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1. 发明授权

US08511659B2 Holding jig, measurement device and holding device using the same 有权
标题翻译：保持夹具，测量装置和使用其的保持装置
公开(公告)号：US08511659B2
公开(公告)日：2013-08-20
申请号：US12722023
申请日：2010-03-11
申请人： Toshihiro Hirakawa , Satoshi Sakashita , Shinji Ishida , Hiroyuki Nagaoka
发明人： Toshihiro Hirakawa , Satoshi Sakashita , Shinji Ishida , Hiroyuki Nagaoka
IPC分类号： B23Q3/08 , B23Q3/00 , B25B1/20 , B25B1/06
CPC分类号： B25B1/18 , B25B1/20 , B25B1/241 , B25B1/2415 , B25B1/2473 , B25B5/08 , B25B5/087 , B25B5/147 , B25B5/163
摘要： A holding jig including, a tilt angle with respect to a plane of a holding surface of a holding target defined as a holding tilt angle: a first holding portion having a holding tilt face with the holding tilt angle; a second holding portion connected to the first holding portion so as to slide toward the holding tilt face along a plane substantially parallel to the holding surface; and an elastic holding portion provided at a position of the second holding portion facing the holding tilt face that is elastically deformed towards the holding surface by the holding tilt face while being abutted to the holding tilt face, when the second holding portion slides toward the holding tilt face, to press and hold the holding target.
摘要翻译：保持夹具，其包括相对于保持对象的保持面的平面的倾斜角，被定义为保持倾斜角：具有保持倾斜面的保持倾斜角的第一保持部; 第二保持部，其连接到所述第一保持部，以沿着与所述保持面大致平行的平面朝向所述保持倾斜面滑动; 以及弹性保持部，其设置在所述第二保持部的位于所述保持倾斜面的位置，所述保持倾斜面在所述保持倾斜面抵靠所述保持倾斜面时朝向所述保持面弹性变形，倾斜面，按住保持目标。

2. 发明授权

US08852904B2 Protein complex having activity catalyzing asymmetric oxidation reaction and process for producing the same 有权
标题翻译：具有催化不对称氧化反应的活性的蛋白质复合物及其制备方法
公开(公告)号：US08852904B2
公开(公告)日：2014-10-07
申请号：US13321520
申请日：2010-05-20
申请人： Hiroyuki Nagaoka
发明人： Hiroyuki Nagaoka
IPC分类号： C12P7/02 , A61L33/00 , C12P7/26 , C12N11/04 , C12P41/00 , C12N9/96 , C12N9/02
CPC分类号： C12P7/02 , C12N9/0004 , C12N9/96 , C12N11/04 , C12P7/26 , C12P41/002 , Y02P20/52 , Y02P20/588
摘要： A process for producing a cross-linked crystallized protein complex, which comprises: a first step of concentrating a crude protein derived from an animal or plant; a second step of encapsulating the protein in a gel, to thereby allow the protein to undergo air oxidation, and then extracting a protein complex from the gel; a third step of allowing the extracted protein complex to undergo crystallization and precipitation; and a fourth step of cross-linking the precipitated protein complex. Alternatively, by use of a fifth step of drying (FD) the obtained crosslinked crystallized protein complex, to thereby form a powder. As a result, there is provided an enzyme which is stable at room temperature storage, and has an activity in catalyzing an asymmetric oxidation reaction. That is, there is provided a useful material which enables an efficient enzyme-mimetic reaction under a mild condition.
摘要翻译：一种生产交联结晶蛋白复合物的方法，其包括：浓缩源自动物或植物的粗蛋白质的第一步骤; 将蛋白质包封在凝胶中的第二步骤，从而使蛋白质经历空气氧化，然后从凝胶中提取蛋白质复合物; 第三步，使提取的蛋白质复合物进行结晶析出; 以及交联沉淀的蛋白质复合物的第四步骤。或者，通过使用所获得的交联结晶蛋白复合物的干燥（FD）的第五步骤，从而形成粉末。结果，提供了一种在室温下稳定的酶，并且具有催化不对称氧化反应的活性。也就是说，提供了在温和条件下能够进行有效的酶模拟反应的有用材料。

3. 发明授权

US06218581B1 Process of producing optically active alcohol 有权
标题翻译：制备光学活性醇的方法
公开(公告)号：US06218581B1
公开(公告)日：2001-04-17
申请号：US09367137
申请日：1999-08-06
申请人： Hiroyuki Nagaoka
发明人： Hiroyuki Nagaoka
IPC分类号： C07C2909
CPC分类号： C12P41/00 , C12P7/02 , C12P41/002 , C12P41/004
摘要： The present invention relates to a process of producing optically active alcohol having high optical purity by performing an enzyme conversion reaction on a raw material serving as the substrate, examples of which include racemic alcohol, ketone and the acylated form of a racemic alcohol, followed by hydrolysis as necessary, using as an optical separation catalyst a immobilized water-soluble protein extracted from inexpensive and safe grains or beans in order to solve the problems of the reaction process consisting of low yield due to the occurrence of side reactions, detrimental effects on the ecosystem and their accompanying cost burden, the need for aseptic procedures and the extended amount of time of subculturing, all of which are problems associated with production processes of optically active alcohols of the prior art that use microorganisms, microorganism enzymes, animal tissue enzymes and cultured plant cells.
摘要翻译：本发明涉及通过对作为底物的原料进行酶转化反应来制造光学纯度高的光学活性醇的方法，其实例包括外消旋醇，酮和酰化形式的外消旋醇，然后是根据需要进行水解，使用从廉价且安全的谷物或豆提取的固定化的水溶性蛋白质作为光学分离催化剂，以解决由于副反应的产生而产生的低产率的反应过程的问题，对副产物的不利影响生态系统及其伴随的成本负担，无菌程序的需要和延长的传代时间，所有这些都是与使用微生物，微生物酶，动物组织酶和培养的现有技术的光学活性醇的生产方法相关的问题植物细胞。

