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1. 发明授权

US09238840B2 Method for single cell genome analysis and kit therefor 有权
标题翻译：单细胞基因组分析方法及其试剂盒
公开(公告)号：US09238840B2
公开(公告)日：2016-01-19
申请号：US13976845
申请日：2011-12-29
申请人： Xuyang Yin , Li Bao , Xun Xu , Hanjie Wu , Xiaoyu Liu , Xiuqing Zhang , Huanming Yang
发明人： Xuyang Yin , Li Bao , Xun Xu , Hanjie Wu , Xiaoyu Liu , Xiuqing Zhang , Huanming Yang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6888 , C12Q1/6806 , C12Q1/6851 , C12Q1/6869 , C12Q1/6876 , C12Q2600/166 , C12Q2531/119 , C12Q2545/101
摘要： A method for analyzing a genome of a single cell is provided, and a kit is also provided. The method for analyzing the genome of the single cell may comprise separating and lysing the single cell to obtain a whole-genome DNA of the cell; subjecting the whole-genome DNA to a whole-genome amplification to obtain a whole-genome amplification product; performing a PCR amplification using the whole-genome amplification product as template and using housekeeping-gene-specific primers to detect the housekeeping gene of the whole-genome amplification product; and determining whether the whole genome amplification product meets a requirement for sequencing based on the detection result, wherein a uniform distribution of the amplification product in each chromosome is an indication of the amplification product meeting the requirement for sequencing.
摘要翻译：提供了用于分析单细胞的基因组的方法，并且还提供了试剂盒。用于分析单细胞基因组的方法可以包括分离和裂解单细胞以获得细胞的全基因组DNA; 对全基因组DNA进行全基因组扩增以获得全基因组扩增产物; 使用全基因组扩增产物作为模板进行PCR扩增，并使用管家基因特异性引物检测全基因组扩增产物的管家基因; 并且基于检测结果确定全基因组扩增产物是否满足测序要求，其中扩增产物在每个染色体中的均匀分布是满足测序要求的扩增产物的指示。

2. 发明申请

US20140017683A1 METHOD FOR SINGLE CELL GENOME ANALYSIS AND KIT THEREFOR 有权
标题翻译：单细胞基因组分析方法及其制备方法
公开(公告)号：US20140017683A1
公开(公告)日：2014-01-16
申请号：US13976845
申请日：2011-12-29
申请人： Xuyang Yin , Li Bao , Xun Xu , Hanjie Wu , Xiaoyu Liu , Xiuqing Zhang , Huanming Yang
发明人： Xuyang Yin , Li Bao , Xun Xu , Hanjie Wu , Xiaoyu Liu , Xiuqing Zhang , Huanming Yang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6888 , C12Q1/6806 , C12Q1/6851 , C12Q1/6869 , C12Q1/6876 , C12Q2600/166 , C12Q2531/119 , C12Q2545/101
摘要： A method for analyzing a genome of a single cell is provided, and a kit is also provided. The method for analyzing the genome of the single cell may comprise separating and lysing the single cell to obtain a whole-genome DNA of the cell; subjecting the whole-genome DNA to a whole-genome amplification to obtain a whole-genome amplification product; performing a PCR amplification using the whole-genome amplification product as template and using housekeeping-gene-specific primers to detect the housekeeping gene of the whole-genome amplification product; and determining whether the whole genome amplification product meets a requirement for sequencing based on the detection result, wherein a uniform distribution of the amplification product in each chromosome is an indication of the amplification product meeting the requirement for sequencing.
摘要翻译：提供了用于分析单细胞的基因组的方法，并且还提供了试剂盒。用于分析单细胞基因组的方法可以包括分离和裂解单细胞以获得细胞的全基因组DNA; 对全基因组DNA进行全基因组扩增以获得全基因组扩增产物; 使用全基因组扩增产物作为模板进行PCR扩增，并使用管家基因特异性引物检测全基因组扩增产物的管家基因; 并且基于检测结果确定全基因组扩增产物是否满足测序要求，其中扩增产物在每个染色体中的均匀分布是满足测序要求的扩增产物的指示。

3. 发明申请

US20150299753A1 WHOLE GENOME AMPLIFICATION METHOD AND APPLICATION THEREOF 审中-公开
标题翻译：全基因扩增方法及其应用
公开(公告)号：US20150299753A1
公开(公告)日：2015-10-22
申请号：US14378935
申请日：2012-03-30
申请人： Kui WU , Hanjie WU , Yong HOU , Xun XU , Jun WANG
发明人： Kui WU , Hanjie WU , Yong HOU , Xun XU , Jun WANG
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12P19/34 , C12Q1/6806 , C12Q1/686 , C12Q2537/149
摘要： Provided are a whole genome sample amplification method, a whole genome sequencing method, and a method for determining whether an abnormal state occurs in a whole genome, a whole genome sample amplification apparatus, a whole genome sequencing device, and a system for determining whether an abnormal state occurs in a whole genome. The whole genome sample amplification method comprises: subjecting a whole genome sample to a first amplification reaction, so as to obtain a first amplification product; and subjecting the first amplification product to a second amplification reaction, so as to obtain a second amplification product. The first amplification reaction is one of the PCR-based amplification reaction and the isothermal amplification reaction, and the second amplification reaction is the other of the PCR-based amplification reaction and the isothermal amplification reaction.
摘要翻译：提供了全基因组样品扩增方法，全基因组测序方法以及用于确定全基因组中是否发生异常状态的方法，全基因组样品扩增装置，全基因组测序装置和用于确定是否异常状态发生在整个基因组中。全基因组样品扩增方法包括：对全基因组样品进行第一次扩增反应，得到第一扩增产物; 并对第一扩增产物进行第二次扩增反应，得到第二扩增产物。第一次扩增反应是基于PCR的扩增反应和等温扩增反应之一，第二次扩增反应是基于PCR的扩增反应和等温扩增反应中的另一个。

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