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1. 发明授权

US4742001A Method of terminating isocitrate dehydrogenase reaction in an analytical system 失效
标题翻译：在分析系统中终止异柠檬酸脱氢酶反应的方法
公开(公告)号：US4742001A
公开(公告)日：1988-05-03
申请号：US856440
申请日：1986-04-22
申请人： Yoji Marui , Takashi Nakano , Chozo Hayashi , Tuyosi Fujita , Isamu Takagahara
发明人： Yoji Marui , Takashi Nakano , Chozo Hayashi , Tuyosi Fujita , Isamu Takagahara
IPC分类号： C12Q1/28 , C12N9/99 , C12Q1/32 , C12Q1/34 , C12Q1/58
CPC分类号： C12Q1/32 , C12Q1/34 , C12Q1/58
摘要： Urea, creatinine, creatine, triglycerides, or the like in a specimen can be accurately determined by terminating an isocitrate dehydrogenase reaction by addition of ATP and/or a chelating agent in a system wherein NAD.sup.+ formed from NADH is reproduced into NADH in the conjoint presence of an isocitrate, metallic ions such as magnesium or manganese ions, and isocitrate dehydrogenase in assaying a substance by means of a reaction of NADH to NAD.sup.+.
摘要翻译：样品中的尿素，肌酸酐，肌酸，甘油三酯等可以通过在其中由NADH形成的NAD +在NADH中共同存在的系统中加入ATP和/或螯合剂来终止异柠檬酸脱氢酶反应来精确测定的异柠檬酸盐，金属离子如镁或锰离子，以及异柠檬酸脱氢酶，通过NADH与NAD +的反应来测定物质。

2. 发明授权

US5436133A Enzyme assay of biochemical substances 失效
标题翻译：生化物质酶检测
公开(公告)号：US5436133A
公开(公告)日：1995-07-25
申请号：US865715
申请日：1992-04-08
申请人： Tuyosi Fujita , Isamu Takagahara
发明人： Tuyosi Fujita , Isamu Takagahara
IPC分类号： C12Q1/28 , C12Q1/32 , G01N33/50 , G01N33/52
CPC分类号： C12Q1/32 , C12Q1/28 , Y10S435/962 , Y10S436/825 , Y10T436/10
摘要： This invention relates to an enzyme assay for quantitative analysis of biochemical substances using NAD(P)-NAD(P)H system and utilizing hydrogen peroxide, in which isocitric acid, metal ions and isocitrate dehydrogenase are previously added to a test sample, thereby consuming the endogenous substances that interfere the analysis, and at the same time regenerating NAD(P)H reduced.The isocitrate dehydrogenase is then inactivated by addition of a chelating agent, an enzyme or substrate that generates hydrogen peroxide as reaction product is added simultaneously or thereafter, and the amount of hydrogen peroxide thus formed is measured.Biochemical substances that generate hydrogen peroxide as reaction product can be correctly analyzed by this method with no adverse effect of endogenous substances because these interfering substances have been removed prior to measurement.
摘要翻译：本发明涉及使用NAD（P）-NAD（P）H系统并利用过氧化氢定量分析生化物质的酶测定法，其中异丙酸，金属离子和异柠檬酸脱氢酶预先加入到测试样品中，从而消耗干扰分析的内源性物质，同时还原NAD（P）H。然后通过加入螯合剂，产生过氧化氢的酶或底物，同时或之后加入异柠檬酸脱氢酶，并测量这样形成的过氧化氢的量。生成过氧化氢作为反应产物的生化物质可以通过这种方法进行正确分析，无内生物质的不利影响，因为这些干扰物质在测量前已被去除。

3. 发明授权

US4107014A Isoelectric point markers for gel isoelectric separation 失效
标题翻译：用于凝胶等电分离的等电点标记
公开(公告)号：US4107014A
公开(公告)日：1978-08-15
申请号：US754976
申请日：1976-12-28
申请人： Yasuo Suzuki , Isamu Takagahara , Tsuyoshi Fujita , Katsumi Fujii , Takekazu Horio
发明人： Yasuo Suzuki , Isamu Takagahara , Tsuyoshi Fujita , Katsumi Fujii , Takekazu Horio
IPC分类号： G01N27/26 , G01N27/447 , G01N30/04
CPC分类号： G01N27/44795 , G01N27/44726 , Y10T436/25375
摘要： Isoelectric point markers for a gel isoelectric separation are prepared to comprise at least two colored proteins of which the isoelectric point pHs are known and wherein the difference between the highest value and the lowest value among the said pHs is not less than 0.5. The markers are applied to analyze proteins in a gel isoelectric separation method. The method employing the said markers makes it easy to analyze proteins and shows an excellent performance.
摘要翻译：制备用于凝胶等电点分离的等电点标记物，其包含其中等电点pH值已知的至少两种着色蛋白质，其中所述pH值中最高值和最低值之间的差不小于0.5。应用标记物以凝胶等电分离方法分析蛋白质。使用所述标记的方法使得易于分析蛋白质并显示出优异的性能。

