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1. 发明申请

US20100184017A1 DIFFERENTIAL AMPLIFICATION OF MUTANT NUCLEIC ACIDS BY PCR IN A MIXTURE OF NUCLEIC ACIDS 审中-公开
标题翻译：通过PCR在核酸混合物中的突变核酸的差异放大
公开(公告)号：US20100184017A1
公开(公告)日：2010-07-22
申请号：US12691377
申请日：2010-01-21
申请人： SIMON WALN-HOBSON , JEAN-PIERRE VARTANIAN
发明人： SIMON WALN-HOBSON , JEAN-PIERRE VARTANIAN
IPC分类号： C12Q1/70 , C12P19/34 , C12Q1/68 , G01N33/559
CPC分类号： C12P19/34 , C12Q1/6827 , C12Q2537/143 , C12Q2531/113 , C12Q2527/107
摘要： A method for enriching a mutant nucleic acid in a mixture of nucleic acids, wherein the method comprises: (a) providing a nucleic acid mixture comprising a parental nucleic acid and a mutant nucleic acid of the parental nucleic acid; and (b) amplifying the nucleic acids in the nucleic acid mixture by polymerase chain reaction (PCR); wherein the mutant nucleic acid is a G→A mutant of the parental nucleic acid, which pairs with a fully complementary nucleic acid sequence to form an AT-rich nucleic acid variant of the parental nucleic acid; and wherein the AT-rich nucleic acid variant is denatured and selectively amplified by carrying out PCR using a denaturation temperature 1-3° C. lower than the lowest denaturation temperature (Tp) that allows amplification of the parental nucleic acid to thereby enrich the mutant nucleic acid in the nucleic acid mixture.
摘要翻译：一种用于在核酸混合物中富集突变核酸的方法，其中所述方法包括：（a）提供包含亲本核酸和亲核酸的突变核酸的核酸混合物; 和（b）通过聚合酶链式反应（PCR）扩增核酸混合物中的核酸; 其中所述突变核酸是亲本核酸的G→A突变体，其与完全互补的核酸序列配对以形成亲本核酸的富含AT的核酸变体; 并且其中富含AT的核酸变体通过进行PCR而变性并选择性扩增，所述变性温度比最低变性温度（Tp）低1-3℃，所述变性温度允许扩增亲本核酸从而使突变体核酸混合物中的核酸。

2. 发明申请

US20120073003A1 APOBEC3 MEDIATED DNA EDITING 审中-公开
标题翻译： APOBEC3介导的DNA编码
公开(公告)号：US20120073003A1
公开(公告)日：2012-03-22
申请号：US13103727
申请日：2011-05-09
申请人： Simon Wain-Hobson , Jean-Pierre Vartanian
发明人： Simon Wain-Hobson , Jean-Pierre Vartanian
IPC分类号： C12Q1/70 , A01K67/027 , G01N33/573 , C12Q1/34 , C12Q1/68 , C07H21/04 , C12N5/071
CPC分类号： C12Q1/34 , G01N33/5011 , G01N2333/978 , G01N2500/02
摘要： The present invention relates to methods and compositions for preventing the occurance or progression of a cancer or pre-cancerous condition associated with expression, or over-expression of human cytidine deaminases of the APOBEC3 family. The invention also relates to drug screening assays designed to identify compounds that regulate the activity, or level of expression, of hA3A, hA3C and hA3H. The invention further relates to transgenic mice, as well as cells derived from said mice, that have been genetically engineered to express, or over-express hA3A, hA3C and/or hA3H. Such mice may be utilized to screen for, or identify, compounds that modulate the activity, or expression, of the human cytidine deaminases. The present invention also provides topical compositions such as cosmetic lotion, crème, or sunscreen for use on the skin, which comprise one or more inhibitors of human cytidine deaminase activity. The present invention relates to a double stranded DNA obtained following opening up of its duplex structure, said DNA being edited with cellular protein normally or abnormally expressed in the nucleus of an eukaryotic cell. The mono stranded DNA derived from the said double stranded DNA is a part of the present invention.
摘要翻译：本发明涉及用于预防与APOBEC3家族的人胞苷脱氨酶的表达或过度表达相关的癌症或癌前病症的发生或进展的方法和组合物。本发明还涉及设计用于鉴定调节hA3A，hA3C和hA3H的活性或表达水平的化合物的药物筛选测定。本发明进一步涉及转基因小鼠以及源自所述小鼠的细胞，其已被遗传工程化以表达或过表达hA3A，hA3C和/或hA3H。这些小鼠可用于筛选或鉴定调节人胞苷脱氨酶活性或表达的化合物。本发明还提供局部组合物，例如用于皮肤的化妆品乳液，奶油或防晒剂，其包含一种或多种人胞苷脱氨酶活性的抑制剂。本发明涉及在其双链体结构开放后获得的双链DNA，所述DNA通过在真核细胞核中正常或异常表达的细胞蛋白进行编辑。衍生自所述双链DNA的单链DNA是本发明的一部分。

