专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20140018257A1 Peptide Library Production Method, Peptide Library, and Screening Method 审中-公开
标题翻译：肽库生产方法，肽库和筛选方法
公开(公告)号：US20140018257A1
公开(公告)日：2014-01-16
申请号：US13990180
申请日：2011-12-05
申请人： Hiroaki Suga , Jumpei Morimoto
发明人： Hiroaki Suga , Jumpei Morimoto
IPC分类号： B01J19/00
CPC分类号： B01J19/0046 , B01J2219/00725 , C07K7/08 , C07K14/705 , C12N15/1062 , C12N15/67 , C12Q1/34 , C40B50/06 , C40B50/08
摘要： Object is to provide a method of constructing a library of peptides having, at a desired position in the random sequence of each peptide, an amino acid having a portion capable of binding to a target. The invention provides a method of producing a library of peptides having, at a designated position in the random sequence of each peptide, a special amino acid having a portion capable of binding to a target, including (i) preparing a library of mRNAs having, in the mRNA sequence coding for a random amino acid sequence, a base sequence having an altered codon encoding the special amino acid, (ii) preparing an aminoacyl tRNA with the special amino acid linked to a tRNA encoded by the altered codon, and (iii) translating the mRNAs by using a cell-free translation system containing the aminoacyl tRNA to obtain a library of peptides having, in the random sequence thereof, a predetermined special amino acid.
摘要翻译：目的是提供构建肽的文库的方法，其在每个肽的随机序列中的期望位置具有具有能与靶标结合的部分的氨基酸。本发明提供一种生产肽文库的方法，其在每个肽的随机序列中的指定位置具有具有能与靶标结合的部分的特殊氨基酸，其包括（i）制备mRNA的文库，在编码随机氨基酸序列的mRNA序列中，具有编码特殊氨基酸的改变的密码子的碱基序列，（ii）用与被改变的密码子编码的tRNA连接的特殊氨基酸制备氨酰基tRNA，和（iii ）通过使用含有氨酰基tRNA的无细胞翻译系统来翻译mRNA，以获得其随机序列中具有预定特殊氨基酸的肽文库。

IPRDB

热门服务

关于我们

友情链接

联系方式