4. 发明授权

US5108905A Method of assaying magnesium in human body fluid 失效
标题翻译：人体体液中镁的测定方法
公开(公告)号：US5108905A
公开(公告)日：1992-04-28
申请号：US690420
申请日：1991-04-24
申请人： Yoshiko Sunahara , Shigeru Takata , Isamu Takagahara
发明人： Yoshiko Sunahara , Shigeru Takata , Isamu Takagahara
IPC分类号： C12Q1/32 , G01N33/84
CPC分类号： G01N33/84 , C12Q1/32 , Y10S435/81
摘要： Disclosed is a method of assaying the content of Mg ions in a human body fluid such as serum, urine or saliva by the use of a reactant solution containing isocitrate dehydrogenase, NADP.sup.+, isocitrate and an excess amount of a chelating agent. Almost all Mg ions as existing in a human body fluid sample to be examined are bonded with the chelating agent, and the remaining Mg ions react with NADP.sup.+ to form NADPH. Increase of the thus formed NADPH is measured to obtain a standard curve of a straight line, and the content of Mg ions in the same is determined on the basis of the standard curve. If such an excess amount of a Mg-chelating agent is not added to the reactant solution, the intended standard could not be in the form of a straight line but is in the form of a tangent curve. Using the tangent curve, the content of Mg ions in the human body fluid sample cannot be assayed accurately.

5. 发明授权

US4994374A Diagnostic method of cirrhosis and hepatic cancer 失效
标题翻译：肝硬化和肝癌诊断方法
公开(公告)号：US4994374A
公开(公告)日：1991-02-19
申请号：US362414
申请日：1989-05-01
申请人： Atsushi Nishikawa , Naoyuki Taniguchi , Isamu Takagahara
发明人： Atsushi Nishikawa , Naoyuki Taniguchi , Isamu Takagahara
IPC分类号： G01N33/50 , A61K47/48 , C12Q1/48 , G01N33/573 , G01N33/574
CPC分类号： A61K47/48238 , C12Q1/48 , G01N33/57438 , G01N33/57469
摘要： This invention relates to a method of diagnosing cancerous diseases, which comprises measuring the amount of UDP-N-acetylglucosamine:glycoprotein N-acetylglucosaminyl-transferase in body fluid and evaluating the increase in its amount for the diagnosis of hepatic diseases.AFP, CEA and .gamma.-glutamyltranspeptidase have hitherto been used as tumor markers for the diagnosis of hepatic cancer. But these conventional tumor markers show a positivity rate of about 60%, making early diagnosis almost impossible.The method of this invention employs UDP-N-acetylglucosamine:glycoprotein N-acetylglucosaminyltransferase as tumor marker, whereby early diagnosis of hepatic cancer can be made almost completely.
摘要翻译： PCT No.PCT / JP88 / 00898 Sec。 371日期：1989年5月1日 102（e）日期1989年5月1日PCT提交1988年9月6日PCT公布。出版物WO89 / 02474 本发明涉及一种诊断癌性疾病的方法，其包括测量体液中UDP-N-乙酰葡糖胺：糖蛋白N-乙酰氨基葡糖转移酶的量，并评估其用于诊断的量的增加肝病。 AFP，CEA和γ-谷氨酰转肽酶迄今已被用作肝癌诊断的肿瘤标志物。但这些常规肿瘤标志物的阳性率约为60％，使得早期诊断几乎不可能。本发明的方法采用UDP-N-乙酰氨基葡萄糖：糖蛋白N-乙酰葡糖胺基转移酶作为肿瘤标志物，从而能够几乎完全地进行肝癌的早期诊断。

