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1. 发明申请

US20100001210A1 APERTURE BIOSENSOR WITH TRENCHES 有权
标题翻译：带孔的APERTURE生物传感器
公开(公告)号：US20100001210A1
公开(公告)日：2010-01-07
申请号：US12519849
申请日：2007-12-17
申请人： Derk J.W. Klunder , Maarten M.J.W. Van Herpen , Hendrik R. Stapert
发明人： Derk J.W. Klunder , Maarten M.J.W. Van Herpen , Hendrik R. Stapert
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L2200/025 , B01L2300/0654 , B01L2300/0816 , B01L2300/0887 , C12Q1/6837 , G01N21/47 , G01N21/554 , G01N21/658 , G01N2021/95676
摘要： A luminescence sensor, comprising a non-transparent substrate structure (2) having at least one aperture (5) intended to comprise an analyte and a transparent substrate structure (3), which is arranged to or adjacent said first structure (2). The aperture has a smallest lateral dimension, which is smaller than half the effective wavelength of an excitation radiation, such as light at a wavelength of 700 nm, resulting in an effective wavelength in water of about 538 nm. The transparent structure has a trench (4) with a surface portion provided with ligands with an affinity towards a target molecule. The trench results in that a luminophore attached to the target molecule will be positioned at the entrance surface of the aperture, where the excitation energy is largest.
摘要翻译：发光传感器，包括具有至少一个孔（5）的非透明衬底结构（2），所述孔旨在包括被分析物和透明衬底结构（3），所述透明衬底结构（3）布置在所述第一结构（2）上或邻近所述第一结构（2）。孔径具有最小的横向尺寸，其小于激发辐射的有效波长的一半，例如波长为700nm的光，导致在约538nm的水中的有效波长。透明结构具有沟槽（4），其表面部分具有对靶分子具有亲和性的配体。沟槽导致连接到靶分子的发光体将位于孔的入射表面处，其中激发能量最大。

2. 发明授权

US09176062B2 Aperture biosensor with trenches 有权
标题翻译：孔径生物传感器与沟槽
公开(公告)号：US09176062B2
公开(公告)日：2015-11-03
申请号：US12519849
申请日：2007-12-17
申请人： Derk J. W. Klunder , Maarten M. J. W. Van Herpen , Hendrik R. Stapert
发明人： Derk J. W. Klunder , Maarten M. J. W. Van Herpen , Hendrik R. Stapert
IPC分类号： G01N27/00 , G01N21/64
CPC分类号： G01N21/648 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L2200/025 , B01L2300/0654 , B01L2300/0816 , B01L2300/0887 , C12Q1/6837 , G01N21/47 , G01N21/554 , G01N21/658 , G01N2021/95676
摘要： A luminescence sensor, comprising a non-transparent substrate structure (2) having at least one aperture (5) intended to comprise an analyte and a transparent substrate structure (3), which is arranged to or adjacent said first structure (2). The aperture has a smallest lateral dimension, which is smaller than half the effective wavelength of an excitation radiation, such as light at a wavelength of 700 nm, resulting in an effective wavelength in water of about 538 nm. The transparent structure has a trench (4) with a surface portion provided with ligands with an affinity towards a target molecule. The trench results in that a luminophore attached to the target molecule will be positioned at the entrance surface of the aperture, where the excitation energy is largest.
摘要翻译：发光传感器，包括具有至少一个孔（5）的非透明衬底结构（2），所述孔旨在包括被分析物和透明衬底结构（3），所述透明衬底结构（3）布置在所述第一结构（2）上或邻近所述第一结构（2）。孔径具有最小的横向尺寸，其小于激发辐射的有效波长的一半，例如波长为700nm的光，导致在约538nm的水中的有效波长。透明结构具有沟槽（4），其表面部分具有对靶分子具有亲和性的配体。沟槽导致连接到靶分子的发光体将位于孔的入射表面处，其中激发能量最大。

3. 发明授权

US09134244B2 Wiregrid monitor device 有权
标题翻译：铁丝网监控设备
公开(公告)号：US09134244B2
公开(公告)日：2015-09-15
申请号：US12526547
申请日：2008-02-12
申请人： Derk J. W. Klunder , Maarten M. J. W. Van Herpen , Hendrik R. Stapert
发明人： Derk J. W. Klunder , Maarten M. J. W. Van Herpen , Hendrik R. Stapert
IPC分类号： G01N21/64 , B82Y15/00
CPC分类号： G01N21/648 , B82Y15/00 , G01N21/6445 , G01N21/6452
摘要： A device for monitoring radiation emitted by luminophores present in an analyte fluid of a wiregrid biosensor. The monitoring device comprises a non-polarized light source (41) for illuminating the wiregrid biosensor for exciting fluorescent labels arranged in the analyte fluid of said biosensor. A detector (71) detects radiation emitted by the labels after excitation. A polarizing filter (53) is arranged in between the transparent substrate and the detector for suppression of background emission radiation from labels positioned outside apertures in the wiregrid, in the analyte fluid.
摘要翻译：用于监测存在于丝网生物传感器的分析物流体中的发光体发射的辐射的装置。监测装置包括用于照亮丝网生物传感器以激发布置在所述生物传感器的分析物流体中的荧光标记的非偏振光源（41）。检测器（71）检测激励后由标签发射的辐射。偏振滤光器（53）布置在透明基板和检测器之间，用于抑制分析物流体中位于线网中的孔外部的标签的背景辐射辐射。

