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1. 发明授权

US08586890B2 Methods of separating, identifying and dispensing specimen and device therefor, and analyzing device method 有权
标题翻译：分离，识别和分配样品及其装置的方法，以及分析装置方法
公开(公告)号：US08586890B2
公开(公告)日：2013-11-19
申请号：US13561941
申请日：2012-07-30
申请人： Toru Takahashi , Ken Tsukii , Jie Xu
发明人： Toru Takahashi , Ken Tsukii , Jie Xu
IPC分类号： B07C5/00
CPC分类号： G01N15/1459 , B01F5/0057 , B01F11/0074 , B07C5/00 , G01N1/286 , G01N15/1427 , G01N15/1436 , G01N2015/1075 , G01N2035/1062
摘要： A specimen sorter includes a separating device to remove a specimen from a container, a detection device to identify the specimen as a target specimen or a non-target specimen, and a dispensing device to dispense the specimen. The dispensing device includes a dispensing nozzle to contact a vibration member to form liquid droplets at a front end of the dispensing nozzle and to dispense the liquid droplets, a first container to collect the liquid droplets dispensed from the dispensing nozzle that contain the target specimen, and a second container to collect the liquid droplets dispensed from the dispensing nozzle that contain the non-target specimen.
摘要翻译：样品分选机包括从容器中取出试样的分离装置，将样品识别为目标试样或非目标试样的检测装置以及分配试样的分注装置。分配装置包括分配喷嘴，其接触振动构件以在分配喷嘴的前端处形成液滴并分配液滴;第一容器，用于收集从包含目标样本的分配喷嘴分配的液滴; 以及用于收集从分配喷嘴分配的含有非目标样品的液滴的第二容器。

2. 发明授权

US08211708B2 Optical measuring device and method therefor 有权
标题翻译：光学测量装置及其方法
公开(公告)号：US08211708B2
公开(公告)日：2012-07-03
申请号：US12610396
申请日：2009-11-02
申请人： Ken Tsukii , Toru Takahashi , Shinichi Taguchi , Jie Xu
发明人： Ken Tsukii , Toru Takahashi , Shinichi Taguchi , Jie Xu
IPC分类号： G01N21/59 , G01N21/00 , C12Q1/02 , C12Q1/00
CPC分类号： G01N21/645 , G01N15/147 , G01N21/532 , G01N2015/1497 , G01N2021/4709 , G01N2021/4726 , G01N2021/4742
摘要： The light measurement apparatus of the invention, in which the light is irradiated to the sample dispersed in the liquid flowing through the flow passage, is used for measuring optical information of the sample. The apparatus includes a light source portion 20 for irradiating the irradiating light L to the liquid 11, a light receiving portion 31 to receive the optical information of the sample S including the irradiating light transmitted through the liquid to generate a receiving light signal SG1, under a condition in which the liquid is irradiated by the irradiating light of the light source portion 20 in a state that a relative position of the sample S to the irradiating light varies at constant speed, a measurement portion 120 for measuring variation of the receiving light signal according to the sample.
摘要翻译：本发明的光测量装置用于测量样品的光学信息，其中光照射到分散在流过流道的液体中的样品。该装置包括用于将照射光L照射到液体11的光源部分20，用于接收包含透过液体的照射光的样品S的光学信息以产生接收光信号SG1的光接收部分31，在样品S与照射光的相对位置以恒定速度变化的状态下，由光源部20的照射光照射液体的状态，测定接收光信号的变化的测定部120 根据样品。

3. 发明申请

US20110177544A1 METHOD FOR DISTINGUISHING AND SORTING OF CELLS AND DEVICE THEREFOR 有权
标题翻译：用于细胞分离和分离的方法及其装置
公开(公告)号：US20110177544A1
公开(公告)日：2011-07-21
申请号：US13001128
申请日：2009-06-19
申请人： Toru Takahashi , Ken Tsukii , Jie Xu
发明人： Toru Takahashi , Ken Tsukii , Jie Xu
IPC分类号： C12Q1/02 , C12M1/34
CPC分类号： G01N15/147 , G01N2015/1438 , G01N2015/1493 , G01N2015/1497
摘要： A method for distinguishing and sorting cells characterized by comprising distinguishing and sorting a specific cell mass or a part of the cells in the cell mass with the use of transmitted light data reflecting the morphological characteristics of the cells such as size and shape optionally together with side-scattering light data reflecting the characteristics of the internal structure of the cells. The part of the cells in the specific cell mass as described above are at the G1 stage or at a part of the M stage in the cell cycle. A part of the cells at the G1 stage are referred to as the left bottom line in an analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data, while a part of the cells at the M stage are referred to as the right bottom line in the analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data.
摘要翻译：一种用于区分和分选细胞的方法，其特征在于包括使用反映细胞形态学特征的透射光数据（例如尺寸和形状）来选择性地与侧面区分和分选细胞块中的特定细胞块或细胞的一部分反映细胞内部结构特征的散射光数据。如上所述的特定细胞团中的细胞的部分在细胞周期中处于G1阶段或M阶段的一部分。在G1阶段的细胞的一部分在细胞的分析色散图中被称为左下底线，其中横坐标表示透射光数据，而M阶段的一部分细胞被称为右细胞的分析色散图中的底线，其中横坐标表示透射的光数据。

