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1. 发明授权

US07081535B2 Hydroxy-amino acids 有权
标题翻译：羟基 - 氨基酸
公开(公告)号：US07081535B2
公开(公告)日：2006-07-25
申请号：US11014423
申请日：2004-12-16
申请人： J. David Rozzell, Jr. , Spiros Kambourakis
发明人： J. David Rozzell, Jr. , Spiros Kambourakis
IPC分类号： C07D211/70 , C07D211/82 , C07D213/55 , C07D333/22 , C07C229/00
CPC分类号： C07C227/04 , C12N9/0006 , C12P13/04 , C07C229/22 , C07C229/34
摘要： Hydroxy-amino acids are provided and are prepared by contacting a substituted β-ketodiester having a ketone group and two ester functional groups with a ketoreductase under conditions permitting the reduction of the keytone group to an alcohol. Only one of the ester functional groups is regioselectively hydrolyzed to the corresponding carboxylic acid, whereby a non-hydrolyzed ester functional group remains. The carboxylic acid is converted to an amine to produce a hydroxy-amino acid.
摘要翻译：提供羟基 - 氨基酸，并且通过在允许将酮基团还原成醇的条件下使具有酮基团的取代的β-酮糖酯与酮还原酶与酮还原酶接触来制备。只有一个酯官能团被区域选择性地水解成相应的羧酸，由此残留非水解的酯官能团。将羧酸转化成胺以产生羟基 - 氨基酸。

2. 发明授权

US06833471B2 Methods for producing hydroxy amino acids and derivatives thereof 失效
标题翻译：羟基氨基酸及其衍生物的制备方法
公开(公告)号：US06833471B2
公开(公告)日：2004-12-21
申请号：US10237831
申请日：2002-09-09
申请人： J. David Rozzell, Jr. , Spiros Kambourakis
发明人： J. David Rozzell, Jr. , Spiros Kambourakis
IPC分类号： C07C22900
CPC分类号： C07C227/04 , C12N9/0006 , C12P13/04 , C07C229/22 , C07C229/34
摘要： A methods for producing a hydroxy-amino acid or derivative thereof, such as statine, phenylstatine or isostatine, is provided. A substituted &bgr;-ketodiester having a ketone group and two ester functional groups is contacted with a ketoreductase under conditions permitting the reduction of the ketone group to an alcohol. Only one of the ester functional groups is regioselectively hydrolyzed to the corresponding carboxylic acid, whereby a non-hydrolyzed ester functional group remains. Either the carboxylic acid or the non-hydrolyzed ester functional group is converted to an amine or a derivative thereof to produce a hydroxy-amino acid or derivative thereof.
摘要翻译：提供了用于制备羟基 - 氨基酸或其衍生物的方法，例如他汀类，苯基稳定剂或异ost ine啶。具有酮基和2个酯官能团的取代的β-酮糖酵母在酮还原成醇的条件下与酮还原酶接触。只有一个酯官能团被区域选择性地水解成相应的羧酸，由此残留非水解的酯官能团。将羧酸或未水解的酯官能团转化为胺或其衍生物以产生羟基 - 氨基酸或其衍生物。

3. 发明授权

US06818752B2 Synthetic genes for enhanced expression 失效
标题翻译：用于增强表达的合成基因
公开(公告)号：US06818752B2
公开(公告)日：2004-11-16
申请号：US09734237
申请日：2000-12-08
申请人： J. David Rozzell, Jr. , Peter Bui , Ling Hua
发明人： J. David Rozzell, Jr. , Peter Bui , Ling Hua
IPC分类号： C07H2104
CPC分类号： C07K14/21 , C12N15/1089 , C12N15/67
摘要： A synthetic nucleic acid sequence is disclosed, comprising a non-naturally occurring polymer of nucleic acids, having a biological function encoded by the sequence and known from a starting nucleic acid sequence, and having a difference in sequence of at least about 5% between the nucleic acids of the synthetic sequence and the starting sequence. The difference between the nucleic acid sequences results in a different free energy of folding for the synthetic sequence as compared to the starting sequence, such that the synthetic sequence would be expressed better in a selected heterologous host cell than the starting sequence would be if expressed in the same heterologous host cell.
摘要翻译：公开了一种合成核酸序列，其包含非天然存在的核酸聚合物，其具有由该序列编码并且从起始核酸序列已知的生物学功能，并且具有至少约5％之间的序列差异合成序列的核酸和起始序列。与起始序列相比，核酸序列之间的差异导致合成序列的折叠自由能不同，使得合成序列在选择的异源宿主细胞中比起始序列更好地表达，如果在相同的异源宿主细胞。

