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1. 发明申请

US20060252923A1 Nucleotide compositions comprising photocleavable markers and methods of preparation thereof 有权
公开(公告)号：US20060252923A1
公开(公告)日：2006-11-09
申请号：US11351996
申请日：2006-02-09
申请人： Jerzy Olejnik , Edyta Krzymanska-Olejnik , Kenneth Rothschild
发明人： Jerzy Olejnik , Edyta Krzymanska-Olejnik , Kenneth Rothschild
IPC分类号： C12P19/34 , C07H21/04 , C07H21/02
CPC分类号： A61K49/0032 , A61K49/0021 , A61K49/0052 , C07H19/00 , C07H19/04 , C07H21/02 , C07H21/04
摘要： Labelled nucleotides and polynucleotides useful in the sequencing of nucleic acids are described. Methods of preparing photocleavable marker nucleotides and photocleavable marker-polynucleotide conjugates are described. Such photocleavable markere nucleotides can be incorporated into nucleic acid so as to create photocleavable marker-polynucleotide conjugates.

2. 发明申请

US20060024704A1 Detection of markers in nascent proteins 失效
公开(公告)号：US20060024704A1
公开(公告)日：2006-02-02
申请号：US11145781
申请日：2005-06-06
申请人： Kenneth Rothschild , Jerzy Olejnik , Sanjay Sonar
发明人： Kenneth Rothschild , Jerzy Olejnik , Sanjay Sonar
IPC分类号： C12Q1/68 , G01N33/53 , G01N1/28
CPC分类号： C07D207/404 , A61K38/00 , A61K41/0042 , C07C205/45 , C07C205/57 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/68 , G01N33/532 , G01N33/582
摘要： The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system. The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other mammals, and kits which may be used for the detection of diseases and disorders.

3. 发明申请

US20050032078A1 Methods for the detection, analysis and isolation of nascent proteins 审中-公开
公开(公告)号：US20050032078A1
公开(公告)日：2005-02-10
申请号：US10719523
申请日：2003-11-21
申请人： Kenneth Rothschild , Sadanand Gite , Jerzy Olejnik
发明人： Kenneth Rothschild , Sadanand Gite , Jerzy Olejnik
IPC分类号： G01N33/53 , C07K1/22 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/10 , C12N15/67 , C12P21/02 , G01N33/566 , G01N33/68 , C12Q1/68 , C07H21/04
CPC分类号： C12P21/00 , C12N15/10 , C12N15/67 , C12P21/02 , G01N33/6803
摘要： This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) dyes.

4. 发明申请

US20070020643A1 Photocleavable bioreactive agents 失效
标题翻译：可透光的生物反应剂
公开(公告)号：US20070020643A1
公开(公告)日：2007-01-25
申请号：US11326021
申请日：2006-01-05
申请人： Kenneth Rothschild , Sanjay Sonar , Jerzy Olejnik
发明人： Kenneth Rothschild , Sanjay Sonar , Jerzy Olejnik
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6876 , A61K38/00 , A61K41/0042 , C07C205/45 , C07C205/57 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/6823 , G01N33/5306 , G01N33/532 , G01N33/58 , C12Q2523/319
摘要： This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.
摘要翻译：本发明涉及可用于从复杂混合物检测和分离靶组分的试剂和缀合物，例如来自生物样品的核酸，来自体液的细胞和来自翻译反应的新生蛋白。试剂包含与光反应性部分结合的可检测部分。缀合物包括通过共价界限偶联到底物的试剂，其可以通过施用电磁辐射被选择性地切割。目标可以用可检测分子标记的物质可以很容易地识别，并与物质的异源混合物分离。缀合物暴露于放射线以功能形式释放靶，完全不变。使用可光致发光的分子前体作为共轭物，标记可以并入大分子中，分离出新生大分子，并将标记完全除去。本发明还涉及用这些缀合物分离的靶，其可用作可以施用于人和其它哺乳动物的药剂或组合物。有用的组合物包括生物制剂如核酸，蛋白质，脂质和细胞因子。缀合物也可用于监测药物组合物在体内和诊断，治疗和预防目的中的途径和半衰期。本发明还涉及可用于检测疾病，病症和物质复杂背景中的几乎任何单一物质的试剂和缀合物的试剂盒。

