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    • 4. 发明授权
    • Modulation of cytokinin activity in plants
    • 植物中细胞分裂素活性的调节
    • US07531723B2
    • 2009-05-12
    • US10817483
    • 2004-04-02
    • Jeffrey E. HabbenChris ZinselmeierDwight T. TomesTimothy G. Helentjaris
    • Jeffrey E. HabbenChris ZinselmeierDwight T. TomesTimothy G. Helentjaris
    • C12N15/82A01H5/00A01H5/10
    • C12N15/8234C12N9/0032C12N9/1085C12N15/8243C12N15/8261C12N15/8271C12N15/8295Y02A40/146
    • This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and/or spatially-regulated expression of genes that affect metabolically effective levels of cytokinins in plants, particularly in plant seeds and related female reproductive tissue. This invention further relates to transgenic plants having enhanced levels of cytokinin expression wherein the transgenic plant exhibits useful characteristics, such as improved seed size, decreased tip kernel abortion, increased seed set during unfavorable environmental conditions, or stability of yield. The present invention also provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions comprise novel nucleotide sequences for seed-preferred promoters known as eep1 and eep2. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
    • 本发明一般涉及植物分子生物学领域。 更具体地说,本发明涉及影响植物中特别是植物种子和相关雌性生殖组织中细胞分裂素的代谢有效水平的基因的时间和/或空间调节表达的方法和试剂。 本发明进一步涉及具有增强的细胞分裂素表达水平的转基因植物,其中转基因植物表现出有用的特征,例如改善的种子大小,降低的末梢核仁流产,在不利的环境条件下增加的种子集合或产量的稳定性。 本发明还提供用于调节植物中异源核苷酸序列表达的组合物和方法。 组合物包含已知为eep1和eep2的种子优选启动子的新型核苷酸序列。 提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。 该方法包括转化植物细胞以包含与本发明的启动子之一可操作地连接的异源核苷酸序列,并从转化的植物细胞再生稳定转化的植物。
    • 10. 发明授权
    • Methods to increase yield of plants grown at high populations
    • 增加种群生长的植物产量的方法
    • US08250807B2
    • 2012-08-28
    • US12852598
    • 2010-08-09
    • Jeffrey E. Habben
    • Jeffrey E. Habben
    • A01G1/00
    • A01G2/00
    • An increase in the total grain and biomass yields and photosynthetic rates of plants grown at high densities achieved by novel methods of defoliating the primary-source leaf of a majority of such plants. In particular the methods provide for increased grain and biomass yields, including for example, increased average kernel mass for maize plants, and photosynthetic rates through the timely removal of the primary-source leaf of a majority of the plants grown at high densities. This invention also relates to seeds obtained by using the disclosed methods.
    • 通过对大多数这样的植物的主要来源叶进行脱叶的新方法,在高密度下生长的植物的总谷物和生物量产量和光合速率的增加。 特别地,这些方法提供了增加的谷物和生物量产量,包括例如玉米植物的平均籽粒质量增加,以及通过及时去除高密度生长的大多数植物的主要来源叶的光合速率。 本发明还涉及通过使用所公开的方法获得的种子。