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1. 发明授权

US07105294B2 Method and probes for the detection of chromosome aberrations 有权
公开(公告)号：US07105294B2
公开(公告)日：2006-09-12
申请号：US10229110
申请日：2002-08-27
申请人： Jacobus Johannus Maria Van Dongen , Karl-Johan Pluzek , Kirsten Vang Nielsen , Kim Adelhorst
发明人： Jacobus Johannus Maria Van Dongen , Karl-Johan Pluzek , Kirsten Vang Nielsen , Kim Adelhorst
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6827 , C12Q1/6841 , C12Q1/6883 , C12Q2600/156 , C12Q2525/107 , C12Q2565/601
摘要： A novel method for detecting chromosome aberrations is disclosed. More specifically, chromosome aberrations are detected by in situ hybridisation using at least two sets of hybridisation probes, at least one set comprising one or more peptide nucleic acid probes capable of hybridising to specific nucleic acid sequences related to a potential aberration in a chromosome, and at least one set comprising two or more peptide nucleic acid probes capable of hybridising to specific nucleic acid sequences related to another potential aberration in a chromosome. In particular, the method may be used for detecting chromosome aberrations in the form of breakpoints.

2. 发明授权

US5888733A In situ hybridization to detect specific nucleic acid sequences in eucaryotic samples 失效
标题翻译：原位杂交以检测真核样品中的特异性核酸序列
公开(公告)号：US5888733A
公开(公告)日：1999-03-30
申请号：US724824
申请日：1996-10-02
申请人： Jens J. Hyldig-Nielsen , Karl-Johan Pluzek , Tom Just
发明人： Jens J. Hyldig-Nielsen , Karl-Johan Pluzek , Tom Just
IPC分类号： C12Q1/68 , C07H21/04 , C07K5/00
CPC分类号： C12Q1/6841
摘要： Methodologies for determining the presence of specific nucleic acid sequences in a sample of eucaryotic origin using in situ hybridization are described. The in situ hybridization is performed using a hybridization solution comprising at least one binding partner capable of hybridizing to the specific nucleic acid sequence to be determined so as to form hybrids and a hybrid destabilizing agent in an amount effective to decrease the melting temperature of hybrids formed between the nucleic acid and the binding partner so as to increase the specific binding and decrease the non-specific binding. The binding partner used is a polymeric strand of polymerized moieties having a non-cyclic backbone, the polymeric strand being capable of hybridizing to the nucleic acid sequence to be determined. The method is particularly suitable for diagnosing different human diseases such as bacterial and viral infections, genetic diseases and neoplastic disorders.
摘要翻译：描述了使用原位杂交确定真核来源样品中特定核酸序列的存在的方法。原位杂交使用包含至少一个结合配偶体的杂交溶液进行，所述结合配偶体能够与需要测定的特异性核酸序列杂交以形成混合物和混合去稳定剂，其量有效降低形成的杂交体的熔解温度在核酸和结合配偶体之间，以增加特异性结合并降低非特异性结合。使用的结合配偶体是具有非环状主链的聚合部分的聚合物链，所述聚合物链能够与待确定的核酸序列杂交。该方法特别适用于诊断不同的人类疾病，如细菌和病毒感染，遗传疾病和肿瘤性疾病。

3. 发明申请

US20080187934A1 Method and probes for the detection of chromosome aberrations 有权
公开(公告)号：US20080187934A1
公开(公告)日：2008-08-07
申请号：US12071268
申请日：2008-02-19
申请人： Jacobus Johannus Maria Van Dongen , Karl-Johan Pluzek , Kirsten Vang Nielsen , Kim Adelhorst
发明人： Jacobus Johannus Maria Van Dongen , Karl-Johan Pluzek , Kirsten Vang Nielsen , Kim Adelhorst
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6841 , C12Q1/6883 , C12Q2600/156 , C12Q2525/107 , C12Q2565/601
摘要： A novel method for detecting chromosome aberrations is disclosed. More specifically, chromosome aberrations are detected by in situ hybridisation using at least two sets of hybridisation probes, at least one set comprising one or more peptide nucleic acid probes capable of hybridising to specific nucleic acid sequences related to a potential aberration in a chromosome, and at least one set comprising two or more peptide nucleic acid probes capable of hybridising to specific nucleic acid sequences related to another potential aberration in a chromosome. In particular, the method may be used for detecting chromosome aberrations in the form of breakpoints.

