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1. 发明授权

US6083712A Biotechnological method of producing biotin 失效
标题翻译：生物素生物技术方法
公开(公告)号：US6083712A
公开(公告)日：2000-07-04
申请号：US411768
申请日：1995-06-08
申请人： Olwen Birch , Johann Brass , Martin Fuhrmann , Nicholas Shaw
发明人： Olwen Birch , Johann Brass , Martin Fuhrmann , Nicholas Shaw
IPC分类号： C12N15/09 , C07K14/195 , C12N1/21 , C12N9/00 , C12N15/52 , C12N15/67 , C12P17/18 , C12P21/02 , C12R1/19 , C12P21/06 , C07H17/00 , C12N15/00
CPC分类号： C12P17/186 , C12N15/52 , C12N15/67
摘要： In DNA fragments and plasmids comprising the bioB, bioF, bioC, bioD and bioA genes responsible for biosynthesis of biotin, or their functionally equivalent genetic variants and mutants from enteric bacteria, the genes are arranged in a transcription unit. These DNA fragments and plasmids can be contained in microorganisms which can be used to produce biotin.
摘要翻译： PCT No.PCT / EP93 / 02688 Sec。 371日期：1995年6月8日 102（e）日期1995年6月8日PCT提交1993年10月1日PCT公布。公开号WO94 / 08023 日期1994年04月14日在包含负责生物素生物合成的bioB，bioF，bioC，bioD和bioA基因的DNA片段和质粒中，或其功能上等同的遗传变异体和来自肠细菌的突变体，将基因排列在转录单位中。这些DNA片段和质粒可以包含在可用于生产生物素的微生物中。

2. 发明授权

US09279128B2 Rhamnose promoter expression system 有权
标题翻译：鼠李糖启动子表达系统
公开(公告)号：US09279128B2
公开(公告)日：2016-03-08
申请号：US13317293
申请日：2011-10-14
申请人： Johann Brass , Christoph Kiziak , Joachim Klein , Ralf Ostendorp
发明人： Johann Brass , Christoph Kiziak , Joachim Klein , Ralf Ostendorp
IPC分类号： C12N15/10 , C12N15/00 , C12N15/70 , C07H21/02 , C12N15/63 , C12N15/78
CPC分类号： C12N15/78 , C07K16/00 , C07K2317/14 , C07K2317/55 , C07K2317/622 , C12N15/63 , C12N15/70
摘要： Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is: a) a nucleic acid sequence which is heterologous to the host, and b) a prokaryotic signal sequence operably linked to the nucleic acid sequence. The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.
摘要翻译：在宿主中可表达的是可操作地连接到转录单位的L-鼠李糖操纵子的rhaBAD启动子区的载体，所述转录单位是：a）与宿主异源的核酸序列，和b）可操作地连接到宿主的原核信号序列核酸序列。原核信号序列选自用于糖，氨基酸，维生素和离子的周质结合蛋白的信号肽。核酸序列的表达由启动子区域控制。该载体用于原核宿主中核酸序列的调节异源表达。这是在宿主中可表达的分离和纯化的核酸序列是L-鼠李糖操纵子的启动子区。存在使用载体在宿主中产生多肽的方法。

3. 发明申请

US20120135463A1 Rhamnose promoter expression system 审中-公开
标题翻译：鼠李糖启动子表达系统
公开(公告)号：US20120135463A1
公开(公告)日：2012-05-31
申请号：US13317293
申请日：2011-10-14
申请人： Johann Brass , Christoph Kiziak , Joachim Klein , Ralf Ostendorp
发明人： Johann Brass , Christoph Kiziak , Joachim Klein , Ralf Ostendorp
IPC分类号： C12P21/06 , C12N1/21 , C07H21/04 , C12N15/74 , C12N15/70 , C12N15/75 , C12N15/78
CPC分类号： C12N15/78 , C07K16/00 , C07K2317/14 , C07K2317/55 , C07K2317/622 , C12N15/63 , C12N15/70
摘要： Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is: a) a nucleic acid sequence which is heterologous to the host, and b) a prokaryotic signal sequence operably linked to the nucleic acid sequence. The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.
摘要翻译：在宿主中可表达的是可操作地连接到转录单位的L-鼠李糖操纵子的rhaBAD启动子区的载体，所述转录单位是：a）与宿主异源的核酸序列，和b）可操作地连接到宿主的原核信号序列核酸序列。原核信号序列选自用于糖，氨基酸，维生素和离子的周质结合蛋白的信号肽。核酸序列的表达由启动子区域控制。该载体用于原核宿主中核酸序列的调节异源表达。这是在宿主中可表达的分离和纯化的核酸序列是L-鼠李糖操纵子的启动子区。存在使用载体在宿主中产生多肽的方法。

4. 发明授权

US08735099B2 Melibiose operon expression system 有权
标题翻译： Melibiose操纵子表达系统
公开(公告)号：US08735099B2
公开(公告)日：2014-05-27
申请号：US13064928
申请日：2011-04-27
申请人： Johann Brass , Joachim Klein , Ralf Ostendorp
发明人： Johann Brass , Joachim Klein , Ralf Ostendorp
IPC分类号： C12P21/02 , C12N15/00 , C07H21/04
CPC分类号： C07K14/70 , C07K16/00 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C12N15/52
摘要： New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.
摘要翻译：可以在包含与包含与宿主异源的核酸序列的转录单位可操作地连接的蜜二糖操纵子的启动子区的宿主中表达的新载体。核酸序列的表达由蜜二糖操纵子的启动子区域控制。新载体可用于原核宿主中核酸序列的调节异源表达。存在在宿主中可表达的分离和纯化的核酸序列，其包含可操作地连接到包含与宿主异源的核酸序列的转录单位的蜜二糖操纵子的启动子区。核酸序列的表达由蜜二糖操纵子的启动子区域控制。用载体或分离和纯化的核酸序列转化原核宿主。存在使用载体在宿主中产生多肽的方法。