3. 发明授权

US08288094B2 APOBEC3 mediated DNA editing 有权
标题翻译： APOBEC3介导的DNA编辑
公开(公告)号：US08288094B2
公开(公告)日：2012-10-16
申请号：US12100212
申请日：2008-04-09
申请人： Simon Wain-Hobson , Jean-Pierre Vartanian
发明人： Simon Wain-Hobson , Jean-Pierre Vartanian
IPC分类号： C12Q1/68
CPC分类号： C12Q1/34 , G01N33/5011 , G01N2333/978 , G01N2500/02
摘要： The present invention relates to methods and compositions for preventing the occurrence or progression of a cancer or pre-cancerous condition associated with expression, or over-expression of human cytidine deaminases of the APOBEC3 family. The invention also relates to drug screening assays designed to identify compounds that regulate the activity, or level of expression, of hA3A, hA3C and hA3H. The invention further relates to transgenic mice, as well as cells derived from said mice, that have been genetically engineered to express, or over-express hA3A, hA3C and/or hA3H. Such mice may be utilized to screen for, or identify, compounds that modulate the activity, or expression, of the human cytidine deaminases. The present invention also provides topical compositions such as cosmetic lotion, crème, or sunscreen for use on the skin, which comprise one or more inhibitors of human cytidine deaminase activity. The present invention relates to a double stranded DNA obtained following opening up of its duplex structure, said DNA being edited with cellular protein normally or abnormally expressed in the nucleus of an eukaryotic cell. The mono stranded DNA derived from the said double stranded DNA is a part of the present invention.
摘要翻译：本发明涉及用于预防与APOBEC3家族的人胞苷脱氨酶的表达或过度表达相关的癌症或癌前病症的发生或进展的方法和组合物。本发明还涉及设计用于鉴定调节hA3A，hA3C和hA3H的活性或表达水平的化合物的药物筛选测定。本发明进一步涉及转基因小鼠以及源自所述小鼠的细胞，其已被遗传工程化以表达或过表达hA3A，hA3C和/或hA3H。这些小鼠可用于筛选或鉴定调节人胞苷脱氨酶活性或表达的化合物。本发明还提供局部组合物，例如用于皮肤的化妆品乳液，奶油或防晒剂，其包含一种或多种人胞苷脱氨酶活性的抑制剂。本发明涉及在其双链体结构开放后获得的双链DNA，所述DNA通过在真核细胞核中正常或异常表达的细胞蛋白进行编辑。衍生自所述双链DNA的单链DNA是本发明的一部分。

4. 发明授权

US08541206B2 Differential amplification of mutant nucleic acids by PCR in a mixture of nucleic acids 失效
标题翻译：在核酸混合物中通过PCR差异扩增突变核酸
公开(公告)号：US08541206B2
公开(公告)日：2013-09-24
申请号：US12086897
申请日：2006-12-28
申请人： Simon Wain-Hobson , Jean-Pierre Vartanian
发明人： Simon Wain-Hobson , Jean-Pierre Vartanian
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6827 , C12Q2537/143 , C12Q2531/113 , C12Q2527/107
摘要： A method for enriching a mutant nucleic acid in a mixture of nucleic acids, wherein the method comprises: (a) providing a nucleic acid mixture comprising a parental nucleic acid and a mutant nucleic acid of the parental nucleic acid; and (b) amplifying the nucleic acids in the nucleic acid mixture by polymerase chain reaction (PCR); wherein the mutant nucleic acid is a G→A mutant of the parental nucleic acid, which pairs with a fully complementary nucleic acid sequence to form an AT-rich nucleic acid variant of the parental nucleic acid; and wherein the AT-rich nucleic acid variant is denatured and selectively amplified by carrying out PCR using a denaturation temperature 1-3° C. lower than the lowest denaturation temperature (Tp) that allows amplification of the parental nucleic acid to thereby enrich the mutant nucleic acid in the nucleic acid mixture.
摘要翻译：一种用于在核酸混合物中富集突变核酸的方法，其中所述方法包括：（a）提供包含亲本核酸和亲本核酸的突变核酸的核酸混合物; 和（b）通过聚合酶链式反应（PCR）扩增核酸混合物中的核酸; 其中所述突变核酸是所述亲本核酸的G→A突变体，其与完全互补的核酸序列配对以形成所述亲本核酸的富含AT的核酸变体; 并且其中富含AT的核酸变体通过进行PCR而变性并选择性扩增，所述变性温度比最低变性温度（Tp）低1-3℃，所述变性温度允许扩增亲本核酸从而使突变体核酸混合物中的核酸。