4. 发明授权

US5612458A Antibody to PNA/nucleic acid complexes 失效
标题翻译： PNA /核酸复合物的抗体
公开(公告)号：US5612458A
公开(公告)日：1997-03-18
申请号：US361643
申请日：1994-12-22
申请人： Jens J. Hyldig-Nielsen , Karl-Johan Pluzek
发明人： Jens J. Hyldig-Nielsen , Karl-Johan Pluzek
IPC分类号： G01N33/53 , C07K16/44 , C12N15/09 , A61K39/00 , A61K51/10
CPC分类号： C07K16/44
摘要： This invention relates to antibodies to complexes formed between PNA (Peptide Nucleic Acid) and nucleic acids, particularly antibodies to PNA/DNA or PNA/RNA complexes. The preferred antibodies are polyclonal, monoclonal and recombinant antibodies that binds to PNA/DNA or PNA/RNA complexes, but not to single-stranded PNA, double-stranded nucleic acid or single-stranded nucleic acid. Peptide Nucleic Acids (PNA) are newly developed, not naturally occurring compounds comprising a polyamide backbone bearing a plurality of ligands such as naturally occuring nucleobases attached to a polyamide backbone through a suitable linker. PNA oligomers with a backbone of N-(2-aminoethyl)glycin units have a surprising high affinity for complementary nucleic acid forming very stable and specific complexes. This property makes PNA oligomers suitable as hybridization probes for detection of nucleic acids. The usability of PNA as hybridization probes is greatly increased by the present antibodies. The antibodies according to the invention are useful in the capture, recognition, detection, identification or quantitation of nucleic acids in biological samples, via their ability to react with PNA-nucleic acid complexes.
摘要翻译：本发明涉及在PNA（肽核酸）和核酸之间形成的复合物的抗体，特别是针对PNA / DNA或PNA / RNA复合物的抗体。优选的抗体是结合PNA / DNA或PNA / RNA复合物但不与单链PNA，双链核酸或单链核酸结合的多克隆，单克隆和重组抗体。肽核酸（PNA）是新开发的，而不是天然存在的化合物，其包含具有多个配体的聚酰胺主链，例如通过合适的接头天然存在连接到聚酰胺主链的核碱基。具有N-（2-氨基乙基）甘氨酸单元骨架的PNA寡聚体对形成非常稳定和特异性复合物的互补核酸具有惊人的高亲和力。该性质使得PNA低聚物适合作为检测核酸的杂交探针。通过本发明的抗体，PNA作为杂交探针的可用性大大提高。根据本发明的抗体可用于通过其与PNA-核酸复合物反应的能力捕获，识别，鉴定或定量生物样品中的核酸。

5. 发明授权

US07642057B2 Method and probes for the detection of chromosome aberrations 有权
标题翻译：用于检测染色体畸变的方法和探针
公开(公告)号：US07642057B2
公开(公告)日：2010-01-05
申请号：US12071268
申请日：2008-02-19
申请人： Jacobus Johannus Maria Van Dongen , Karl-Johan Pluzek , Kristen Vang Nielsen , Kim Adelhorst
发明人： Jacobus Johannus Maria Van Dongen , Karl-Johan Pluzek , Kristen Vang Nielsen , Kim Adelhorst
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6827 , C12Q1/6841 , C12Q1/6883 , C12Q2600/156 , C12Q2525/107 , C12Q2565/601
摘要： A novel method for detecting chromosome aberrations is disclosed. More specifically, chromosome aberrations are detected by in situ hybridisation using at least two sets of hybridisation probes, at least one set comprising one or more peptide nucleic acid probes capable of hybridising to specific nucleic acid sequences related to a potential aberration in a chromosome, and at least one set comprising two or more peptide nucleic acid probes capable of hybridising to specific nucleic acid sequences related to another potential aberration in a chromosome. In particular, the method may be used for detecting chromosome aberrations in the form of breakpoints.
摘要翻译：公开了一种用于检测染色体畸变的新方法。更具体地，使用至少两组杂交探针通过原位杂交检测染色体畸变，至少一组包含能够与染色体中的潜在像差相关的特定核酸序列杂交的一个或多个肽核酸探针，以及至少一个包含两个或更多个肽核酸探针的组，所述肽核酸探针能够与与染色体中的另一个潜在像差相关的特定核酸序列杂交。特别地，该方法可用于以断点的形式检测染色体畸变。