4. 发明申请

US20110177489A1 SPECIMEN IDENTIFICATION AND DISPENSATION DEVICE AND SPECIMEN IDENTIFICATION AND DISPENSATION METHOD 有权
标题翻译：样本识别和分配设备和样本识别和分配方法
公开(公告)号：US20110177489A1
公开(公告)日：2011-07-21
申请号：US13003403
申请日：2008-07-09
申请人： Toru Takahashi , Jie Xu , Ken Tsukii
发明人： Toru Takahashi , Jie Xu , Ken Tsukii
IPC分类号： C12Q3/00 , C12M1/36
CPC分类号： B01L3/0262 , B01L2200/0647 , B01L2300/0654 , B01L2300/0838 , G01N35/1016 , G01N35/1081 , G01N2035/1034
摘要： A specimen identification and dispensation device includes an optical measurement device which is an identification part for measuring and identifying optical information on a specimen by emitting exciting light to the specimen being a measurement target dispersed in a liquid flowing through inside of a capillary, a dispensation part for dispensing the identified specimen into wells being sections to be dispensed through a nozzle, and a concentration adjustment part which adjusts the number of the specimens contained in an aliquot solution to a desired number according to the concentration of a sample liquid and the amount of the aliquot solution. The dispensation part is movable three-dimensionally with respect to the identification part and the nozzle.
摘要翻译：样本识别和分配装置包括：光学测量装置，其是用于通过向作为分散在流过毛细管内部的液体中的测量对象的样本发射激发光来测量和识别样本上的光学信息的识别部分，分配部分用于将识别的样品分配到通过喷嘴分配的部分的孔中;以及浓度调节部，其根据样品液体的浓度将等分试样溶液中包含的试样的数量调整到所需数量，等分溶液。分配部分相对于识别部分和喷嘴三维移动。

5. 发明申请

US20100233753A1 OPTICAL MEASURING DEVICE AND METHOD THEREFOR 有权
标题翻译：光学测量装置及其方法
公开(公告)号：US20100233753A1
公开(公告)日：2010-09-16
申请号：US12610396
申请日：2009-11-02
申请人： Ken TSUKII , Toru TAKAHASHI , Shinichi TAGUCHI , Jie XU
发明人： Ken TSUKII , Toru TAKAHASHI , Shinichi TAGUCHI , Jie XU
IPC分类号： C12Q1/02 , G01N21/59
CPC分类号： G01N21/645 , G01N15/147 , G01N21/532 , G01N2015/1497 , G01N2021/4709 , G01N2021/4726 , G01N2021/4742
摘要： The light measurement apparatus of the invention, in which the light is irradiated to the sample dispersed in the liquid flowing through the flow passage, is used for measuring optical information of the sample. The apparatus includes a light source portion 20 for irradiating the irradiating light L to the liquid 11, a light receiving portion 31 to receive the optical information of the sample S including the irradiating light transmitted through the liquid to generate a receiving light signal SG1, under a condition in which the liquid is irradiated by the irradiating light of the light source portion 20 in a state that a relative position of the sample S to the irradiating light varies at constant speed, a measurement portion 120 for measuring variation of the receiving light signal according to the sample.
摘要翻译：本发明的光测量装置用于测量样品的光学信息，其中光照射到分散在流过流道的液体中的样品。该装置包括用于将照射光L照射到液体11的光源部分20，用于接收包含透过液体的照射光的样品S的光学信息以产生接收光信号SG1的光接收部分31，在样品S与照射光的相对位置以恒定速度变化的状态下，由光源部20的照射光照射液体的状态，测定接收光信号的变化的测定部120 根据样品。

