专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20090258435A1 BIOTIN-RECEPTOR REAGENTS FOR SENSITIVITY MODULATION IN ASSAYS 审中-公开
标题翻译：用于测定灵敏度调节的生物受体试剂
公开(公告)号：US20090258435A1
公开(公告)日：2009-10-15
申请号：US12103245
申请日：2008-04-15
申请人： Sandra A. Lewisch , Pratap Singh , Viral Desai , Karen L. Krakowski , James E. Duffy
发明人： Sandra A. Lewisch , Pratap Singh , Viral Desai , Karen L. Krakowski , James E. Duffy
IPC分类号： G01N33/566 , G01N33/536
CPC分类号： G01N33/532 , G01N33/54393 , G01N33/566
摘要： Methods are disclosed for designing an antibody reagent for use in an assay for the detection of an analyte to obtain an optimum assay sensitivity and/or dynamic range. The antibody reagent is a conjugate of a small molecule attached by a spacer group to an antibody for the analyte. The method comprises controlling, in the preparation of the conjugate, reaction parameters comprising the hydrophobicity or hydrophilicity of the spacer group, the length of the spacer group, the number of molecules of the small molecule attached to the antibody and the point of attachment of the small molecule to the antibody to obtain an optimum assay sensitivity and/or dynamic range. In some embodiments the method comprises preparing two or more conjugates by selecting a set of parameters for each conjugate wherein the set of parameters is different for each conjugate, conducting an assay for the analyte employing each conjugate and selecting for use in the assay the conjugate that provides the optimum assay sensitivity and/or dynamic range.
摘要翻译：公开了设计用于分析物检测以获得最佳测定灵敏度和/或动态范围的测定中的抗体试剂的方法。抗体试剂是通过间隔基团与分析物的抗体连接的小分子的缀合物。该方法包括在共轭物的制备中控制包括间隔基团的疏水性或亲水性，间隔基团的长度，与抗体连接的小分子的分子数目和小分子到抗体以获得最佳测定灵敏度和/或动态范围。在一些实施方案中，该方法包括通过为每个缀合物选择一组参数来制备两种或多种缀合物，其中该组参数对于每个缀合物是不同的，使用每个缀合物进行分析物的测定，并选择用于测定缀合物提供最佳测定灵敏度和/或动态范围。

IPRDB

热门服务

关于我们

友情链接

联系方式