6. 发明授权

US07368245B2 Method and probes for the detection of chromosome aberrations 有权
标题翻译：用于检测染色体畸变的方法和探针
公开(公告)号：US07368245B2
公开(公告)日：2008-05-06
申请号：US11220718
申请日：2005-09-08
申请人： Jacobus Johannus Maria Van Dongen , Karl-Johan Pluzek , Kirsten Vang Nielsen , Kim Adelhorst
发明人： Jacobus Johannus Maria Van Dongen , Karl-Johan Pluzek , Kirsten Vang Nielsen , Kim Adelhorst
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02
CPC分类号： C12Q1/6827 , C12Q1/6841 , C12Q1/6883 , C12Q2600/156 , C12Q2525/107 , C12Q2565/601
摘要： A novel method for detecting chromosome aberrations is disclosed. More specifically, chromosome aberrations are detected by in situ hybridisation using at least two sets of hybridisation probes, at least one set comprising one or more peptide nucleic acid probes capable of hybridising to specific nucleic acid sequences related to a potential aberration in a chromosome, and at least one set comprising two or more peptide nucleic acid probes capable of hybridising to specific nucleic acid sequences related to another potential aberration in a chromosome. In particular, the method may be used for detecting chromosome aberrations in the form of breakpoints.
摘要翻译：公开了一种用于检测染色体畸变的新方法。更具体地，使用至少两组杂交探针通过原位杂交检测染色体畸变，至少一组包含能够与染色体中的潜在像差相关的特定核酸序列杂交的一个或多个肽核酸探针，以及至少一个包含两个或更多个肽核酸探针的组，所述肽核酸探针能够与与染色体中的另一个潜在像差相关的特定核酸序列杂交。特别地，该方法可用于以断点的形式检测染色体畸变。

7. 发明申请

US20060160106A1 Method and probes for the detection of chromosome aberrations 有权
标题翻译：用于检测染色体畸变的方法和探针
公开(公告)号：US20060160106A1
公开(公告)日：2006-07-20
申请号：US11220718
申请日：2005-09-08
申请人： Jacobus Van Dongen , Karl-Johan Pluzek , Kirsten Nielsen , Kim Adelhorst
发明人： Jacobus Van Dongen , Karl-Johan Pluzek , Kirsten Nielsen , Kim Adelhorst
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6841 , C12Q1/6883 , C12Q2600/156 , C12Q2525/107 , C12Q2565/601
摘要： A novel method for detecting chromosome aberrations is disclosed. More specifically, chromosome aberrations are detected by in situ hybridisation using at least two sets of hybridisation probes, at least one set comprising one or more peptide nucleic acid probes capable of hybridising to specific nucleic acid sequences related to a potential aberration in a chromosome, and at least one set comprising two or more peptide nucleic acid probes capable of hybridising to specific nucleic acid sequences related to another potential aberration in a chromosome. In particular, the method may be used for detecting chromosome aberrations in the form of breakpoints.
摘要翻译：公开了一种用于检测染色体畸变的新方法。更具体地，使用至少两组杂交探针通过原位杂交检测染色体畸变，至少一组包含能够与染色体中的潜在像差相关的特定核酸序列杂交的一个或多个肽核酸探针，以及至少一个包含两个或更多个肽核酸探针的组，所述肽核酸探针能够与与染色体中的另一个潜在像差相关的特定核酸序列杂交。特别地，该方法可用于以断点的形式检测染色体畸变。

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