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1. 发明授权

US09334477B2 Method for collecting nucleated red blood cells via density-gradient centrifugation utilizing changes in blood cell density 有权
标题翻译：通过使用血细胞密度变化的密度梯度离心法收集有核红细胞的方法
公开(公告)号：US09334477B2
公开(公告)日：2016-05-10
申请号：US13700947
申请日：2011-02-21
申请人： Yuzuru Takamura , Kotaro Idegami , Mieko Kogi , Haruo Takabayashi
发明人： Yuzuru Takamura , Kotaro Idegami , Mieko Kogi , Haruo Takabayashi
IPC分类号： B03D3/00 , C12N5/078 , C12N5/00 , G01N33/49 , G01N33/80 , C12Q1/68
CPC分类号： C12N5/0641 , B03D3/00 , C12N5/0087 , C12Q1/6806 , C12Q1/6883 , C12Q2600/118 , C12Q2600/158 , G01N33/491 , G01N33/80 , C12Q2523/32 , C12Q2527/15 , C12Q2543/10
摘要： Method for concentrating and collecting small quantities of fetal nucleated red blood cells contained in the maternal blood. The method for concentrating and collecting nucleated red blood cells from the maternal blood comprises: (i) subjecting the maternal blood to a first density-gradient centrifugation and collecting a cell fraction containing nucleated red blood cells; (ii) treating the cell fraction containing nucleated red blood cells so as to selectively changes the density of the nucleated red blood cells from that of the white blood cells; and (iii) subjecting the treated cell fraction containing the nucleated red blood cells to a second density-gradient centrifugation so as to collect a fraction containing nucleated red blood cells.
摘要翻译：浓缩和收集母体血液中少量胎儿有核红细胞的方法。用于从母体血液中浓缩和收集有核红细胞的方法包括：（i）使母体血液进行第一密度梯度离心并收集含有核红细胞的细胞部分; （ii）处理含有核红细胞的细胞部分，以选择性地改变有核红细胞的密度与白细胞的密度; 和（iii）将含有有核红细胞的经处理的细胞级分进行第二密度梯度离心，以收集含有核红细胞的级分。

2. 发明申请

US20120301867A1 RECOVERING NUCLEATED RED BLOOD CELLS AND METHOD FOR CONCENTRATING AND RECOVERING NUCLEATED RED BLOOD CELLS 审中-公开
标题翻译：恢复核红细胞和浓缩和回收核红细胞的方法
公开(公告)号：US20120301867A1
公开(公告)日：2012-11-29
申请号：US13393854
申请日：2010-09-02
申请人： Takeshi Kumo , Yuzuru Takamura , Haruo Takabayashi
发明人： Takeshi Kumo , Yuzuru Takamura , Haruo Takabayashi
IPC分类号： C12M1/26 , C12N5/078
CPC分类号： B01L3/502746 , B01L3/502723 , B01L3/502738 , B01L3/502753 , B01L2200/0652 , B01L2300/0864 , B01L2400/0638 , B01L2400/0655 , B01L2400/082
摘要： Provided is a chip or the like having a particulate concentrating mechanism such as a mechanism that can selectively concentrate nucleated red blood cells contained in maternal blood and derived from a fetus and can collect the concentrated liquid rich in nucleated red blood cells, and also provided is a nucleated red blood cell concentrating/collecting method. A micro-channel chip for concentrating nucleated red blood cells has an inlet-side channel, an outlet-side channel, and a separation narrow channel provided between the inlet-side channel and the outlet-side channel. The separation narrow channel has an inner wall having a dimension through which non-nucleated red blood cells easily pass and nucleated red blood cells hardly pass, and has a means for deforming or moving part of the inner wall of the channel to have a dimension through which nucleated red blood cells easily pass. The following are also provided: a method for collecting a liquid in which nucleated red blood cells obtained by using this chip are concentrated; and a micro-channel chip for concentrating particulates other than for concentrating nucleated red blood cells.
摘要翻译：提供了具有颗粒聚集机构的芯片等，其可以选择性地浓缩母体血液中衍生的来自胎儿的有核红细胞，并且可以收集富含有核红细胞的浓缩液，并且还提供有核红细胞浓缩/收集方法。用于浓缩有核红细胞的微通道芯片具有入口侧通道，出口侧通道和设置在入口侧通道和出口侧通道之间的分离窄通道。分离窄通道具有内壁，该内壁具有非成核红细胞容易通过且有核的红细胞难以通过的尺寸，并且具有使通道内壁的一部分变形或移动以使其具有尺寸的装置其核红细胞容易通过。还提供以下方法：收集通过使用该芯片得到的有核红细胞的液体的收集方法; 以及用于浓缩除了用于浓缩有核红细胞之外的微粒的微通道芯片。

3. 发明申请

US20130072402A1 METHOD FOR COLLECTING NUCLEATED RED BLOOD CELLS VIA DENSITY-GRADIENT CENTRIFUGATION UTILIZING CHANGES IN BLOOD CELL DENSITY 有权
标题翻译：通过密度梯度离心收集核型红血细胞的方法利用血细胞密度变化
公开(公告)号：US20130072402A1
公开(公告)日：2013-03-21
申请号：US13700947
申请日：2011-02-21
申请人： Yuzuru Takamura , Kotaro Idegami , Mieko Kogi , Haruo Takabayashi
发明人： Yuzuru Takamura , Kotaro Idegami , Mieko Kogi , Haruo Takabayashi
IPC分类号： C12N5/078
CPC分类号： C12N5/0641 , B03D3/00 , C12N5/0087 , C12Q1/6806 , C12Q1/6883 , C12Q2600/118 , C12Q2600/158 , G01N33/491 , G01N33/80 , C12Q2523/32 , C12Q2527/15 , C12Q2543/10
摘要： This invention provides a method for concentrating and collecting small quantities of fetal nucleated red blood cells contained in the maternal blood. The method for concentrating and collecting nucleated red blood cells from the maternal blood comprises: (i) subjecting the maternal blood to a first density-gradient centrifugation and collecting a cell fraction containing nucleated red blood cells; (ii) treating the cell fraction containing nucleated red blood cells so as to selectively changes the density of the nucleated red blood cells from that of the white blood cells; and (iii) subjecting the treated cell fraction containing the nucleated red blood cells to a second density-gradient centrifugation so as to collect a fraction containing nucleated red blood cells.
摘要翻译：本发明提供了一种用于浓缩和收集母体血液中含有的少量胎儿有核红细胞的方法。用于从母体血液中浓缩和收集有核红细胞的方法包括：（i）使母体血液进行第一密度梯度离心并收集含有核红细胞的细胞部分; （ii）处理含有核红细胞的细胞部分，以选择性地改变有核红细胞的密度与白细胞的密度; 和（iii）将含有有核红细胞的经处理的细胞级分进行第二密度梯度离心，以收集含有核红细胞的级分。

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