5. 发明申请

US20080305526A1 Melibiose Operon Expression System 审中-公开
标题翻译： Melibiose操纵子表达系统
公开(公告)号：US20080305526A1
公开(公告)日：2008-12-11
申请号：US11791988
申请日：2005-12-05
申请人： Johann Brass , Joachim Klein , Ralf Ostendorp
发明人： Johann Brass , Joachim Klein , Ralf Ostendorp
IPC分类号： C12P21/04 , C12N15/00 , C12N15/11 , C12N1/20
CPC分类号： C07K14/70 , C07K16/00 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C12N15/52
摘要： New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.
摘要翻译：可以在包含与包含与宿主异源的核酸序列的转录单位可操作地连接的蜜二糖操纵子的启动子区的宿主中表达的新载体。核酸序列的表达由蜜二糖操纵子的启动子区域控制。新载体可用于原核宿主中核酸序列的调节异源表达。存在在宿主中可表达的分离和纯化的核酸序列，其包含可操作地连接到包含与宿主异源的核酸序列的转录单位的蜜二糖操纵子的启动子区。核酸序列的表达由蜜二糖操纵子的启动子区域控制。用载体或分离和纯化的核酸序列转化原核宿主。存在使用载体在宿主中产生多肽的方法。

6. 发明申请

US20120077225A1 Melibiose operon expression system 有权
标题翻译： Melibiose操纵子表达系统
公开(公告)号：US20120077225A1
公开(公告)日：2012-03-29
申请号：US13064928
申请日：2011-04-27
申请人： Johann Brass , Joachim Klein , Ralf Ostendorp
发明人： Johann Brass , Joachim Klein , Ralf Ostendorp
IPC分类号： C12P21/00 , C12N1/00 , C12N15/63
CPC分类号： C07K14/70 , C07K16/00 , C07K2317/55 , C07K2317/622 , C07K2319/00 , C12N15/52
摘要： New vectors expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. The new vector can be used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. There is an isolated and purified nucleic acid sequence expressible in a host comprising the promoter region of the melibiose operon operably linked to a transcriptional unit that includes a nucleic acid sequence which is heterologous to the host. The expression of the nucleic acid sequence is controlled by the promoter region of the melibiose operon. A prokaryotic host is transformed with the vector or the isolated and purified nucleic acid sequence. There is a method for producing a polypeptide in a host using the vector.
摘要翻译：可以在包含与包含与宿主异源的核酸序列的转录单位可操作地连接的蜜二糖操纵子的启动子区的宿主中表达的新载体。核酸序列的表达由蜜二糖操纵子的启动子区域控制。新载体可用于原核宿主中核酸序列的调节异源表达。存在在宿主中可表达的分离和纯化的核酸序列，其包含可操作地连接到包含与宿主异源的核酸序列的转录单位的蜜二糖操纵子的启动子区。核酸序列的表达由蜜二糖操纵子的启动子区域控制。用载体或分离和纯化的核酸序列转化原核宿主。存在使用载体在宿主中产生多肽的方法。

7. 发明申请

US20080206817A1 Rhamnose Promoter Expression System 审中-公开
标题翻译：鼠李糖启动子表达系统
公开(公告)号：US20080206817A1
公开(公告)日：2008-08-28
申请号：US11791989
申请日：2005-12-05
申请人： Johann Brass , Christoph Kiziak , Joachim Klein , Ralf Ostendorp
发明人： Johann Brass , Christoph Kiziak , Joachim Klein , Ralf Ostendorp
IPC分类号： C12P21/00 , C12N15/63 , C12N15/00 , C07H21/00 , C12N1/00
CPC分类号： C12N15/78 , C07K16/00 , C07K2317/14 , C07K2317/55 , C07K2317/622 , C12N15/63 , C12N15/70
摘要： Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is: a) a nucleic acid sequence which is heterologouse to the host, and b) a prokaryotic signal sequence operably linked to the nucleic acid sequence. The prokaryotic signal sequence is selected from signal peptides of periplasmatic binding proteins for sugars, amino acids, vitamins and ions. The expression of the nucleic acid sequence is controlled by the promoter region. The vector is used for the regulated heterologous expression of a nucleic acid sequence in a prokaryotic host. This is an isolated and purified nucleic acid sequence expressible in a host is the promoter region of the L-rhamnose operon. There is a method for producing a polypeptide in a host using the vector.
摘要翻译：在宿主中可表达的是可操作地连接到转录单位的L-鼠李糖操纵子的rhaBAD启动子区的载体，所述转录单位是：a）与宿主异源的核酸序列，和b）可操作地连接到宿主的原核信号序列核酸序列。原核信号序列选自用于糖，氨基酸，维生素和离子的周质结合蛋白的信号肽。核酸序列的表达由启动子区域控制。该载体用于原核宿主中核酸序列的调节异源表达。这是在宿主中可表达的分离和纯化的核酸序列是L-鼠李糖操纵子的启动子区。存在使用载体在宿主中产生多肽的方法。

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