专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 再颁专利

USRE44522E1 Concentration measuring method, concentration test instrument, and concentration measuring apparatus 有权
公开(公告)号：USRE44522E1
公开(公告)日：2013-10-08
申请号：US12822869
申请日：2010-06-24
申请人： Kenji Nagakawa , Hideaki Yamaoka
发明人： Kenji Nagakawa , Hideaki Yamaoka
IPC分类号： G01N27/416
摘要： The present invention relates to technology for constructing a reaction system including a test target, an oxidation-reduction enzyme, and an electron mediator, and measuring the concentration of the test target by an electrochemical process. A Ru compound is used as the electron mediator. The present invention provides a concentration test instrument including a substrate, first and second electrodes formed on the substrate, and a reagent layer formed as a solid. The reagent layer contains an oxidation-reduction enzyme and a Ru compound, and is constituted so as to dissolve and construct a liquid phase reaction system when a sample liquid is supplied.

2. 发明申请

US20110076707A1 MUTANT GLUCOSE DEHYDROGENASE 有权
公开(公告)号：US20110076707A1
公开(公告)日：2011-03-31
申请号：US12861734
申请日：2010-08-23
申请人： Hideaki Yamaoka
发明人： Hideaki Yamaoka
IPC分类号： C12Q1/54 , C07H21/04 , C12N1/00
CPC分类号： C12N9/0006 , C12Q1/32
摘要： Substrate specificity for glucose of a glucose dehydrogenase having the amino acid sequence of SEQ ID NO: 13 is improved by substituting another amino acid residue for the amino acid residue at position 472 and/or 475.
摘要翻译：通过用另一个氨基酸残基取代位置472和/或475处的氨基酸残基来提高具有SEQ ID NO：13氨基酸序列的葡萄糖脱氢酶对葡萄糖的葡萄糖的底物特异性。

3. 发明授权

US07390391B2 Concentration measuring method, concentration test instrument, and concentration measuring apparatus 有权
标题翻译：浓度测定法，浓度检测仪，浓度测定仪
公开(公告)号：US07390391B2
公开(公告)日：2008-06-24
申请号：US10489488
申请日：2002-08-30
申请人： Kenji Nagakawa , Hideaki Yamaoka
发明人： Kenji Nagakawa , Hideaki Yamaoka
IPC分类号： G01N27/416
CPC分类号： C12Q1/004
摘要： The present invention relates to technology for constructing a reaction system including a test target, an oxidation-reduction enzyme, and an electron mediator, and measuring the concentration of the test target by an electrochemical process. A Ru compound is used as the electron mediator. The present invention provides a concentration test instrument including a substrate, first and second electrodes formed on the substrate, and a reagent layer formed as a solid. The reagent layer contains an oxidation-reduction enzyme and a Ru compound, and is constituted so as to dissolve and construct a liquid phase reaction system when a sample liquid is supplied.
摘要翻译：本发明涉及用于构建包括测试对象，氧化还原酶和电子介体的反应系统的技术，并且通过电化学过程测量测试目标的浓度。使用Ru化合物作为电子介体。本发明提供了一种浓度测试仪器，其包括基板，形成在基板上的第一和第二电极以及形成为固体的试剂层。试剂层含有氧化还原酶和Ru化合物，构成为在供给样品液时溶解并构成液相反应体系。

4. 发明申请

US20060042361A1 Measuring instrument provided with solid composnent concentrating means 有权
标题翻译：测量仪器配有固体成分浓缩装置
公开(公告)号：US20060042361A1
公开(公告)日：2006-03-02
申请号：US10533602
申请日：2003-10-30
申请人： Hideaki Yamaoka , Koji Katsuki
发明人： Hideaki Yamaoka , Koji Katsuki
IPC分类号： G01N1/40
CPC分类号： C12Q1/006 , C12Q1/001 , C12Q1/005 , G01N27/3272
摘要： The present invention relates to a measuring instrument (1) comprising a channel (60) for moving a sample liquid (BL) containing a solid component (B1) and providing a liquid reaction field and first and second electrodes (31, 32) which are used to apply voltage to the liquid reaction field. The first electrode (31) has an electron transfer interface (31a) for transferring electrons between it and the liquid reaction field when voltage is applied to the liquid reaction field via the first and second electrodes (31, 32). The measuring instrument (1) comprises concentrating means (51) for increasing the concentration of solid components at portions thereof which contact the electron transfer interface (31a) in the liquid reaction field. The concentrating means (51) preferably comprises a water-absorbing layer containing an absorbent polymer material.
摘要翻译：本发明涉及一种测量仪器（1），其包括用于移动含有固体组分（B 1）并提供液体反应场的样品液体（BL）的通道（60）和第一和第二电极（31,32），所述第一和第二电极用于向液体反应场施加电压。当电压通过第一和第二电极（31,32）施加到液体反应场时，第一电极（31）具有用于在其与液体反应场之间转移电子的电子转移界面（31a）。测量仪器（1）包括浓缩装置（51），用于增加在液体反应场中接触电子传递界面（31a）的部分处的固体组分的浓度。浓缩装置（51）优选包括含有吸收性聚合物材料的吸水层。