4. 发明授权

US08075841B2 Biosensor with one-dimensional sub-diffraction-limited apertures composed of a grid and a polarizer 有权
$Biosensor with one-dimensional sub-diffraction-limited apertures composed of a grid and a polarizer$
标题翻译：具有由栅格和偏振器组成的一维亚衍射限制孔的生物传感器
公开(公告)号：US08075841B2
公开(公告)日：2011-12-13
申请号：US12158347
申请日：2006-12-11
申请人： Maarten Van Herpen , Dirk J. Broer , Emiel Peeters , Derk J. W Klunder , Hendrik R. Stapert
发明人： Maarten Van Herpen , Dirk J. Broer , Emiel Peeters , Derk J. W Klunder , Hendrik R. Stapert
IPC分类号： G01N21/64 , G01N21/00 , G01J3/00 , G01N21/76
CPC分类号： G01N21/648 , Y10T436/11
摘要： A method and sensor for the detection of luminescence radiation generated by at least one luminophore is disclosed. In the context of the present invention the biosensor comprises a grid (120) defined as arrays of apertures with the apertures having a first dimension below and a second dimension above the diffraction limit of the excitation light (102) in a medium, a polarizer (115), and luminophores (117) positioned in a volume selected from the group consisting of: the volume inside the apertures of the grid (120), the volume in between the array of slits (120) and the polarizer (115) and a volume that extends into the polarizer (115), wherein the grid (120) providing a transmission axis extending in a first direction and the polarizer (115) providing a transmission axis extending in a second direction, the first direction and the second direction being substantially perpendicular with respect to each other, wherein the excitation radiation (102) is polarized such that it is substantially suppressed by one of the at least one grid (120) and polarizer (115) and substantially not suppressed by the other of the at least one grid (120) and polarizer (115).
摘要翻译：公开了一种用于检测由至少一个发光体产生的发光辐射的方法和传感器。在本发明的上下文中，生物传感器包括定义为孔阵列的格栅（120），孔格具有第一尺寸，第二维度高于介质中的激发光（102）的衍射极限，偏振器（ 115）和位于选自以下的体积中的发光体（117）：栅格（120）的孔内的体积，狭缝阵列（120）和偏振器（115）之间的体积和延伸到偏振器（115）的体积，其中提供沿第一方向延伸的透射轴的格栅（120）和提供沿第二方向延伸的透射轴的偏振器（115），第一方向和第二方向基本上垂直于彼此垂直，其中所述激发辐射（102）被极化，使得其基本上被所述至少一个格栅（120）和偏振器（115）中的一个所抑制，并且基本上不被所述o 该至少一个格栅（120）和偏振器（115）。

5. 发明申请

US20110039720A1 DEVICE AND METHOD FOR PARALLEL QUANTITATIVE ANALYSIS OF MULTIPLE NUCLEIC ACIDS 审中-公开
标题翻译：用于多个核酸并行定量分析的装置和方法
公开(公告)号：US20110039720A1
公开(公告)日：2011-02-17
申请号：US12809412
申请日：2008-12-16
申请人： Erik R. Vossenaar , Derk J. W. Klunder , Hendrik R. Stapert , Henk Van Houten , Bob Van Gemen , Erik M. H. P. Van Dijk
发明人： Erik R. Vossenaar , Derk J. W. Klunder , Hendrik R. Stapert , Henk Van Houten , Bob Van Gemen , Erik M. H. P. Van Dijk
IPC分类号： C40B30/04 , C40B60/12
CPC分类号： C12Q1/6818 , C12Q1/6851 , C12Q2525/301 , C12Q2565/1015 , C12Q2565/549 , C12Q2561/113
摘要： The present invention relates to a process for conducting real-time PCR, and to a device for conducting the method of the present invention. The invention is especially suited for the simultaneous identification and quantification of nucleic acids present in a sample, e.g. a biological sample. Further, this invention describes a method for simultaneous quantitative analysis of multiple nucleic acid sequences in a single compartment by using an integrated nucleic acid microarray combined with a highly surface-specific readout device. The invention relates to a device wherein a surface which is either part of the chamber surface or a surface that is created in the reaction chamber, such as bead surface, is coated with capture probes and in the same chamber, a PCR reaction takes place.
摘要翻译：本发明涉及一种用于进行实时PCR的方法以及用于实施本发明的方法的装置。本发明特别适用于同时鉴定和定量样品中存在的核酸，例如，生物样品。此外，本发明描述了通过使用与高表面特异性读出装置组合的整合的核酸微阵列来同时定量分析单个隔室中的多个核酸序列的方法。本发明涉及一种装置，其中作为室表面的一部分的表面或在反应室中产生的表面（例如珠粒表面）的表面涂覆有捕获探针，并且在相同的室中进行PCR反应。

