：US11879694
申请日：2007-07-17
申请人： Tomokazu Hasegawa , Kensaku Hamada , Masaru Sato , Moritoshi Motohara
发明人： Tomokazu Hasegawa , Kensaku Hamada , Masaru Sato , Moritoshi Motohara
IPC分类号： C30B29/58 , B01D9/00
CPC分类号： B01D9/005
摘要： A crystallization device is for protein crystallization with a small amount of a sample in the liquid to liquid diffusion method. It is easy to fill the device with protein solution and precipitant solution and easy to pick up grown crystals from the device. The device comprises a channel plate made of polydimethylsiloxane (PDMS) and the first and second cover sheets made of polyethylene terephthalate. The channel plate includes at least one elongated channel having one side which extends in the longitudinal direction of the channel, the one side being exposed at the bottom surface of the channel plate. The channel has both ends which communicate with a protein solution inlet and a precipitant solution inlet respectively. The channel also communicates midway with a gel inlet and a vent hole. When picking up grown crystals from the device, the second cover sheet is cut off with a cutter knife so that the channel is exposed.
摘要翻译：结晶装置用于在液体/液体扩散方法中用少量样品进行蛋白质结晶。使用蛋白质溶液和沉淀剂溶液容易填充设备，并容易从设备中取出生长的晶体。该装置包括由聚二甲基硅氧烷（PDMS）制成的通道板和由聚对苯二甲酸乙二醇酯制成的第一和第二覆盖片。通道板包括至少一个细长通道，其具有在通道的纵向方向上延伸的一侧，一侧在通道板的底表面处露出。通道的两端分别与蛋白质溶液入口和沉淀剂溶液入口分别连通。该通道还与凝胶入口和通气孔中途连通。当从设备拾起生长的晶体时，用切割刀切割第二覆盖片，使得通道暴露。

6. 发明申请

US20060266954A1 Specific macromolecule crystal evaluator 失效
标题翻译：特定的大分子晶体评估器
公开(公告)号：US20060266954A1
公开(公告)日：2006-11-30
申请号：US10568740
申请日：2004-08-18
申请人： Takahisa Sato , Akihito Yamano , Shoichi Yasukawa , Hiroki Yoshida , Kensaku Hamada
发明人： Takahisa Sato , Akihito Yamano , Shoichi Yasukawa , Hiroki Yoshida , Kensaku Hamada
IPC分类号： G21G5/00 , A61N5/00
CPC分类号： G01N23/20
摘要： A specific macromolecule crystal evaluating device according to the present invention is equipped with a sample detecting stage for detecting a protein crystal in a sample container, an X-ray measuring stage that is spaced from the sample detecting stage and carries out X-ray diffraction measurement of the protein crystal, a feeding unit for feeding the sample container from the sample detecting stage to the X-ray measuring stage, and a central processing unit for recognizing the position of the protein crystal on the basis of the information achieved in the sample detecting stage and controlling the feeding unit on the basis of the position information to position the protein crystal to a sample disposing portion of the X-ray measuring stage.
摘要翻译：本发明的特定的高分子晶体评价装置具备：样本检测台，用于检测样本容器中的蛋白质晶体，与样本检测级间隔开的X射线衍射测定的蛋白质晶体的供给单元，用于将样本容器从样本检测阶段进给到X射线测量台的进给单元，以及用于基于样本检测中获得的信息来识别蛋白质晶体的位置的中央处理单元并且基于所述位置信息控制所述供给单元，以将所述蛋白质晶体定位到所述X射线测量台的样品设置部。

7. 发明申请

US20110164795A1 METHOD OF DETECTING SPECIFIC POLYMER CRYSTAL 有权
标题翻译：检测特定聚合物晶体的方法
公开(公告)号：US20110164795A1
公开(公告)日：2011-07-07
申请号：US13006883
申请日：2011-01-14
申请人： Akihito YAMANO , Takahisa Sato , Hiroki Yoshida , Motohide Yoshimura , Kensaku Hamada
发明人： Akihito YAMANO , Takahisa Sato , Hiroki Yoshida , Motohide Yoshimura , Kensaku Hamada
IPC分类号： G06K9/00
CPC分类号： G01N21/6486 , G01N21/6452 , G01N21/6458
摘要： In a specific macromolecule crystal detecting method according to the present invention, ultraviolet light is irradiated to sample solution, and a fluorescent image emitted from a sample in the sample solution is detected to detect specific macromolecules in the sample solution. Furthermore, by detecting the outline of the sample from the visible light image of the sample contained in the sample solution, the crystal is discriminated from other materials on the basis of the outline. By integrating the detection results of the fluorescent image and the visible light image, the specific macromolecule crystal is detected from the sample solution.
摘要翻译：在根据本发明的特定的大分子晶体检测方法中，对样品溶液照射紫外光，并且检测从样品溶液中的样品发射的荧光图像，以检测样品溶液中的特定大分子。此外，通过从包含在样品溶液中的样品的可见光图像中检测样品的轮廓，基于轮廓来鉴别晶体与其它材料。通过整合荧光图像和可见光图像的检测结果，从样品溶液中检测特定的大分子晶体。

8. 发明申请

US20080019888A1 Crystallization device 有权
标题翻译：结晶装置
公开(公告)号：US20080019888A1
公开(公告)日：2008-01-24
申请号：US11879694
申请日：2007-07-17
申请人： Tomokazu Hasegawa , Kensaku Hamada , Masaru Sato , Moritoshi Motohara
发明人： Tomokazu Hasegawa , Kensaku Hamada , Masaru Sato , Moritoshi Motohara
IPC分类号： B01D9/02
CPC分类号： B01D9/005
摘要： A crystallization device is for protein crystallization with a small amount of a sample in the liquid to liquid diffusion method. It is easy to fill the device with protein solution and precipitant solution and easy to pick up grown crystals from the device. The device comprises a channel plate made of polydimethylsiloxane (PDMS) and the first and second cover sheets made of polyethylene terephthalate. The channel plate includes at least one elongated channel having one side which extends in the longitudinal direction of the channel, the one side being exposed at the bottom surface of the channel plate. The channel has both ends which communicate with a protein solution inlet and a precipitant solution inlet respectively. The channel also communicates midway with a gel inlet and a vent hole. When picking up grown crystals from the device, the second cover sheet is cut off with a cutter knife so that the channel is exposed.
摘要翻译：结晶装置用于在液体/液体扩散方法中用少量样品进行蛋白质结晶。使用蛋白质溶液和沉淀剂溶液容易填充设备，并容易从设备中取出生长的晶体。该装置包括由聚二甲基硅氧烷（PDMS）制成的通道板和由聚对苯二甲酸乙二醇酯制成的第一和第二覆盖片。通道板包括至少一个细长通道，其具有在通道的纵向方向上延伸的一侧，一侧在通道板的底表面处露出。通道的两端分别与蛋白质溶液入口和沉淀剂溶液入口分别连通。该通道还与凝胶入口和通气孔中途连通。当从设备拾起生长的晶体时，用切割刀切割第二覆盖片，使得通道暴露。

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