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    • 2. 发明申请
    • DNA FRAGMENT FOR IMPROVING TRANSLATION EFFICIENCY, AND RECOMBINANT VECTOR CONTAINING SAME
    • 用于改进翻译效率的DNA片段和包含其的重组载体
    • US20120255069A1
    • 2012-10-04
    • US13393345
    • 2010-08-25
    • Yun Jeong NaEun Hyun JeonEun Hye KwonYong Woo KimEun Ju SohnIn Hwan Hwang
    • Yun Jeong NaEun Hyun JeonEun Hye KwonYong Woo KimEun Ju SohnIn Hwan Hwang
    • A01H5/00A01H5/10C12N15/113A01H1/06C12N15/82C12N5/10
    • C12N15/67
    • The present invention relates to a DNA fragment for improving translation efficiency, and a recombinant vector containing the same, and more specifically, to a DNA fragment which comprises any one nucleotide sequence selected from the group consisting of SEQ ID NOs: 1-6, SEQ ID NOs: 8-10, SEQ ID NOs: 13 and 14 and SEQ ID NO: 16, and improves the translation efficiency of a heterologous protein placed in the downstream, and a recombinant vector containing the DNA fragment. The DNA fragment for improving translation efficiency according to the present invention and a recombinant vector containing the same can improve the translation of a heterologous protein in a transgenic plant. In addition, if a leader polynucleotide inducing the targeting to a particular cellular organelle of a plant is further linked to the recombinant vector in an operable manner, the heterologous protein is targeted to the specific cellular organelle and can be stably accumulated, thereby enabling the mass production of the heterologous protein from the plant.
    • 本发明涉及用于提高翻译效率的DNA片段,以及含有该DNA片段的重组载体,更具体地,涉及包含选自SEQ ID NO:1-6,SEQ ID NO:1-6中任一个核苷酸序列的DNA片段 ID NO:8-10,SEQ ID NO:13和14以及SEQ ID NO:16,并提高了放置在下游的异源蛋白质的翻译效率和含有该DNA片段的重组载体。 用于提高根据本发明的翻译效率的DNA片段和含有该DNA片段的重组载体可以改善转基因植物中异源蛋白质的翻译。 此外,如果诱导靶向植物的特定细胞器的靶向多核苷酸进一步以可操作的方式与重组载体连接,则异源蛋白质靶向特定细胞器官细胞并可稳定地积聚,从而使质量 从植物中产生异源蛋白质。