6. 发明申请

US20090021722A1 Specimen optical information recognizing device and its recognizing method 有权
标题翻译：标本光信息识别装置及其识别方法
公开(公告)号：US20090021722A1
公开(公告)日：2009-01-22
申请号：US11659132
申请日：2005-08-01
申请人： Ken Tsukii , Jie Xu , Kenichi Kimura , Satoshi Sugiyama
发明人： Ken Tsukii , Jie Xu , Kenichi Kimura , Satoshi Sugiyama
IPC分类号： G01N21/00 , G01M7/00 , G01F23/00 , A61N5/00
CPC分类号： G01N21/6452 , G01N21/253 , G01N2201/08
摘要： A specimen optical information recognizing device comprises a specimen containing section (14) containing a specimen (12) to be measured, a specimen measuring section (20) having a light source (16) for outputting light for observing the specimen (12) and a photodetecting section (18) for collecting optical information from the specimen (12), and an optical waveguide (22) for propagating light between the specimen containing section (14) and the specimen measuring section (20). The optical information on the specimen is recognized by using the measurement values acquired under at least two measurement conditions. The specimen optical information recognizing device may further comprises a measurement auxiliary liquid interposed between the end of the optical waveguide and the specimen. Further, the specimen containing section may have a feature that the longitudinal cross section is of a recessed shape, and the aperture depth of the recessed portion is greater than the aperture diameter. The specimen containing section may further have a feature that a through hole is provided at least in a part near the bottom of the specimen containing section.
摘要翻译：样本光学信息识别装置包括：样本容纳部（14），其包含待测试样本（12）;样本测量部（20），具有用于输出用于观察样本（12）的光的光源（16）;以及用于从样本（12）收集光学信息的光电检测部分（18）和用于在样本容纳部分（14）和样本测量部分（20）之间传播光的光波导（22）。通过使用在至少两个测量条件下获取的测量值来识别样品上的光学信息。样本光学信息识别装置还可以包括插入在光波导的端部和样本之间的测量辅助液体。此外，试样容纳部可以具有纵向截面为凹状的特征，凹部的开口深度大于孔径。样品容纳部还可以具有至少在样本容纳部的底部附近的部分设置通孔的特征。

7. 发明申请

US20080297793A1 SMALL OBJECT IDENTIFYING DEVICE AND ITS IDENTIFYING METHOD 有权
公开(公告)号：US20080297793A1
公开(公告)日：2008-12-04
申请号：US12189001
申请日：2008-08-08
申请人： Masahisa Noguchi , Ken Tsukii , Hideji Tajima
发明人： Masahisa Noguchi , Ken Tsukii , Hideji Tajima
IPC分类号： G01J3/30
CPC分类号： G01N15/1456 , G01N2015/1488 , G01N2015/1497
摘要： A small object identifying device and its identifying method according to which a large number of small objects can be identified. In one embodiment, the device includes a dispersion region section which disperses a large quantity of several kinds of small objects which are labeled by a combination of the presence/absence or measure of label elements of several kinds. A measuring device distributes and associates kinds of said label elements to two or more measurement points and measures the presence/absence or the measure of said label elements of the kinds which have been associated with respective measurement points. An identifying section associates the measurement results measured at each measurement point to thereby identify said small objects.

