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1. 发明授权

US5792924A Biologically safe plant transformation system 失效
公开(公告)号：US5792924A
公开(公告)日：1998-08-11
申请号：US445606
申请日：1995-05-22
申请人： John I. Yoder , Michael W. Lassner
发明人： John I. Yoder , Michael W. Lassner
IPC分类号： A01H1/00 , A01H1/04 , A01H5/00 , C12N15/09 , C12N15/64 , C12N15/82 , C12Q1/68 , C12N15/00 , A01H1/06
CPC分类号： C12Q1/683 , C12N15/64 , C12N15/8202 , C12N15/8242 , C12N15/8286 , C12Q1/6827 , C12Q1/6895
摘要： This invention relates to methods for producing transgenic plants that contain a gene of interest and that are free of foreign ancillary nucleic acids. These methods allow for the production of plants which thus contain a desired gene, but which are free of vector sequences and/or marker sequences used to transform the plant. The method of transforming such plants calls for transforming the plants with a gene of interest by introduction of the gene on a DNA construct comprising a transposon and foreign ancillary nucleic acids; crossing the transformed plant through self-crossing or with another plant to obtain F.sub.1 or more removed generation progeny; and utilizing a means for selecting those progeny that carry the gene of interest and are free of the ancillary nucleic acids. Such progeny may be detected biochemically, by Southern hybridization, through the use of polymerase chain reaction procedures and other methods available in the art.

2. 发明授权

US5482852A Biologically safe plant transformation system 失效
标题翻译：生物安全植物转化系统
公开(公告)号：US5482852A
公开(公告)日：1996-01-09
申请号：US77787
申请日：1993-06-15
申请人： John I. Yoder , Michael W. Lassner
发明人： John I. Yoder , Michael W. Lassner
IPC分类号： A01H1/00 , A01H1/04 , A01H5/00 , C12N15/09 , C12N15/64 , C12N15/82 , C12Q1/68 , C12N15/63 , A01H4/00
CPC分类号： C12Q1/683 , C12N15/64 , C12N15/8202 , C12N15/8242 , C12N15/8286 , C12Q1/6827 , C12Q1/6895
摘要： This invention relates to methods for producing transgenic plants that contain a gene of interest and that are free of foreign ancillary nucleic acids. These methods allow for the production of plants which thus contain a desired gene, but which are free of vector sequences and/or marker sequences used to transform the plant. The method of transforming such plants calls for transforming the plants with a gene of interest by introduction of the gene on a DNA construct comprising a transposon and foreign ancillary nucleic acids; crossing the transformed plant through self-crossing or with another plant to obtain F.sub.1 or more removed generation progeny; and utilizing a means for selecting those progeny that carry the gene of interest and are free of the ancillary nucleic acids. Such progeny may be detected biochemically, by Southern hybridization, through the use of polymerase chain reaction procedures and other methods available in the art.
摘要翻译：本发明涉及生产含有目的基因并且不含外来辅助核酸的转基因植物的方法。这些方法允许生产因此含有所需基因但不含用于转化植物的载体序列和/或标记序列的植物。转化这种植物的方法要求通过在包含转座子和外来辅助核酸的DNA构建体上引入基因来转化具有感兴趣基因的植物; 通过自交或与另一种植物交叉转化的植物以获得F1或更多的去除的代后代; 并利用一种用于选择携带目的基因并且不含辅助核酸的后代的方法。可以通过Southern杂交，通过使用聚合酶链反应方法和本领域可获得的其它方法在生物化学上检测这样的后代。

3. 发明授权

US5225341A Biologically safe plant transformation system using a Ds transposon 失效
标题翻译：使用DS传感器的生物安全植物转化系统
公开(公告)号：US5225341A
公开(公告)日：1993-07-06
申请号：US555271
申请日：1990-07-19
申请人： John I. Yoder , Michael W. Lassner
发明人： John I. Yoder , Michael W. Lassner
IPC分类号： A01H1/00 , A01H1/04 , A01H5/00 , C12N15/09 , C12N15/64 , C12N15/82 , C12Q1/68
CPC分类号： C12Q1/683 , C12N15/64 , C12N15/8202 , C12N15/8242 , C12N15/8286 , C12Q1/6827 , C12Q1/6895
摘要： This invention relates to methods for producing transgenic plants that contain a gene of interest and that are free of foreign ancillary nucleic acids. These methods allow for the production of plants which thus contain a desired gene, but which are free of vector sequences and/or marker sequences used to transform the plant. The method of transforming such plants calls for transforming the plants with a gene of interest by introduction of the gene on a DNA construct comprising a transposon and foreign ancillary nucleic acids; crossing the transformed plant through self-crossing or with another plant to obtain F.sub.1 or more removed generation progeny; and utilizing a means for selecting those progeny that carry the gene of interest and are free of the ancillary nucleic acids. Such progeny may be detected biochemically, by Southern hybridization, through the use of polymerase chain reaction procedures and other methods available in the art.
摘要翻译：本发明涉及生产含有目的基因并且不含外来辅助核酸的转基因植物的方法。这些方法允许生产因此含有所需基因但不含用于转化植物的载体序列和/或标记序列的植物。转化这种植物的方法要求通过在包含转座子和外来辅助核酸的DNA构建体上引入基因来转化具有感兴趣基因的植物; 通过自交或与另一种植物交叉转化的植物以获得F1或更多的去除的代后代; 并利用一种用于选择携带目的基因并且不含辅助核酸的后代的方法。可以通过Southern杂交，通过使用聚合酶链反应方法和本领域可获得的其它方法在生物化学上检测这样的后代。

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