5. 发明授权

US08210349B2 Sensor storage container 有权
标题翻译：传感器存储容器
公开(公告)号：US08210349B2
公开(公告)日：2012-07-03
申请号：US10550287
申请日：2004-03-25
申请人： Hideaki Yamaoka
发明人： Hideaki Yamaoka
IPC分类号： A61B19/02 , B65D85/00 , B65D85/62
CPC分类号： G01N33/48778 , C12Q1/004
摘要： A sensor storage container is provided that allows sensors to be checked visually from the outside. A container is used for storing sensors including an oxidation-reduction enzyme that oxidizes or reduces an object to be analyzed, a mediator that mediates the transfer of electrons caused by oxidation or reduction, and a detection means that detects the oxidation or reduction reaction. The container is transparent or semi-transparent in whole or in part and thereby the sensors can be checked visually from the outside of the container. This container may be composed of a container body and a lid. The container body may be provided with a scale. Preferably, the sensors are those produced using a ruthenium metal complex as the mediator.
摘要翻译：提供了一种传感器存储容器，其允许从外部视觉检查传感器。容器用于存储氧化或还原被分析物的氧化还原酶的传感器，介导由氧化或还原引起的电子传递的介体以及检测氧化还原反应的检测装置。容器全部或部分透明或半透明，从而可以从容器的外部视觉检查传感器。该容器可以由容器主体和盖子组成。容器主体可以设置有秤。优选地，传感器是使用钌金属络合物作为介体产生的传感器。

6. 发明授权

US07803592B2 Mutant glucose dehydrogenase 有权
标题翻译：突变葡萄糖脱氢酶
公开(公告)号：US07803592B2
公开(公告)日：2010-09-28
申请号：US11587333
申请日：2005-04-22
申请人： Hideaki Yamaoka
发明人： Hideaki Yamaoka
IPC分类号： C12N9/04 , C12N1/20 , C12N15/00 , C12Q1/00 , C12Q1/68 , C12Q1/26 , C12P21/04 , C07H21/04
CPC分类号： C12N9/0006 , C12Q1/32
摘要： Substrate specificity for glucose of a glucose dehydrogenase having the amino acid sequence of SEQ ID NO: 13 is improved by substituting another amino acid residue for the amino acid residue at position 472 and/or 475.
摘要翻译：通过用另一个氨基酸残基取代位置472和/或475处的氨基酸残基来改善具有SEQ ID NO：13氨基酸序列的葡萄糖脱氢酶对葡萄糖的底物特异性。

7. 发明申请

US20090101499A1 Protein-Immobilized membrane, method for immobilization of protein, enzyme-immobilized electrode, and biosensor 审中-公开
标题翻译：蛋白质固定化膜，固定蛋白质的方法，固定酶的电极和生物传感器
公开(公告)号：US20090101499A1
公开(公告)日：2009-04-23
申请号：US11920782
申请日：2006-05-18
申请人： Koji Katsuki , Hideaki Yamaoka
发明人： Koji Katsuki , Hideaki Yamaoka
IPC分类号： G01N27/26 , C12N11/16
CPC分类号： G01N27/3272 , C07K14/705 , C07K14/80 , C12N9/0006 , C12N11/06 , C12N11/08 , C12Q1/006 , G01N2333/80
摘要： The present invention relates to a protein-immobilized membrane (14) including a cell membrane homologous layer (14A) and a protein (14B) immobilized to the cell membrane homologous layer (14A), where the protein contains cytochrome or a cytochrome complex. The present invention also relates to a method for forming a protein-immobilized membrane (14), and an enzyme-immobilized electrode and a biosensor (X1) provided with a protein-immobilized membrane (14). Preferably, the cell membrane homologous layer (14A) may contain a phospholipid polymer, and the protein (14B) may be CyGDH including an α subunit having a glucose dehydrogenase activity and cytochrome C having a function of electron transfer.
摘要翻译：本发明涉及固定在细胞膜同源层（14A）上的细胞膜同源层（14A）和蛋白质（14B）的蛋白质固定化膜（14），其中蛋白质含有细胞色素或细胞色素复合物。本发明还涉及一种形成固定蛋白质的膜（14）的方法，以及具有固定蛋白质的膜（14）的酶固定化电极和生物传感器（X1）。优选地，细胞膜同源层（14A）可以含有磷脂聚合物，并且蛋白质（14B）可以是包含具有葡萄糖脱氢酶活性的α亚基和具有电子转移功能的细胞色素C的CyGDH。