8. 发明申请

US20080257072A1 Methods of Separating, Identifying and Dispensing Specimen and Device Therefor, and Analyzing Device Method 有权
标题翻译：分离，鉴定和分配样品及其装置的方法和分析装置方法
公开(公告)号：US20080257072A1
公开(公告)日：2008-10-23
申请号：US11587210
申请日：2005-04-25
申请人： Toru Takahashi , Ken Tsukii , Jie Xu
发明人： Toru Takahashi , Ken Tsukii , Jie Xu
IPC分类号： B01L3/02 , A61L2/20 , G01N23/00 , G01P3/36
CPC分类号： G01N15/1459 , B01F5/0057 , B01F11/0074 , B07C5/00 , G01N1/286 , G01N15/1427 , G01N15/1436 , G01N2015/1075 , G01N2035/1062
摘要： [PROBLEMS] A (a) (b) separation unit can confirms a separation state, a detection unit enhances a lighting efficiency and a light reception sensitivity, and a dispensing unit ensures the normal state of a specimen. [MEANS FOR SOLVING PROBLEMS] A specimen separation device characterized by comprising a container for storing a specimen, a nozzle for sucking and ejecting the specimen from the container, a nozzle operating means for moving the nozzle vertically and laterally, and a nozzle controlling means for controlling the suction force and ejection force of the nozzle. A specimen identification device comprising a nozzle for sucking a specimen from a container storing the specimen, a flow path for allowing the specimen to flow therethrough, and a measuring unit having a monitoring light lighting unit and a light receiving unit for observing (monitoring) the specimen that are installed in a flow path, characterized in that the above nozzle, the flow path and the measuring unit are formed in an integrated structure.
摘要翻译：（问题）A（a）（b）分离单元可以确认分离状态，检测单元提高照明效率和光接收灵敏度，并且分配单元确保样本的正常状态。用于解决问题的手段一种试样分离装置，其特征在于，包括容纳容器的容器，用于从容器吸取和喷射试样的喷嘴，用于使喷嘴垂直和侧向移动的喷嘴操作装置，以及用于控制喷嘴的吸力和喷射力。一种样本识别装置，包括：用于从容纳所述样本的容器吸取试样的喷嘴，用于使所述试样流过的流路;以及具有监视用光照射单元的测量单元和观察（监视）试样，其安装在流路中，其特征在于，上述喷嘴，流路和测量单元形成为一体的结构。

9. 发明授权

US07426027B2 Small object identifying device and its identifying method 失效
标题翻译：小物体识别装置及其识别方法
公开(公告)号：US07426027B2
公开(公告)日：2008-09-16
申请号：US11867585
申请日：2007-10-04
申请人： Masahisa Noguchi , Ken Tsukii , Hideji Tajima
发明人： Masahisa Noguchi , Ken Tsukii , Hideji Tajima
IPC分类号： G01N21/64
CPC分类号： G01N15/1456 , G01N2015/1488 , G01N2015/1497
摘要： A small object identifying device and its identifying method according to which a large number of small objects can be identified. In one embodiment, the device includes a dispersion region section which disperses a large quantity of several kinds of small objects which are labeled by a combination of the presence/absence or measure of label elements of several kinds. A measuring device distributes and associates kinds of said label elements to two or more measurement points and measures the presence/absence or the measure of said label elements of the kinds which have been associated with respective measurement points. An identifying section associates the measurement results measured at each measurement point to thereby identify said small objects.
摘要翻译：小物体识别装置及其识别方法，根据该方法可以识别大量的小物体。在一个实施例中，该装置包括分散大量几种小物体的分散区域区域，这些小物体由几种标签元素的存在/不存在或测量的组合标记。测量装置将所述标签元件的种类分配并关联到两个或更多个测量点，并且测量已经与各个测量点相关联的种类的所述标签元素的存在/不存在或测量。识别部分将在每个测量点处测量的测量结果相关联，从而识别所述小物体。

10. 发明申请

US20080037831A1 SMALL OBJECT IDENTIFYING DEVICE AND ITS IDENTIFYING METHOD 失效
标题翻译：小目标识别装置及其识别方法
公开(公告)号：US20080037831A1
公开(公告)日：2008-02-14
申请号：US11867585
申请日：2007-10-04
申请人： Masahisa Noguchi , Ken Tsukii , Hideji Tajima
发明人： Masahisa Noguchi , Ken Tsukii , Hideji Tajima
IPC分类号： G06K9/00
CPC分类号： G01N15/1456 , G01N2015/1488 , G01N2015/1497
摘要： A small object identifying device and its identifying method according to which a large number of small objects can be identified. In one embodiment, the device includes a dispersion region section which disperses a large quantity of several kinds of small objects which are labeled by a combination of the presence/absence or measure of label elements of several kinds. A measuring device distributes and associates kinds of said label elements to two or more measurement points and measures the presence/absence or the measure of said label elements of the kinds which have been associated with respective measurement points. An identifying section associates the measurement results measured at each measurement point to thereby identify said small objects.
摘要翻译：小物体识别装置及其识别方法，根据该方法可以识别大量的小物体。在一个实施例中，该装置包括分散大量几种小物体的分散区域区域，这些小物体由几种标签元素的存在/不存在或测量的组合标记。测量装置将所述标签元件的种类分配并关联到两个或更多个测量点，并且测量已经与各个测量点相关联的种类的所述标签元素的存在/不存在或测量。识别部分将在每个测量点处测量的测量结果相关联，从而识别所述小物体。

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