5. 发明申请

US20060275750A1 Bioreactive agents 失效
公开(公告)号：US20060275750A1
公开(公告)日：2006-12-07
申请号：US11364476
申请日：2006-02-28
申请人： Kenneth Rothschild , Sanjay Sonar , Jerzy Olejnik
发明人： Kenneth Rothschild , Sanjay Sonar , Jerzy Olejnik
IPC分类号： C12Q1/70 , C12Q1/68 , G01N33/53 , C07D498/02 , C07K14/47
CPC分类号： C12Q1/6876 , A61K38/00 , A61K41/0042 , C07C205/45 , C07C205/57 , C07D207/404 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/6823 , G01N33/5306 , G01N33/532 , G01N33/58 , C12Q2523/319
摘要： This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as nucleic acids from biological samples, cells from bodily fluids, and nascent proteins from translation reactions. Agents comprise a detectable moiety bound to a photoreactive moiety. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals. Useful compositions include biological agents such as nucleic acids, proteins, lipids and cytokines. Conjugates can also be used to monitor the pathway and half-life of pharmaceutical composition in vivo and for diagnostic, therapeutic and prophylactic purposes. The invention also relates to kits comprised of agents and conjugates that can be used for the detection of diseases, disorders and nearly any individual substance in a complex background of substances.

6. 发明申请

US20070172849A1 Detection of markers in nascent proteins 审中-公开
标题翻译：新生蛋白中标记物的检测
公开(公告)号：US20070172849A1
公开(公告)日：2007-07-26
申请号：US11589425
申请日：2006-10-30
申请人： Kenneth Rothschild , Jerzy Olejnik , Sanjay Sonar
发明人： Kenneth Rothschild , Jerzy Olejnik , Sanjay Sonar
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C07D207/404 , A61K38/00 , A61K41/0042 , C07C205/45 , C07C205/57 , C07D311/16 , C07D405/12 , C07D495/04 , C07H19/04 , C07H21/00 , C07K1/1077 , C07K1/13 , C12P21/00 , C12Q1/68 , G01N33/532 , G01N33/582
摘要： The invention is directed to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives, or substances recognized by the protein synthesizing machinery. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent,protein from other components of the translation system. The invention also comprises proteins prepared using misaminoacylated tRNAs which can be utilized in pharmaceutical compositions for the treatment of diseases and disorders in humans and other mammals, and kits which may be used for the detection of diseases and disorders.
摘要翻译：本发明涉及用于在细胞或无细胞翻译系统中翻译的新生蛋白的非放射性标记，检测，定量和分离的方法。 tRNA分子被非放射性标记物错氨基酰化，其可以是非天然氨基酸，氨基酸类似物或衍生物，或由蛋白质合成机理识别的物质。标记可以包含可切割部分，可检测标记，报告物质，其中掺入蛋白质的标记可以与非组合标记物或促进从翻译系统的其他组分检测和分离新生蛋白质的偶联剂区分开来。本发明还包括使用可用于治疗人类和其他哺乳动物疾病和病症的药物组合物中的使用错氨基酰化tRNA制备的蛋白质，以及可用于检测疾病和病症的试剂盒。

7. 发明申请

US20070072214A1 Detection of truncation mutations by mass spectrometry 失效
标题翻译：通过质谱检测截短突变
公开(公告)号：US20070072214A1
公开(公告)日：2007-03-29
申请号：US11487128
申请日：2006-07-14
申请人： Alex Garvin , Sadanand Gite , Vladislav Bergo , Kenneth Rothschild
发明人： Alex Garvin , Sadanand Gite , Vladislav Bergo , Kenneth Rothschild
IPC分类号： C12Q1/68 , C12N15/00
CPC分类号： G01N33/6848
摘要： This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as a mass difference.
摘要翻译：本发明涉及通过新生蛋白的质谱检测和分析，特别是在细胞或无细胞翻译系统内翻译的截短蛋白质。引入这些新生蛋白质的N-末端和C-末端表位允许快速和有效的分离以及质量差异。

