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1. 发明授权

US5180475A System and method for controlling electroosmotic flow 失效
标题翻译：用于控制电渗流的系统和方法
公开(公告)号：US5180475A
公开(公告)日：1993-01-19
申请号：US754797
申请日：1991-09-04
申请人： James E. Young , Douglass McManigill , Jurgen A. Lux
发明人： James E. Young , Douglass McManigill , Jurgen A. Lux
IPC分类号： B01D57/02 , G01N27/447
CPC分类号： G01N27/44752 , G01N27/44713
摘要： A system and method for controlling the rate of electroosmotic flow in capillary electrophoresis. A first high voltage power supply is electrically connected to the inlet end of a capillary tube, while a second high voltage power supply is connected at an outlet end. The potential difference across the capillary tube is therefore the difference between the high voltages. Electroosmotic flow can be controlled by varying the two high voltages correspondingly to maintain the same potential difference but to achieve a different voltage offset relative to ground. Optionally, the voltage offset may be varied during electrophoresis analysis to slow migration during periods in which constituents are closely spaced and to speed migration when constituents are sufficiently spaced apart. A conductive capillary tube coating which is preferably grounded defines an external control area for electroosmotic flow. A detector is located downstream of the external control area, so that the coating is a unitary layer.

2. 发明授权

US5322607A Electrical potential configuration for an electrophoresis system 失效
标题翻译：电泳系统的电位配置
公开(公告)号：US5322607A
公开(公告)日：1994-06-21
申请号：US913123
申请日：1992-07-14
申请人： Richard L. Baer , Jurgen A. Lux , James E. Young
发明人： Richard L. Baer , Jurgen A. Lux , James E. Young
IPC分类号： G01N27/447 , C25B9/00
CPC分类号： G01N27/44752
摘要： An electrophoresis system having separate internal and external voltage gradients that are vectorially coupled to determine zeta potential along the interior surface of a capillary column. The voltage gradient along the exterior of the capillary column is established such that the electrical potential at an on-column detection area is substantially equal to the electrical potential of a detector used to sense electrophoretic migration past the detection area. Typically, the capillary column is grounded at the point of detection, with the exterior voltage gradient being positive-going in one direction from the point of detection and being negative-going in the opposite direction.
摘要翻译：具有分离的内部和外部电压梯度的电泳系统，其被矢量耦合以确定沿着毛细管柱的内表面的ζ电位。建立沿着毛细管柱外部的电压梯度，使得在列检测区域处的电位基本上等于用于感测经过检测区域的电泳迁移的检测器的电位。通常，毛细管柱在检测点接地，外部电压梯度从检测点向一个方向呈正向，并且在相反方向呈负方向。

3. 发明授权

US5240585A Conductive bridge for external control of electroosmotic flow 失效
标题翻译：电渗流外部控制导电桥
公开(公告)号：US5240585A
公开(公告)日：1993-08-31
申请号：US914174
申请日：1992-07-14
申请人： James E. Young , Jurgen A. Lux
发明人： James E. Young , Jurgen A. Lux
IPC分类号： G01N27/447
CPC分类号： G01N27/44752
摘要： An electrophoresis system for controlling electroosmotic flow by affecting a zeta potential, wherein a conductive bridge provides continuity of control. A conductive coating on an optically transparent capillary tube is made of an opaque material which is removed at a detection window for on-column detection of sample constituents past the window. The conductive coating may be allowed to float relative to ground, may be grounded, or may be connected to one or more high voltage power supplies so that a potential gradient is established along the length of the conductive coating. A bridge establishes a low resistance electrical connection across the detection window, thereby establishing a continuity of external control of electroosmotic flow. In a first embodiment, the bridge is a generally flat metallic member that is adhesively bonded to the capillary tube at opposite ends of the detection window. The metallic member includes an aperture along the optical path from the on-column detector to the window. In a second embodiment the bridge is a wire that is bonded to the conductive coating at opposite ends of the window. A third embodiment is to leave a portion of the conductive coating along the length of the window at a region outside of the optical path. Another embodiment is one in which the detection window is at ground potential and the on-column detector itself is used to provide a bridge.
摘要翻译：一种用于通过影响ζ电位来控制电渗流的电泳系统，其中导电桥提供连续的控制。在光学透明的毛细管上的导电涂层由不透明材料制成，其在检测窗口处被去除，用于对通过窗口的样品成分进行柱上检测。可以允许导电涂层相对于地面浮动，可以接地，或者可以连接到一个或多个高压电源，使得沿着导电涂层的长度建立电位梯度。桥梁跨越检测窗口建立了低电阻电连接，从而建立了电渗流的外部控制的连续性。在第一实施例中，桥是大致平坦的金属构件，其在检测窗的相对端处粘合到毛细管上。金属构件包括沿着从柱上检测器到窗口的光路的孔。在第二实施例中，桥是在窗的相对端处结合到导电涂层的导线。第三实施例是在光路外部的区域沿着窗口的长度留下导电涂层的一部分。另一个实施例是其中检测窗处于地电位并且列列检测器本身用于提供桥的实施例。

4. 发明授权

US5114550A Method for the production of gel filled capillaries for capillary gel electrophoresis 失效
标题翻译：生产凝胶填充毛细管毛细管电泳的方法
公开(公告)号：US5114550A
公开(公告)日：1992-05-19
申请号：US694742
申请日：1991-05-02
申请人： Gerhard Schomburg , Jurgen A. Lux
发明人： Gerhard Schomburg , Jurgen A. Lux
IPC分类号： B01D57/02 , G01N27/447
CPC分类号： B01D57/02 , G01N27/44747
摘要： The .gamma.-radiation initiated formation of a polyacrylamide gel for the simplified production of gel filled fused silica capillaries for capiliary gel electrophoresis. The performance and stability of such gel filled capillaries are as good as of those obtained by methods known in the art.