5. 发明申请

US20110003282A1 DIFFERENTIAL AMPLIFICATION OF MUTANT NUCLEIC ACIDS BY PCR IN A MIXTURE OF NUCLEIC ACIDS 失效
标题翻译：通过PCR在核酸混合物中的突变核酸的差异放大
公开(公告)号：US20110003282A1
公开(公告)日：2011-01-06
申请号：US12086897
申请日：2006-12-28
申请人： Simon Wain-Hobson , Jean-Pierre Vartanian
发明人： Simon Wain-Hobson , Jean-Pierre Vartanian
IPC分类号： C12Q1/68 , C12P19/34 , G01N27/26
CPC分类号： C12P19/34 , C12Q1/6827 , C12Q2537/143 , C12Q2531/113 , C12Q2527/107
摘要： A method for enriching a mutant nucleic acid in a mixture of nucleic acids, wherein the method comprises: (a) providing a nucleic acid mixture comprising a parental nucleic acid and a mutant nucleic acid of the parental nucleic acid; and (b) amplifying the nucleic acids in the nucleic acid mixture by polymerase chain reaction (PCR); wherein the mutant nucleic acid is a G→A mutant of the parental nucleic acid, which pairs with a fully complementary nucleic acid sequence to form an AT-rich nucleic acid variant of the parental nucleic acid; and wherein the AT-rich nucleic acid variant is denatured and selectively amplified by carrying out PCR using a denaturation temperature 1-3° C. lower than the lowest denaturation temperature (Tp) that allows amplification of the parental nucleic acid to thereby enrich the mutant nucleic acid in the nucleic acid mixture.
摘要翻译：一种用于在核酸混合物中富集突变核酸的方法，其中所述方法包括：（a）提供包含亲本核酸和亲本核酸的突变核酸的核酸混合物; 和（b）通过聚合酶链式反应（PCR）扩增核酸混合物中的核酸; 其中所述突变核酸是亲本核酸的G→A突变体，其与完全互补的核酸序列配对以形成亲本核酸的富含AT的核酸变体; 并且其中富含AT的核酸变体通过进行PCR而变性并选择性扩增，所述变性温度比最低变性温度（Tp）低1-3℃，所述变性温度允许扩增亲本核酸从而使突变体核酸混合物中的核酸。

6. 发明申请

US20090260090A1 APOBEC3 MEDIATED DNA EDITING 有权
标题翻译： APOBEC3介导的DNA编码
公开(公告)号：US20090260090A1
公开(公告)日：2009-10-15
申请号：US12100212
申请日：2008-04-09
申请人： Simon Wain-Hobson , Jean-Pierre Vartanian
发明人： Simon Wain-Hobson , Jean-Pierre Vartanian
IPC分类号： A61K49/00 , C12Q1/34 , C12N5/00 , A01K67/027 , C07H21/04
CPC分类号： C12Q1/34 , G01N33/5011 , G01N2333/978 , G01N2500/02
摘要： The present invention relates to methods and compositions for preventing the occurance or progression of a cancer or pre-cancerous condition associated with expression, or over-expression of human cytidine deaminases of the APOBEC3 family. The invention also relates to drug screening assays designed to identify compounds that regulate the activity, or level of expression, of hA3A, hA3C and hA3H. The invention further relates to transgenic mice, as well as cells derived from said mice, that have been genetically engineered to express, or over-express hA3A, hA3C and/or hA3H. Such mice may be utilized to screen for, or identify, compounds that modulate the activity, or expression, of the human cytidine deaminases. The present invention also provides topical compositons such as cosmetic lotion, crème, or sunscreen for use on the skin, which comprise one or more inhibitors of human cytidine deaminase activity. The present invention relates to a double stranded DNA obtained following opening up of its duplex structure, said DNA being edited with cellular protein normally or abnormally expressed in the nucleus of an eukaryotic cell. The mono stranded DNA derived from the said double stranded DNA is a part of the present invention.
摘要翻译：本发明涉及用于预防与APOBEC3家族的人胞苷脱氨酶的表达或过度表达相关的癌症或癌前病症的发生或进展的方法和组合物。本发明还涉及设计用于鉴定调节hA3A，hA3C和hA3H的活性或表达水平的化合物的药物筛选测定。本发明进一步涉及转基因小鼠以及源自所述小鼠的细胞，其已被遗传工程化以表达或过表达hA3A，hA3C和/或hA3H。这些小鼠可用于筛选或鉴定调节人胞苷脱氨酶活性或表达的化合物。本发明还提供局部组合物，例如用于皮肤的化妆品洗剂，奶油或防晒剂，其包含一种或多种人胞苷脱氨酶活性的抑制剂。本发明涉及在其双链体结构开放后获得的双链DNA，所述DNA通过在真核细胞核中正常或异常表达的细胞蛋白进行编辑。衍生自所述双链DNA的单链DNA是本发明的一部分。

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