6. 发明申请

US20100108908A1 WIREGRID MONITOR DEVICE 有权
标题翻译：线路监控器件
公开(公告)号：US20100108908A1
公开(公告)日：2010-05-06
申请号：US12526547
申请日：2008-02-12
申请人： Derk J.W. Klunder , Maarten M.J.W. Van Herpen , Hendrik R. Stapert
发明人： Derk J.W. Klunder , Maarten M.J.W. Van Herpen , Hendrik R. Stapert
IPC分类号： G01N21/64
CPC分类号： G01N21/648 , B82Y15/00 , G01N21/6445 , G01N21/6452
摘要： A device for monitoring radiation emitted by luminophores present in an analyte fluid of a wiregrid biosensor. The monitoring device comprises a non-polarized light source (41) for illuminating the wiregrid biosensor for exciting fluorescent labels arranged in the analyte fluid of said biosensor. A detector (71) detects radiation emitted by the labels after excitation. A polarizing filter (53) is arranged in between the transparent substrate and the detector for suppression of background emission radiation from labels positioned outside apertures in the wiregrid, in the analyte fluid.
摘要翻译：用于监测存在于丝网生物传感器的分析物流体中的发光体发射的辐射的装置。监测装置包括用于照亮丝网生物传感器以激发布置在所述生物传感器的分析物流体中的荧光标记的非偏振光源（41）。检测器（71）检测激励后由标签发射的辐射。偏振滤光器（53）布置在透明基板和检测器之间，用于抑制分析物流体中位于线网中的孔外部的标签的背景辐射辐射。

7. 发明申请

US20090001284A1 Biosensor with One-Dimensional Sub-Diffraction-Limited Apertures Composed of a Grid and a Polarizer 有权
$Biosensor with One-Dimensional Sub-Diffraction-Limited Apertures Composed of a Grid and a Polarizer$
标题翻译：具有由网格和偏振器组成的一维子衍射限制光圈的生物传感器
公开(公告)号：US20090001284A1
公开(公告)日：2009-01-01
申请号：US12158347
申请日：2006-12-11
申请人： Maarten van Herpen , Dirk J. Broer , Emiel Peeters , Derk J.W Klunder , Hendrik R. Stapert
发明人： Maarten van Herpen , Dirk J. Broer , Emiel Peeters , Derk J.W Klunder , Hendrik R. Stapert
IPC分类号： G01J1/58
CPC分类号： G01N21/648 , Y10T436/11
摘要： A method and sensor for the detection of luminescence radiation generated by at least one luminophore is disclosed. In the context of the present invention the biosensor comprises a grid (120) defined as arrays of apertures with the apertures having a first dimension below and a second dimension above the diffraction limit of the excitation light (102) in a medium, a polarizer (115), and luminophores (117) positioned in a volume selected from the group consisting of: the volume inside the apertures of the grid (120), the volume in between the array of slits (120) and the polarizer (115) and a volume that extends into the polarizer (115), wherein the grid (120) providing a transmission axis extending in a first direction and the polarizer (115) providing a transmission axis extending in a second direction, the first direction and the second direction being substantially perpendicular with respect to each other, wherein the excitation radiation (102) is polarized such that it is substantially suppressed by one of the at least one grid (120) and polarizer (115) and substantially not suppressed by the other of the at least one grid (120) and polarizer (115).
摘要翻译：公开了一种用于检测由至少一个发光体产生的发光辐射的方法和传感器。在本发明的上下文中，生物传感器包括定义为孔阵列的格栅（120），孔格具有第一尺寸，第二维度高于介质中的激发光（102）的衍射极限，偏振器（ 115）和位于选自以下的体积中的发光体（117）：栅格（120）的孔内的体积，狭缝阵列（120）和偏振器（115）之间的体积和延伸到偏振器（115）的体积，其中提供沿第一方向延伸的透射轴的格栅（120）和提供沿第二方向延伸的透射轴的偏振器（115），第一方向和第二方向基本上垂直于彼此垂直，其中所述激发辐射（102）被极化，使得其基本上被所述至少一个格栅（120）和偏振器（115）中的一个所抑制，并且基本上不被所述o 该至少一个格栅（120）和偏振器（115）。

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