5. 发明授权

US5290587A Method of making an electrophoretic capillary tube 失效
标题翻译：制作电泳毛细管的方法
公开(公告)号：US5290587A
公开(公告)日：1994-03-01
申请号：US914153
申请日：1992-07-14
申请人： James E. Young , Mark Bateman , Jurgen A. Lux
发明人： James E. Young , Mark Bateman , Jurgen A. Lux
IPC分类号： C23C26/02 , C23C4/00 , B05D1/00
CPC分类号： C23C26/02
摘要： A method of making a capillary tube for providing increased control of electroosmotic flow in electrophoretic separation. A resistive coating solution is formed so that a tube coating having a high electrical resistivity may be achieved. An electrically conductive material, such as carbon black, is homogeneously mixed with a polymer, such as polyimide, in a solvent. The content by weight of the electrically conductive material is less than 20 percent and is preferably within 7 percent to 8 percent, relative to the content by weight of the polymer. The resistive coating solution is applied to the exterior of the tube until a thickness is reached which achieves a desired resistance across the coated exterior surface. Typically, a multi-layered coating is formed. The coating solution may be applied while rotating the capillary tube or, alternatively, while the capillary tube is drawn past an applicator.
摘要翻译：一种制造毛细管的方法，用于在电泳分离中提供增加的电渗流控制。形成电阻涂层溶液，使得可以实现具有高电阻率的管涂层。将诸如炭黑的导电材料与聚合物如聚酰亚胺在溶剂中均匀混合。相对于聚合物的重量含量，导电材料的重量含量小于20％，优选在7％-8％以内。将电阻涂层溶液施加到管的外部，直到达到厚度，其在涂覆的外表面上达到期望的电阻。通常，形成多层涂层。可以在旋转毛细管的同时施加涂布溶液，或者当毛细管被拉过涂布器时施加。

6. 发明授权

US5141612A Production of polyacryamide gel filled capillaries for capillary gel electrophoresis 失效
标题翻译：生产用于毛细管凝胶电泳的聚丙烯酰胺凝胶填充毛细管
公开(公告)号：US5141612A
公开(公告)日：1992-08-25
申请号：US626603
申请日：1990-12-12
申请人： Gerhard Schomburg , Jurgen A. Lux , Hong-Feng Yin
发明人： Gerhard Schomburg , Jurgen A. Lux , Hong-Feng Yin
IPC分类号： G01N27/447
CPC分类号： G01N27/44747 , G01N27/44704
摘要： Polyacrylamide gel filled silica capillaries of improved performance in electrophoresis are produced bya) leaching the capillary with a solution of a basic reagent;b) binding a substituted, double bond-containing silane to the silica surface of the capillary;c) binding an acrylamide to the treated surface and polymerizing the acrylamide to a linear polyacrylamide, eliminating electroosmotic flow and avoiding chemical bonding of the gel during its subsequent formation; andd) contacting the capillary with a solution of acrylamide/bisacrylamide and effecting crosslinking polymerization in the presence of a radical polymerization starter to form a gel.
摘要翻译：通过a）用碱性试剂的溶液浸提毛细管，产生具有改进的电泳性能的聚丙烯酰胺凝胶填充的二氧化硅毛细管; b）将取代的含双键硅烷与毛细管的二氧化硅表面结合; c）将丙烯酰胺结合到经处理的表面并将丙烯酰胺聚合成线性聚丙烯酰胺，消除电渗流并避免凝胶在其随后形成期间的化学键合; 和d）使毛细管与丙烯酰胺/双丙烯酰胺的溶液接触，并在自由基聚合引发剂的存在下进行交联聚合以形成凝胶。

7. 发明授权

US5262031A Electroosmotic flow control apparatus for capillary electrophoresis 失效
标题翻译：用于毛细管电泳的电渗流量控制装置
公开(公告)号：US5262031A
公开(公告)日：1993-11-16
申请号：US834174
申请日：1992-02-07
申请人： Jurgen A. Lux , Sally A. Swedberg , James E. Young , Douglass McManigill
发明人： Jurgen A. Lux , Sally A. Swedberg , James E. Young , Douglass McManigill
IPC分类号： G01N27/447 , G01N27/26
CPC分类号： G01N27/44713 , G01N27/44752
摘要： An electrophoretic apparatus that is useful for separation of analytes, such as proteins, comprises a capillary tube that has an interfacial layer bonded to reduce interactions between the bore and analytes during electroosmotic flow through the capillary, and an electrically modified media carried by or contacting a capillary wall outward from the bore. The modified media can be an electrically resistive coating or an electrically conductive coating. Together with appropriate power supply configurations, the electrically modified media permits the application of a voltage gradient that is transverse to the electrophoretic gradient, and provides electroosmotic flow control in cooperation with the interfacial layer.
摘要翻译：可用于分离分析物如蛋白质的电泳装置包括毛细管，其具有结合的界面层，以减少通过毛细管的电渗流过程中的孔和分析物之间的相互作用，以及由毛细管壁从孔内向外。改性介质可以是电阻涂层或导电涂层。与适当的电源配置一起，电修改的介质允许施加横向于电泳梯度的电压梯度，并且与界面层协作提供电渗流控制。

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