4. 发明申请

US20120064588A1 PROTEIN COMPLEX HAVING ACTIVITY CATALYZING ASYMMETRIC OXIDATION REACTION AND PROCESS FOR PRODUCING THE SAME 有权
标题翻译：具有活性催化不对称氧化反应的蛋白复合物及其生产方法
公开(公告)号：US20120064588A1
公开(公告)日：2012-03-15
申请号：US13321520
申请日：2010-05-20
申请人： Hiroyuki Nagaoka
发明人： Hiroyuki Nagaoka
IPC分类号： C12P7/02 , C12N9/02
CPC分类号： C12P7/02 , C12N9/0004 , C12N9/96 , C12N11/04 , C12P7/26 , C12P41/002 , Y02P20/52 , Y02P20/588
摘要： A process for producing a cross-linked crystallized protein complex, which comprises: a first step of concentrating a crude protein derived from an animal or plant; a second step of encapsulating the protein in a gel, to thereby allow the protein to undergo air oxidation, and then extracting a protein complex from the gel; a third step of allowing the extracted protein complex to undergo crystallization and precipitation; and a fourth step of cross-linking the precipitated protein complex. Alternatively, by use of a fifth step of drying (FD) the obtained crosslinked crystallized protein complex, to thereby form a powder. As a result, there is provided an enzyme which is stable at room temperature storage, and has an activity in catalyzing an asymmetric oxidation reaction. That is, there is provided a useful material which enables an efficient enzyme-mimetic reaction under a mild condition.
摘要翻译：一种生产交联结晶蛋白复合物的方法，其包括：浓缩源自动物或植物的粗蛋白质的第一步骤; 将蛋白质包封在凝胶中的第二步骤，从而使蛋白质经历空气氧化，然后从凝胶中提取蛋白质复合物; 第三步，使提取的蛋白质复合物进行结晶析出; 以及交联沉淀的蛋白质复合物的第四步骤。或者，通过使用所获得的交联结晶蛋白复合物的干燥（FD）的第五步骤，从而形成粉末。结果，提供了一种在室温下稳定的酶，并且具有催化不对称氧化反应的活性。也就是说，提供了在温和条件下能够进行有效的酶模拟反应的有用材料。

5. 发明申请

US20100244345A1 HOLDING JIG, MEASUREMENT DEVICE AND HOLDING DEVICE USING THE SAME 有权
标题翻译：保持夹具，测量装置和使用其的保持装置
公开(公告)号：US20100244345A1
公开(公告)日：2010-09-30
申请号：US12722023
申请日：2010-03-11
申请人： Toshihiro HIRAKAWA , Satoshi Sakashita , Shinji Ishida , Hiroyuki Nagaoka
发明人： Toshihiro HIRAKAWA , Satoshi Sakashita , Shinji Ishida , Hiroyuki Nagaoka
IPC分类号： B25B11/00 , G01L7/00
CPC分类号： B25B1/18 , B25B1/20 , B25B1/241 , B25B1/2415 , B25B1/2473 , B25B5/08 , B25B5/087 , B25B5/147 , B25B5/163
摘要： A holding jig includes, in a case where a tilt angle with respect to a plane as a holding surface of a holding target is defined as a holding tilt angle: a first holding portion having a holding tilt face with the holding tilt angle; a second holding portion connected to the first holding portion so as to slide toward the holding tilt face along a plane substantially parallel to the holding surface; and an elastic holding portion provided at a position of the second holding portion facing the holding tilt face and elastically deformed toward the holding surface by the holding tilt face while abutting on the holding tilt face, when the second holding portion slides toward the holding tilt face, to press and hold the holding target.
摘要翻译：保持夹具包括在相对于作为保持目标的保持表面的平面的倾斜角被定义为保持倾斜角度的情况下：具有保持倾斜面的第一保持部分具有保持倾斜角; 第二保持部，其连接到所述第一保持部，以沿着与所述保持面大致平行的平面朝向所述保持倾斜面滑动; 以及弹性保持部，其设置在所述第二保持部的与所述保持倾斜面对置的位置，并且当所述第二保持部朝向所述保持倾斜面滑动时，由所述保持倾斜面朝向所述保持面弹性变形，按住持有目标。

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