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    • 5. 发明授权
    • Manipulation of SNF1 kinase for altered oil content in oleaginous organisms
    • 操纵SNF1激酶以改善含油生物体内油含量
    • US08435758B2
    • 2013-05-07
    • US12549439
    • 2009-08-28
    • Seung-Pyo HongQuinn Qun ZhuJohn E. Seip
    • Seung-Pyo HongQuinn Qun ZhuJohn E. Seip
    • C12P1/00C07H21/04C12N1/15
    • C12P7/6472C12P7/6427C12P7/6463
    • Methods of increasing the total lipid content in a eukaryotic cell, the total content of polyunsaturated fatty acids [“PUFAs”], and/or the ratio of desaturated fatty acids to saturated fatty acids by reducing the activity of the heterotrimeric SNF1 protein kinase are disclosed. Preferably, the chromosomal genes encoding the Snf1 α-subunit, Gal83 β-subunit or Snf4 γ-subunit of the SNF1 protein kinase, the upstream regulatory genes encoding Sak1, Hxk2, Glk1 or Reg1, or the downstream genes encoding Rme1, Cbr1 or Snf3 are manipulated in a PUFA-producing strain of the oleaginous yeast Yarrowia lipolytica, resulting in increased total lipid content, as compared to the parent strain comprising the heterotrimeric SNF1 protein kinase not having reduced activity.
    • 公开了通过降低异源三聚SNF1蛋白激酶的活性来增加真核细胞中总脂质含量,多不饱和脂肪酸的总含量[“PUFA”]和/或去饱和脂肪酸与饱和脂肪酸的比例的方法 。 优选地,编码SNF1蛋白激酶的Snf1α-亚基,Gal83β-亚基或Snf4γ-亚基的染色体基因,编码Sak1,Hxk2,Glk1或Reg1的上游调控基因或编码Rme1,Cbr1或Snf3的下游基因 与含有不具有降低的活性的异源三聚SNF1蛋白激酶的亲本菌株相比,在含油酵母解脂耶氏酵母解脂耶氏酵母解脂耶氏酵母的PUFA生产菌株中操作,导致总脂质含量增加。