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1. 发明授权

US08735085B2 Method for diagnosing diseases using a protein or degradation product based on half-life 有权
标题翻译：基于半衰期使用蛋白质或降解产物诊断疾病的方法
公开(公告)号：US08735085B2
公开(公告)日：2014-05-27
申请号：US12287059
申请日：2008-10-03
申请人： Craig A. Gelfand , Jizu Yi , Gang Ju
发明人： Craig A. Gelfand , Jizu Yi , Gang Ju
IPC分类号： C12Q1/56
CPC分类号： G01N33/68 , G01N33/86 , G01N2333/75
摘要： One aspect of the invention provides a method of diagnosing a disease condition, comprising measuring presence or amount of a targeted protein or a degradation product of said protein in a collected biological sample as a marker for the disease condition. The targeted protein or degradation product is selected for measurement based on a prior identification of a measurable half-life at a predetermined time period, including the time at which said method is conducted, and correlating said measuring with the presence or absence of the disease condition. The targeted protein or degradation product may be identified by selecting a protein known or suspected to be a diagnostic marker for the disease condition, analyzing degradation of the protein in the collected biological sample, and selecting a protein or degradation product that exhibits a measurable half-life at a predetermined period of time. The analyzing may include identifying degradation product(s) of the protein as a function of time, and half-life of the protein and the degradation product(s).
摘要翻译：本发明的一个方面提供了一种诊断疾病状况的方法，包括测定收集的生物样品中所述蛋白质的靶向蛋白质或降解产物的存在或量作为疾病状况的标志物。基于在预定时间段（包括进行所述方法的时间）的可测量的半衰期的先前识别，并将所述测量与存在或不存在疾病状态相关联，选择靶向蛋白质或降解产物进行测量。可以通过选择已知或怀疑是疾病状况的诊断标记物的蛋白质或分解产物来分析靶向蛋白质或降解产物，分析所收集的生物样品中蛋白质的降解，以及选择显示可测量的半衰期的蛋白质或降解产物，寿命在预定的时间。分析可以包括鉴定蛋白质的降解产物作为时间的函数，以及蛋白质和降解产物的半衰期。

2. 发明申请

US20080017577A1 Membrane-based Double-layer Tube for Sample Collections 审中-公开
标题翻译：用于样品集合的膜基双层管
公开(公告)号：US20080017577A1
公开(公告)日：2008-01-24
申请号：US11459076
申请日：2006-07-21
申请人： Jizu Yi , Fu Chung Lin , Dimitrios Manoussakis , Craig Gelfand
发明人： Jizu Yi , Fu Chung Lin , Dimitrios Manoussakis , Craig Gelfand
IPC分类号： B01D61/18
CPC分类号： B01L3/50825 , B01L3/502 , B01L3/5021 , B01L2300/047 , B01L2300/0672 , B01L2300/0681 , B01L2400/049
摘要： The fluid sample collection device is adapted to collect and separate a fluid sample into constituent parts such as separating plasma or serum from a blood sample. The device includes an evacuated outer container and an inner container. The outer container has a first open end and a second closed end. A pierceable closure closes the first open end thereby defining a first interior chamber. The inner container is contained within the outer container and separates the first interior chamber into an upper chamber portion and lower chamber portion in fluid communication. The inner container defines a second interior chamber separated from the lower chamber portion through a porous membrane. A port is provided for placing the second interior chamber in fluid communication with the first interior chamber. Another aspect of the device relates to a method of using the device to separate plasma or serum from a blood sample.
摘要翻译：流体样品收集装置适于将流体样品收集并分离成组成部分，例如从血液样品分离血浆或血清。该装置包括抽真空的外部容器和内部容器。外容器具有第一开口端和第二闭合端。可刺破的封闭件封闭第一开口端，由此限定第一内部腔室。内部容器被容纳在外部容器内并且将第一内部腔室分离成上部腔室部分和下部腔室部分，以使其流体连通。内部容器限定通过多孔膜与下部室分隔开的第二内部室。提供端口用于将第二内部腔室与第一内部腔室流体连通。该装置的另一方面涉及使用该装置将血浆或血清与血液样品分离的方法。

