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1. 发明申请

US20110162112A1 INCREASING TIME-EFFICIENCY OF HIGH-THROUGHPUT TRANSFORMATION PROCESSES 审中-公开
标题翻译：提高高速转换过程的时效性
公开(公告)号：US20110162112A1
公开(公告)日：2011-06-30
申请号：US12976555
申请日：2010-12-22
申请人： Yinghong Li , Igor C. Oliveira
发明人： Yinghong Li , Igor C. Oliveira
IPC分类号： C12N15/82 , C12N15/87 , C12N15/89
CPC分类号： C12N15/8205
摘要： The methods provided relate to efficient methods for transforming isolated, immature maize embryos and for producing transgenic maize plantlets. The time required for the production of the transgenic plantlets and subsequent plants is significantly decreased compared to conventional methods. The methods also relate to decreasing the selection time of transgenic events and regenerating a transgenic maize plantlet from transgenic somatic embryos from the events in a plant cell culture vessel that allows for root formation and plantlet elongation in the same plant cell culture vessel.
摘要翻译：所提供的方法涉及用于转化分离的，未成熟的玉米胚胎和用于产生转基因玉米小植株的有效方法。与常规方法相比，生产转基因植物和随后植物所需的时间显着降低。所述方法还涉及减少转基因事件的选择时间并从植物细胞培养容器中的事件中转基因体细胞胚再生转基因玉米小植物，其允许相同植物细胞培养容器中的根形成和小植株伸长。

2. 发明授权

US07928287B2 Methods for large scale functional evaluation of nucleotide sequences in plants 有权
标题翻译：植物核苷酸序列大规模功能评估方法
公开(公告)号：US07928287B2
公开(公告)日：2011-04-19
申请号：US10367417
申请日：2003-02-13
申请人： Dwight T. Tomes , Deping Xu , Haiyin Wang , Xiaomu Niu , Igor C. Oliveira
发明人： Dwight T. Tomes , Deping Xu , Haiyin Wang , Xiaomu Niu , Igor C. Oliveira
IPC分类号： A01H1/00 , A01H1/02
CPC分类号： C12N15/1082
摘要： The present invention provides for rapid and large scale evaluation of expression of, or function of, nucleotide sequences in plants. The invention comprises three specific components which provide for fast and large scale evaluation of nucleotide sequences. The first component includes delivery in either a single event a library of different engineered vectors or a single engineered vector for a single target nucleotide sequence comprising sequences the function of which is desired to be known in plant cells. Surprisingly, applicants have discovered that, the introduction of multiple vectors to plant cells predominantly results in individual transgenic plants which contain only a single transformation event. The second feature of the invention involves a highly transformable, fast cycling and/or miniature size plant and the final step involves mass scale analysis of T0 plants for various phenotypes and plasmid rescue to identify the nucleotide sequence present in a particular phenotype.
摘要翻译：本发明提供对植物中核苷酸序列的表达或功能的快速和大规模评估。本发明包括提供快速和大规模评估核苷酸序列的三个特定组分。第一组分包括在单一事件中递送不同工程化载体的文库或单个靶核苷酸序列的单一工程载体，其包含其功能期望在植物细胞中已知的序列。令人惊讶的是，申请人已经发现，将多个载体引入植物细胞主要导致单个转基因植物，其仅包含单个转化事件。本发明的第二个特征涉及高度可变形，快速循环和/或微型尺寸的植物，并且最终步骤涉及用于各种表型和质粒拯救的T0植物的大规模分析，以鉴定特定表型中存在的核苷酸序列。

3. 发明申请

US20100178698A1 TISSUE CULTURE DEVICE 审中-公开
标题翻译：组织文化装置
公开(公告)号：US20100178698A1
公开(公告)日：2010-07-15
申请号：US12650010
申请日：2009-12-30
申请人： STEVEN M. BECKER , SAM ELLIS , YINGHONG LI , IGOR C. OLIVEIRA
发明人： STEVEN M. BECKER , SAM ELLIS , YINGHONG LI , IGOR C. OLIVEIRA
IPC分类号： C12N5/02 , C12M1/12 , C12N5/04 , C12N1/16 , C12N1/14 , C12N5/07
CPC分类号： C12M47/02 , C12M23/10 , C12M25/02
摘要： A tissue culture device includes a container having one or more upstanding walls extending upwardly from a floor. The floor of the container has a media having nutrients or growth substances therein. A plurality of plant tissues are within the container compartment and are placed upon a screen between the plant tissues and the media. The screen is tamped downwardly onto the media so that the plant tissues can get nutrients from the media, and so that waste products are transferred into the media. The screen is also removable through the open end of the container so as to remove all of the plurality of the plant tissues from the container at once. The plant tissue device can then be placed in another container having any cultured media as needed for the correct maintenance, propagation and development of plant tissue in culture.
摘要翻译：组织培养装置包括具有从地板向上延伸的一个或多个直立壁的容器。容器的底部具有其中具有营养物质或生长物质的介质。多个植物组织在容器隔室内并且放置在植物组织和培养基之间的筛网上。将屏幕向下夯实到介质上，使得植物组织可以从培养基中获得营养物质，并且将废物转移到培养基中。屏幕也可以通过容器的开口端移除，以便立即将所有多个植物组织从容器中移出。然后可以将植物组织装置放置在具有培养基中的任何培养基的另一容器中，以正确维持，繁殖和培养培养物中植物组织。

4. 发明授权

US08334426B1 Method for obtaining transformed maize plants 失效
标题翻译：获得转化玉米植物的方法
公开(公告)号：US08334426B1
公开(公告)日：2012-12-18
申请号：US12478008
申请日：2009-06-04
申请人： Yinghong Li , Jijun Zou , Igor C. Oliveira
发明人： Yinghong Li , Jijun Zou , Igor C. Oliveira
IPC分类号： C12N15/82
CPC分类号： C12N15/8205
摘要： Methods are provided for transforming isolated, immature maize embryos and for producing transgenic maize plants. The methods additionally comprise identifying or selecting transformed cells and regenerating such cells into transformed maize plants.
摘要翻译：提供了用于转化分离的，未成熟的玉米胚胎和用于产生转基因玉米植物的方法。所述方法另外包括鉴定或选择转化的细胞并将此类细胞再生成转化的玉米植物。

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