6. 发明申请

US20050153377A1 Method for determining CRP using phosphorylcholine 审中-公开
标题翻译：使用磷酸胆碱测定CRP的方法
公开(公告)号：US20050153377A1
公开(公告)日：2005-07-14
申请号：US10866247
申请日：2004-06-14
申请人： Hiroshi Tamura , Y.C. Lee , Isamu Takagahara , Yuhsi Matuo , Keiko Saito , Kichitaro Kawaguchi
发明人： Hiroshi Tamura , Y.C. Lee , Isamu Takagahara , Yuhsi Matuo , Keiko Saito , Kichitaro Kawaguchi
IPC分类号： G01N33/53 , G01N33/533 , G01N33/58 , G01N33/68 , G01N33/537 , G01N33/543
CPC分类号： G01N33/582 , G01N33/68 , G01N2333/4737 , G01N2458/40
摘要： The present invention provides a method for determining the concentration of C-reactive protein in a sample using labeled phosphorylcholine.
摘要翻译：本发明提供了使用标记的磷酸胆碱来测定样品中C-反应蛋白的浓度的方法。

7. 发明授权

US4356072A Isoelectric point marker 失效
标题翻译：等电点标记
公开(公告)号：US4356072A
公开(公告)日：1982-10-26
申请号：US217917
申请日：1980-12-18
申请人： Hiraku Saito , Isamu Takagahara , Yasuo Suzuki , Tuyosi Fujita , Katsumi Fujii , Takekazu Horio
发明人： Hiraku Saito , Isamu Takagahara , Yasuo Suzuki , Tuyosi Fujita , Katsumi Fujii , Takekazu Horio
IPC分类号： A61B5/00 , B01D57/02 , C07K1/113 , C07K1/28 , C07K14/41 , C07K14/415 , G01N27/26 , G01N27/447 , G01N31/22
CPC分类号： G01N27/44795 , B01D57/02 , G01N27/44747
摘要： A group of compounds having the following general formula can be obtained by reacting chromoprotein and organic acid: ##STR1## wherein X is a chromophore, P is a protein, NH.sub.2 is mainly .epsilon.-amino group of lysine, n is a positive integer of 1 to 18, and m is a positive integer in the relation of m.ltoreq.n. This compound group has one to eighteen organic acids bonded thereto, and shows various isoelectric points in accordance with the number of the organic acids, but the respective isoelectric points are maintained constant. If such compounds each having an individual isoelectric point, are used as isoelectric point markers, it is possible to recognize accurately the position of isoelectric point only by a visual operation.
摘要翻译：一组具有以下通式的化合物可以通过发色蛋白和有机酸反应获得：其中X是发色团，P是蛋白质，NH2主要是赖氨酸的ε-氨基，n是正整数1 18，m是m

8. 发明授权

US4258131A Method of fractional quantitative determination of isoenzyme of lactic dehydrogenase 失效
$Method of fractional quantitative determination of isoenzyme of lactic dehydrogenase$
标题翻译：乳酸脱氢酶同工酶分数定量方法
公开(公告)号：US4258131A
公开(公告)日：1981-03-24
申请号：US54416
申请日：1979-07-03
申请人： Isamu Takagahara , Juniti Yamauti , Setsuko Yoshimura , Katsumi Fujii , Takekazu Horio
发明人： Isamu Takagahara , Juniti Yamauti , Setsuko Yoshimura , Katsumi Fujii , Takekazu Horio
IPC分类号： C12N9/99 , C12Q1/26 , C12Q1/32
CPC分类号： C12Q1/32
摘要： 1,6-Dihydro nicotinamide adenine dinucleotide inhibits both H-type lactic dehydrogenase and M-type lactic dehydrogenase which are isoenzymes of lactic dehydrogenase, but the degree of inhibition thereof against H-type considerably differs from that against M-type. A ratio of H-type lactic dehydrogenase to M-type lactic dehydrogenase in serum can be measured by utilizing the difference of inhibition degree. Therefore we can diagnose the organ with trouble.
摘要翻译： 1,6-二氢烟酰胺腺嘌呤二核苷酸抑制作为乳酸脱氢酶同工酶的H型乳酸脱氢酶和M型乳酸脱氢酶，但与H型相比，其抑制程度明显不同于M型。利用抑制程度的差异可以测定血清中H型乳酸脱氢酶与M型乳酸脱氢酶的比例。所以我们可以诊断器官有麻烦。

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