3. 发明申请

US20090208923A1 System and method for diagnosing diseases 有权
标题翻译：诊断疾病的系统和方法
公开(公告)号：US20090208923A1
公开(公告)日：2009-08-20
申请号：US12287059
申请日：2008-10-03
申请人： Craig A. Gelfand , Jizu Yi , Gang Ju
发明人： Craig A. Gelfand , Jizu Yi , Gang Ju
IPC分类号： C12Q1/00
CPC分类号： G01N33/68 , G01N33/86 , G01N2333/75
摘要： One aspect of the invention provides a method of diagnosing a disease condition, comprising measuring presence or amount of a targeted protein or a degradation product of said protein in a collected biological sample as a marker for the disease condition. The targeted protein or degradation product is selected for measurement based on a prior identification of a measurable half-life at a predetermined time period, including the time at which said method is conducted, and correlating said measuring with the presence or absence of the disease condition. The targeted protein or degradation product may be identified by selecting a protein known or suspected to be a diagnostic marker for the disease condition, analyzing degradation of the protein in the collected biological sample, and selecting a protein or degradation product that exhibits a measurable half-life at a predetermined period of time. The analyzing may include identifying degradation product(s) of the protein as a function of time, and half-life of the protein and the degradation product(s).
摘要翻译：本发明的一个方面提供了一种诊断疾病状况的方法，包括测定收集的生物样品中所述蛋白质的靶向蛋白质或降解产物的存在或量作为疾病状况的标志物。基于在预定时间段（包括进行所述方法的时间）的可测量的半衰期的先前识别，并将所述测量与存在或不存在疾病状态相关联，选择靶向蛋白质或降解产物进行测量。可以通过选择已知或怀疑是疾病状况的诊断标记物的蛋白质或分解产物来分析靶向蛋白质或降解产物，分析所收集的生物样品中蛋白质的降解，以及选择显示可测量的半衰期的蛋白质或降解产物，寿命在预定的时间。分析可以包括鉴定蛋白质的降解产物作为时间的函数，以及蛋白质和降解产物的半衰期。

4. 发明授权

US07361489B2 Nucleic acid amplification methods 有权
标题翻译：核酸扩增方法
公开(公告)号：US07361489B2
公开(公告)日：2008-04-22
申请号：US10719480
申请日：2003-11-21
申请人： David Y. Zhang , Wandi Zhang , Jizu Yi
发明人： David Y. Zhang , Wandi Zhang , Jizu Yi
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12Q1/682 , C12Q1/6827 , C12Q1/6834 , C12Q1/6858 , C12Q2600/158 , C12Q2561/125 , C12Q2531/125 , C12Q2525/307 , C12Q2565/101 , C12Q2525/197 , C12Q2525/161 , C12Q2537/143 , C12Q2565/519 , C12Q2563/143 , C12Q2531/119 , C12Q2531/113
摘要： The present invention relates to assays and kits for carrying out said assays for the rapid, automated detection of infectious pathogenic agents and normal and abnormal genes. The present invention further relates to methods for general amplification of genomic DNA and total mRNAs and for analyzing differential mRNA expression using the amplification methods disclosed herein.
摘要翻译：本发明涉及用于进行用于快速，自动检测感染性病原体和正常和异常基因的所述测定的测定和试剂盒。本发明还涉及基因组DNA和总mRNA的一般扩增方法以及使用本文公开的扩增方法分析差异mRNA表达的方法。

5. 发明申请

US20050037353A1 Highly sensitive methods for protein detection in proteomics 审中-公开
标题翻译：蛋白质组学中蛋白质检测的高灵敏度方法
公开(公告)号：US20050037353A1
公开(公告)日：2005-02-17
申请号：US10492860
申请日：2002-10-15
申请人： David Zhang , Jizu Yi
发明人： David Zhang , Jizu Yi
IPC分类号： C12Q1/68 , C12Q1/6844 , G01N33/58 , G01N33/53 , C12P19/34
CPC分类号： B82Y15/00 , C12Q1/68 , C12Q1/6844 , G01N33/58 , G01N33/588 , C12Q2563/179 , C12Q2525/307
摘要： The present invention relates to methods for identification and quantification of proteins expressed within a cell. The methods of the invention involve the separation of proteins based on their physical properties such as, for example, net charge, molecular weight, or immunoreactivity, followed by detection of said proteins using a number of different techniques including (i) ramification-extension amplification method (RAM); (ii) hybridization signal amplification method (HSAM); and (iii) detection with nanodots. The methods of the invention will have a variety of different uses including, but not limited to, uses for screening, for diagnosis and prognosis of disease. The methods of the invention are especially useful for identification of proteins that are not easily identified due to the small size of the protein, low concentration of the protein, or failure to separate proteins due to similar physical properties.
摘要翻译：本发明涉及鉴定和定量细胞内表达的蛋白质的方法。本发明的方法包括基于它们的物理特性（例如净电荷，分子量或免疫反应性）分离蛋白质，然后使用许多不同的技术检测所述蛋白质，包括（i）分枝扩增扩增方法（RAM）; （ii）杂交信号扩增法（HSAM）; 和（iii）用纳米点检测。本发明的方法将具有各种不同的用途，包括但不限于筛选用途，疾病的诊断和预后。本发明的方法特别可用于鉴定由于蛋白质的小尺寸，蛋白质的低浓度或由于相似的物理性质而不能分离蛋白质而不容易鉴别的蛋白质。

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