4. 发明授权

US5942644A Precursors for the production of chiral vicinal aminoalcohols 失效
标题翻译：用于生产手性连位氨基醇的前体
公开(公告)号：US5942644A
公开(公告)日：1999-08-24
申请号：US929474
申请日：1997-09-15
申请人： J. David Rozzell, Jr.
发明人： J. David Rozzell, Jr.
IPC分类号： C12P13/00 , C12P41/00 , C07C233/05 , C07C239/08
CPC分类号： C12P13/001 , C12P13/02 , C12P41/002
摘要： The disclosure describes new compositions of matter useful for the preparation of optically-active vicinal aminoalcohols. The compositions are chiral .beta.-hydroxycarboxamides, .beta.-hydroxyhydraxides, and .beta.-hydroxyhydroxamic acids.
摘要翻译：本公开描述了用于制备光学活性连位氨基醇的物质的新组合物。组合物是手性β-羟基甲酰胺，β-羟基氢氧化物和β-羟基异羟肟酸。

5. 发明授权

US5916786A Method for the production of chiral 1,3-aminoalcohols 失效
标题翻译：手性1,3-氨基醇的制备方法
公开(公告)号：US5916786A
公开(公告)日：1999-06-29
申请号：US994163
申请日：1997-12-19
申请人： J. David Rozzell, Jr.
发明人： J. David Rozzell, Jr.
IPC分类号： C12P13/00 , C12P7/02
CPC分类号： C12P13/001
摘要： The disclosure describes a method for the preparation of chiral 1,3-aminoalcohols in high optical purity. The method combines the stereoselective oxidation of a 1,4-diol to a gamma-lactone using an alcohol dehydrogenase, the conversion of the gamma-lactone to the corresponding 4-hydroxyamide, 4-hydroxyhydroxamic acid, or 4-hydroxyhydrazide, and stereospecific rearrangement of the 4-hydroxyamide, 4-hydroxyhydroxamic acid, or 4-hydroxyhydrazide to the corresponding chiral 1,3-aminoalcohol.
摘要翻译：本公开描述了制备高光学纯度的手性1,3-氨基醇的方法。该方法将使用醇脱氢酶的1,4-二醇的立体选择性氧化与γ-内酯相结合，将γ-内酯转化为相应的4-羟基酰胺，4-羟基异羟肟酸或4-羟基酰肼，并将立体特异性重排的4-羟基酰胺，4-羟基羟肟酸或4-羟基酰肼与相应的手性1,3-氨基醇反应。

6. 发明授权

US07642073B2 Method for reductive amination of a ketone using a mutated enzyme 有权
标题翻译：使用突变酶还原胺化酮的方法
公开(公告)号：US07642073B2
公开(公告)日：2010-01-05
申请号：US11786054
申请日：2007-04-09
申请人： J. David Rozzell, Jr.
发明人： J. David Rozzell, Jr.
IPC分类号： C12P13/00
CPC分类号： C12Y104/01009 , C12N9/0006 , C12N9/0016 , C12N9/0018 , C12P13/04
摘要： Methods for chemically transforming compounds using a mutated enzyme are provided, and more particularly a method for the production of an amine from a target ketone. The methods comprise creating a mutated enzyme that catalyzes the reductive amination of the target ketone under conditions sufficient to permit the formation of the desired amine to thereby produce the amine.
摘要翻译：提供了使用突变酶化学转化化合物的方法，更具体地，涉及一种从目标酮制备胺的方法。所述方法包括产生在足以允许形成所需胺以产生胺的条件下催化目标酮的还原胺化的突变酶。

