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1. 发明授权

US5850023A Modified plant viral replicase genes 失效
标题翻译：改良植物病毒复制酶基因
公开(公告)号：US5850023A
公开(公告)日：1998-12-15
申请号：US967999
申请日：1997-11-12
申请人： James Scott Elmer , Daniel Nathans , Stephen Gary Rogers
发明人： James Scott Elmer , Daniel Nathans , Stephen Gary Rogers
IPC分类号： C07K14/01 , C12N9/12 , C12N15/82 , A01H1/04 , C07H21/04 , C12N15/00
CPC分类号： C12N9/1241 , C07K14/005 , C12N15/8283 , C12N2750/12022
摘要： DNA sequences encoding plant viral proteins which contain modifications of the amino acid sequence -Gly1-Xaa1-Xaa2-Xaa3-Xaa4-Gly2-Lys- are disclosed. These modified proteins confer viral resistance when expressed in transformed plants. A method for providing resistance to infection by a geminivirus in a susceptible plant are disclosed. Plants expressing the modified genes which are resistant to viral infection are also disclosed.
摘要翻译：公开了编码含有氨基酸序列-Gly1-Xaa1-Xaa2-Xaa3-Xaa4-Gly2-Lys-的修饰的植物病毒蛋白的DNA序列。这些修饰的蛋白质在转化植物中表达时赋予病毒抗性。公开了一种在敏感植物中提供双因素病毒感染抵抗力的方法。还公开了表达抗病毒感染的修饰基因的植物。

2. 发明授权

US5898096A Plant promoter 失效
标题翻译：植物启动子
公开(公告)号：US5898096A
公开(公告)日：1999-04-27
申请号：US717672
申请日：1996-09-23
申请人： Harry John Klee , James Scott Elmer
发明人： Harry John Klee , James Scott Elmer
IPC分类号： C07K14/415 , C12N15/82 , C12N15/00 , A01H4/00 , C12N15/29
CPC分类号： C07K14/415 , C12N15/8229 , C12N15/8275
摘要： A plant promoter that is a nucleic acid region located upstream of the 5' end of a plant DNA structural coding sequence that is transcribed at high levels in meristematic tissue and/or rapidly dividing cells. This promoter region is capable of conferring high levels of transcription in meristematic tissue and/or rapidly dividing cells when used as a promoter for a heterologous coding sequence in a chimeric gene. The promoter and any chimeric gene in which it may be used can be used to obtain transformed plants or plant cells. A DNA coding sequence that codes for a gene that is highly transcribed in meristematic tissue of Arabidopsis thaliana is also disclosed. This coding sequence can be used to obtain a cDNA probe useful in obtaining analogous promoters from a homologous coding sequence in other plant species. Chimeric genes including the isolated promoter region, transformed plants containing the isolated promoter region, transformed plant cells and seeds are also disclosed.
摘要翻译：植物启动子，其是位于分生组织和/或快速分裂细胞中以高水平转录的植物DNA结构编码序列的5'末端上游的核酸区域。当用作嵌合基因中的异源编码序列的启动子时，该启动子区域能够赋予分生组织和/或快速分裂细胞中高水平的转录。可以使用其可以使用的启动子和任何嵌合基因来获得转化的植物或植物细胞。还公开了编码在拟南芥的分生组织中高度转录的基因的DNA编码序列。该编码序列可用于获得可用于从其他植物物种中的同源编码序列获得类似启动子的cDNA探针。还公开了包括分离的启动子区域的嵌合基因，含有分离的启动子区域的转化植物，转化的植物细胞和种子。

3. 发明授权

US6147278A Plant vectors 失效
标题翻译：植物载体
公开(公告)号：US6147278A
公开(公告)日：2000-11-14
申请号：US261770
申请日：1999-03-03
申请人： Stephen G. Rogers , Leslie Brand , Robert B. Horsch , Robert T. Fraley , James Scott Elmer , David Bisaro
发明人： Stephen G. Rogers , Leslie Brand , Robert B. Horsch , Robert T. Fraley , James Scott Elmer , David Bisaro
IPC分类号： C12N15/34 , C12N15/82 , C12N15/83 , C12N15/84
CPC分类号： C12N15/8203
摘要： The invention relates to novel plant plasmid vectors comprising geminivirus DNA or a portion thereof having inserted therein a heterologous DNA sequence or gene, to processes and DNA intermediates useful in producing said vectors and to methods utilizing such vectors to replicate and express heterologous DNA sequences or genes in plants. In some embodiments, methods and compositions are provided for Ti plasmid delivery of these novel vectors into plants. In other embodiments, methods and compositions are provided which allow for the generation of geminivirus DNA containing plant plasmids in stably transformed plants. In still other embodiments, methods and compositions are provided for replicating and expressing heterologous DNA sequences or genes in plants employing the geminivirus DNA containing vectors of the present invention without causing disease symptoms.
摘要翻译：本发明涉及包含双子病毒DNA或其中插入有异源DNA序列或基因的部分的新型植物质粒载体，可用于生产所述载体的方法和DNA中间体，以及利用此类载体复制和表达异源DNA序列或基因的方法在植物中。在一些实施方案中，提供了将这些新载体的Ti质粒递送到植物中的方法和组合物。在其它实施方案中，提供了允许在稳定转化的植物中产生含有植物质粒的双系病毒DNA的方法和组合物。在另外的其它实施方案中，提供了方法和组合物，用于在不引起疾病症状的情况下使用含有本发明的含有双重病毒DNA的载体在植物中复制和表达异源DNA序列或基因。

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