8. 发明申请

US20060068449A1 N-terminal and C-terminal markers in nascent proteins 失效
公开(公告)号：US20060068449A1
公开(公告)日：2006-03-30
申请号：US10993568
申请日：2004-11-19
申请人： Kenneth Rothschild , Sadanand Gite , Jerzy Olejnik
发明人： Kenneth Rothschild , Sadanand Gite , Jerzy Olejnik
IPC分类号： G01N33/53 , C07K16/46
CPC分类号： G01N33/582 , C07K16/40 , C12P21/02 , G01N33/6803
摘要： This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Methods are described for incorporating N-terminal, C-terminal and (optionally) affinity markers into a nascent protein

9. 发明申请

US20060024781A1 Detection of truncation mutations by mass spectrometry 有权
公开(公告)号：US20060024781A1
公开(公告)日：2006-02-02
申请号：US10903612
申请日：2004-07-30
申请人： Alex Garvin , Sadanand Gite , Vladislav Bergo , Kenneth Rothschild
发明人： Alex Garvin , Sadanand Gite , Vladislav Bergo , Kenneth Rothschild
IPC分类号： G01N33/53 , C12P21/06 , G01N33/00
CPC分类号： G01N33/6848
摘要： This invention relates to the detection and analysis by mass spec of nascent proteins, and in particular truncated proteins, translated within cellular or cell-free translation systems. N-terminal and C-terminal epitopes introduced into these nascent proteins permit rapid and efficient isolation, as well as a mass difference.

10. 发明申请

US20050085630A1 Photocleavable isotope-coded affinity tags 有权
标题翻译：可透光同位素编码的亲和标签
公开(公告)号：US20050085630A1
公开(公告)日：2005-04-21
申请号：US10687297
申请日：2003-10-16
申请人： Jerzy Olejnik , Kenneth Rothschild
发明人： Jerzy Olejnik , Kenneth Rothschild
IPC分类号： A61K20060101 , C07C233/56 , C07D495/04 , C07K14/00 , G01N33/532
CPC分类号： G01N33/532 , C07D495/04 , C07K1/1077 , C07K1/13 , C07K1/14 , C07K1/16 , C07K1/22 , G01N2458/15 , Y10T436/24
摘要： This invention relates to agents and conjugates that can be used to detect and isolate target components from complex mixtures such as proteins and protein fragments from biological samples from in vivo and in vitro sources. Agents comprise a detectable group bound to a photoreactive group. Conjugates comprise agents coupled to substrates by covalent bounds which can be selectively cleaved with the administration of electromagnetic radiation. Targets substances labeled with detectable molecules can be easily identified and separated from a heterologous mixture of substances. Exposure of the conjugate to radiation releases the target in a functional form and completely unaltered. Using photocleavable molecular precursors as the conjugates, label can be incorporated into macromolecules, the nascent macromolecules isolated and the label completely removed. The invention also relates to targets isolated with these conjugates which may be useful as pharmaceutical agents or compositions that can be administered to humans and other mammals.
摘要翻译：本发明涉及可用于从体内和体外来源的生物样品的复杂混合物如蛋白质和蛋白质片段检测和分离靶组分的试剂和缀合物。试剂包含与光反应性基团结合的可检测基团。缀合物包括通过共价界限偶联到底物的试剂，其可以通过施用电磁辐射被选择性地切割。目标可以用可检测分子标记的物质可以很容易地识别，并与物质的异源混合物分离。缀合物暴露于放射线以功能形式释放靶，完全不变。使用可光致发光的分子前体作为共轭物，标记可以并入大分子中，分离出新生大分子，并将标记完全除去。本发明还涉及用这些缀合物分离的靶，其可用作可以施用于人和其它哺乳动物的药剂或组合物。

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