7. 发明授权

US07301031B2 Methods for producing hydroxy amino acids, esters, and derivatives thereof 有权
标题翻译：羟基氨基酸，酯及其衍生物的制备方法
公开(公告)号：US07301031B2
公开(公告)日：2007-11-27
申请号：US11014261
申请日：2004-12-16
申请人： J. David Rozzell, Jr. , Spiros Kambourakis
发明人： J. David Rozzell, Jr. , Spiros Kambourakis
IPC分类号： C07C229/00 , C07D211/70 , C07D211/82 , C07D213/55 , C07D333/22
CPC分类号： C07C227/04 , C07D239/42 , C07D263/20 , C07D401/12 , C12N9/0006 , C12P13/04 , C07C229/22 , C07C229/34
摘要： A method for producing a hydroxy-amino ester, or derivative thereof, is provided. A substituted β-ketodiester having a ketone group and two ester functional groups is contacted with a ketoreductase under conditions permitting the reduction of the ketone group to an alcohol. Only one of the ester functional groups is regioselectively hydrolyzed to the corresponding carboxylic acid, whereby a non-hydrolyzed ester functional group remains. The carboxylic acid is converted to an amine or a derivative thereof to produce a hydroxy-amino ester or derivative thereof. A number of novel hydroxy-amino esters are prepared by the method.
摘要翻译：提供了制备羟基 - 氨基酯或其衍生物的方法。具有酮基和2个酯官能团的取代的β-酮糖酵母在酮还原成醇的条件下与酮还原酶接触。只有一个酯官能团被区域选择性地水解成相应的羧酸，由此残留非水解的酯官能团。将羧酸转化为胺或其衍生物以产生羟基 - 氨基酯或其衍生物。通过该方法制备了许多新的羟基 - 氨基酯。

8. 发明授权

US06207862B1 Precursors for the production of chiral 1,3-aminoalcohols 失效
标题翻译：用于生产手性1,3-氨基醇的前体
公开(公告)号：US06207862B1
公开(公告)日：2001-03-27
申请号：US09019390
申请日：1998-02-05
申请人： J. David Rozzell, Jr.
发明人： J. David Rozzell, Jr.
IPC分类号： C07C24100
CPC分类号： C12P13/001 , C12P13/02
摘要： The disclosure describes novel precursors for the preparation of chiral 1,3-aminoalcohols. The precursors are chiral 4-hydroxycarboxamides, 4-hydroxyhydroxamic acids, or 4-hydroxyhydrazides produced from chiral gamma-lactones, which in turn are derived from 1,4-diols by stereoselective oxidation. The chiral 4-hydroxycarboxamides, 4-hydroxyhydroxamic acids, or 4-hydroxyhydrazides are converted into chiral 1,3-aminoalcohols by stereospecific rearrangement.
摘要翻译：本公开描述了用于制备手性1,3-氨基醇的新型前体。前体是由手性γ-内酯制备的手性4-羟基甲酰胺，4-羟基异羟肟酸或4-羟基酰肼，其又通过立体选择性氧化衍生自1,4-二醇。手性4-羟基羧酰胺，4-羟基异羟肟酸或4-羟基酰肼通过立体特异性重排转化为手性1,3-氨基醇。