8. 发明申请

US20080280312A1 Mutant Glucose Dehydrogenase 有权
标题翻译：突变型葡萄糖脱氢酶
公开(公告)号：US20080280312A1
公开(公告)日：2008-11-13
申请号：US11587333
申请日：2005-04-22
申请人： Hideaki Yamaoka
发明人： Hideaki Yamaoka
IPC分类号： C12Q1/54 , C12N9/24 , C12N15/11 , C12M1/00 , C12N1/20
CPC分类号： C12N9/0006 , C12Q1/32
摘要： Substrate specificity for glucose of a glucose dehydrogenase having the amino acid sequence of SEQ ID NO: 13 is improved by substituting another amino acid residue for the amino acid residue at position 472 and/or 475.
摘要翻译：通过用另一个氨基酸残基取代位置472和/或475处的氨基酸残基来提高具有SEQ ID NO：13氨基酸序列的葡萄糖脱氢酶对葡萄糖的葡萄糖的底物特异性。

9. 发明申请

US20070034512A1 Biosensor and method for producing the same 审中-公开
标题翻译：生物传感器及其制造方法
公开(公告)号：US20070034512A1
公开(公告)日：2007-02-15
申请号：US10572423
申请日：2004-10-29
申请人： Hideaki Yamaoka , Tomomichi Tsujimoto
发明人： Hideaki Yamaoka , Tomomichi Tsujimoto
IPC分类号： G01N33/487
CPC分类号： C12Q1/004
摘要： The present invention provides a biosensor that can prevent a mediator from being affected by oxygen, thereby allowing an analyte in a sample solution to be measured rapidly and easily with high accuracy. The biosensor can be produced by providing a substrate having electrodes, applying a solvent containing a mediator, a surfactant, a buffer, and a layered inorganic compound to surfaces of the electrodes to form an inorganic gel layer for preventing natural oxidation of the mediator, and forming an enzyme reagent layer containing an oxidoreductase on the inorganic gel layer. In this biosensor, due to the inorganic gel layer, the mediator having been reduced by the reaction between an analyte and the oxidoreductase can be measured electrochemically, without being reoxidized by dissolved oxygen or the like.
摘要翻译：本发明提供一种可以防止介体受到氧的影响的生物传感器，从而能够以高精度快速，容易地测量样品溶液中的分析物。生物传感器可以通过提供具有电极的基板，将包含介体，表面活性剂，缓冲液和层状无机化合物的溶剂涂布到电极的表面，以形成用于防止介体的自然氧化的无机凝胶层，以及在无机凝胶层上形成含有氧化还原酶的酶试剂层。在该生物传感器中，由于无机凝胶层，通过分析物和氧化还原酶之间的反应而还原的介体可以电化学测定，而不会被溶解氧等重新氧化。

10. 发明申请

US20060035300A1 Method of measuring glucose concentration and glucose sensor with the use of glucose dehydrogenase 有权
标题翻译：使用葡萄糖脱氢酶测定葡萄糖浓度和葡萄糖传感器的方法
公开(公告)号：US20060035300A1
公开(公告)日：2006-02-16
申请号：US10518858
申请日：2003-06-16
申请人： Hideaki Yamaoka , Mitsuhiro Hoshijima , Tomomichi Tsujimoto
发明人： Hideaki Yamaoka , Mitsuhiro Hoshijima , Tomomichi Tsujimoto
IPC分类号： C12Q1/54
CPC分类号： G01N27/3272 , C12Q1/004 , C12Q1/006 , C12Q1/32
摘要： The present invention relates to a technique for measuring a glucose level by utilizing a reaction system containing an enzyme and an electron carrier. In accordance with the glucose level measuring method of the present invention, glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier. The present invention further provides a glucose sensor in which glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier.
摘要翻译：本发明涉及利用含有酶和电子载体的反应体系来测量葡萄糖水平的技术。根据本发明的葡萄糖水平测定方法，使用附着有细胞色素C的葡萄糖脱氢酶或来自属于伯克霍尔德氏菌属的微生物的葡萄糖脱氢酶作为酶，使用Ru化合物作为电子载体。本发明还提供一种葡萄糖传感器，其中使用连接有细胞色素C的葡萄糖脱氢酶或衍生自属于伯克霍尔德氏菌属的微生物的葡萄糖脱氢酶作为酶，并且使用Ru化合物作为电子载体。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式