9. 发明授权

US5885767A Methods and compositions for quantitating L-homocysteine and/or l-methionine in a solution 失效
标题翻译：在溶液中定量L-高半胱氨酸和/或1-甲硫氨酸的方法和组合物
公开(公告)号：US5885767A
公开(公告)日：1999-03-23
申请号：US83459
申请日：1998-05-22
申请人： J. David Rozzell, Jr.
发明人： J. David Rozzell, Jr.
IPC分类号： C12Q1/25 , C12Q1/48 , C12Q1/527 , G01N33/68 , C12Q1/00 , C12Q1/37 , C12Q1/54
CPC分类号： C12Q1/25 , C12Q1/48 , C12Q1/527 , G01N33/6815
摘要： A method for quantitating L-homocysteine and/or L-methionine in a solution involves contacting a solution containing L-homocysteine and/or L-methionine with a reagent comprising methionine gamma-lyase and a cofactor capable of forming a Schiff base with the L-methionine and/or L-homocysteine for a time sufficient to catalyze the conversion of L-homocysteine and/or L-methionine to 2-ketobutyrate. The amount of 2-ketobutyrate formed is determined, and the amount of L-homocysteine and/or L-methionine present in the original solution can be determined based on the amount of 2-ketobutyrate formed. A composition for measuring the amount of L-homocysteine and/or L-methionine in a solution comprises methionine gamma-lyase, a cofactor capable of forming a Schiff base with the L-methionine and/or L-homocysteine and at least one 2-ketobutyrate detecting agent, but is substantially free of L-methionine, L-homocysteine, 2-ketobutyrate, pyruvate and mercury.
摘要翻译：在溶液中定量L-高半胱氨酸和/或L-甲硫氨酸的方法包括将含有L-高半胱氨酸和/或L-甲硫氨酸的溶液与含有甲硫氨酸γ-裂合酶的试剂和能够与L形成席夫碱的辅因子接触 - 甲硫氨酸和/或L-高半胱氨酸的时间足以催化L-高半胱氨酸和/或L-甲硫氨酸向2-酮丁酸转化。确定形成的2-酮基丁酸酯的量，并且可以基于形成的2-酮基丁酸酯的量来确定原始溶液中存在的L-高半胱氨酸和/或L-甲硫氨酸的量。用于测量溶液中L-高半胱氨酸和/或L-甲硫氨酸的量的组合物包含甲硫氨酸γ-裂解酶，能够与L-甲硫氨酸和/或L-高半胱氨酸形成希夫碱的辅因子和至少一种2- 酮基丁酸酯检测剂，但基本上不含L-甲硫氨酸，L-高半胱氨酸，2-酮丁酸酯，丙酮酸和汞。

10. 发明授权

US07202070B2 Method for reductive amination of a ketone using a mutated enzyme 有权
标题翻译：使用突变酶还原胺化酮的方法
公开(公告)号：US07202070B2
公开(公告)日：2007-04-10
申请号：US10039952
申请日：2001-10-24
申请人： J. David Rozzell, Jr.
发明人： J. David Rozzell, Jr.
IPC分类号： C12N9/06
CPC分类号： C12Y104/01009 , C12N9/0006 , C12N9/0016 , C12N9/0018 , C12P13/04
摘要： Methods for chemically transforming compounds using a mutated enzyme are provided, and more particularly a method for the production of an amino acid from a target 2-ketoacid, the production of an amine from a target ketone and the production of an alcohol from a target ketone. The methods comprise creating a mutated enzyme that catalyzes the reductive amination or transamination of the target 2-ketoacid or ketone or the reduction of the ketone and providing the mutated enzyme in a reaction mixture comprising the target 2-ketoacid or ketone under conditions sufficient to permit the formation of the desired amino acid, amine or alcohol to thereby produce the amino acid, amine or alcohol.
摘要翻译：提供了使用突变酶对化合物进行化学转化的方法，更具体地，涉及从靶酮生产氨基酸的方法，从目标酮生产胺和从目标酮生产醇。所述方法包括产生催化目标2-酮酸或酮的还原胺化或转氨酶或还原酮并在包含目标2-酮酸或酮的反应混合物中提供突变酶的突变酶，条件足以允许形成所需的氨基酸，胺或醇，从而产生氨基酸，胺或醇。

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