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    • 3. 发明授权
    • Methods for diagnosis of Clostridium difficile and methods and vectors for recombinant toxin expression
    • 艰难梭菌诊断方法及重组毒素表达的方法和载体
    • US08206940B2
    • 2012-06-26
    • US12992330
    • 2009-05-15
    • Hanping FengSaul TziporiGuilin Yang
    • Hanping FengSaul TziporiGuilin Yang
    • G01N33/53
    • G01N33/56911G01N33/5014G01N33/5055G01N2333/33
    • Cell-based methods for rapid real time assay of a presence of Clostridium difficile toxin and/or cells are provided, using an assay having a toxin-enhancing antibody and a sensitive cell line carrying FcyR receptors, and kits for this assay. An ultrasensitive cell based immunocytotoxicity assay for detecting less then 1 pg/ml of C. difficile toxins in clinical samples. A TcdA-specific monoclonal antibody, AIH3, was found to significantly enhance the cytotoxicity of TcdA to macrophages and monocytes. The AIH3-dependent enhancement of glucosyltransferase activity, cytoskeleton disruption, and TNF-a production induced by TcdA was demonstrated also in RAW 264.7 cells. Methods for high level recombinant expression of C. difficile toxins in Bacillus cells, and vectors for expression, strains of Bacillus carrying the vectors are provided.
    • 使用具有毒素增强抗体和携带FcyR受体的敏感细胞系的测定法和用于该测定的试剂盒,提供了用于快速实时测定艰难梭菌毒素和/或细胞存在的基于细胞的方法。 用于在临床样品中检测少于1pg / ml的艰难梭菌毒素的基于超敏感的基于细胞的免疫细胞毒性测定。 发现TcdA特异性单克隆抗体AIH3显着增强TcdA对巨噬细胞和单核细胞的细胞毒性。 在RAW 264.7细胞中也证明了由TcdA诱导的葡萄糖基转移酶活性,细胞骨架破坏和TNF-α产生的AIH3依赖性增强。 提供了在芽孢杆菌细胞中高水平重组表达艰难梭菌毒素的方法和用于表达载体的载体载体的芽孢杆菌菌株。
    • 4. 发明申请
    • PROCESS FOR PRETREATING ORGANIC EXTRACTANTS AND ITS PRODUCT AND APPLICATION
    • 预处理有机提取物及其产品和应用的方法
    • US20100003176A1
    • 2010-01-07
    • US12526436
    • 2008-02-02
    • Xiaowei HuangHongwei LiZhiqi LongXinlin PengDali CuiGuilin YangXinghua LuoNa ZhaoYongqi Zhang
    • Xiaowei HuangHongwei LiZhiqi LongXinlin PengDali CuiGuilin YangXinghua LuoNa ZhaoYongqi Zhang
    • C01F17/00
    • C22B3/0067B01D11/0288B01D11/0492C01F17/0006C22B3/0005C22B3/0044C22B59/00Y02P10/234
    • A process for pretreating organic extractants and its product and application in SX separation of rare earth. The pretreating method is that extractant and rare earth solution are mixed with powder or slurry of alkaline earth metal compound containing magnesium and/or calcium to realize pre-extraction, or the organic extractant are mixed with rare earth carbonate slurry to realize pre-extraction. When rare earth ion in aqueous phase is extracted into organic phase, the exchanged hydrogen ions enter into aqueous phase and dissolve the alkaline earth metal compound or the rare earth carbonate which helps to keep the acidity equilibrium of the system. The obtained organic extractant loaded with rare earth is used for unsaponificated SX separation of rare earth.With this method, there is no need to saponificate organic extractant with liquid ammonia or alkali, and there is no ammonia-nitrogen wastewater produced. So separation cost decrease at a large scale and a lot of the cost to treat the three wastes is cut. This method is applicable to SX separation for all rare earth elements in chloride, sulphate and nitrate system and has advantages of low investment and high profit.
    • 一种用于预处理有机萃取剂及其产品和在稀土SX分离中的应用的方法。 预处理方法是将萃取剂和稀土溶液与含有镁和/或钙的碱土金属化合物的粉末或浆料混合以实现预萃取,或者将有机萃取剂与稀土碳酸盐浆料混合以实现预萃取。 当水相中的稀土离子萃取到有机相中时,交换的氢离子进入水相并溶解碱土金属化合物或稀土碳酸盐,有助于保持体系的酸度平衡。 将所得到的装有稀土的有机萃取剂用于稀土的未分配的SX分离。 通过这种方法,不需要用液体氨或碱对有机萃取剂进行皂化,不产生氨氮废水。 因此,分离成本大幅度地下降,处理三种废物的成本大幅度下降。 该方法适用于氯化物,硫酸盐和硝酸盐体系中所有稀土元素的SX分离,具有投资低,利润高的优点。
    • 6. 发明申请
    • Methods for diagnosis of Clostridium difficile and methods and vectors for recombinant toxin expression
    • 艰难梭菌诊断方法及重组毒素表达的方法和载体
    • US20110065123A1
    • 2011-03-17
    • US12992330
    • 2009-05-15
    • Hanping FengSaul TziporiGuilin Yang
    • Hanping FengSaul TziporiGuilin Yang
    • G01N33/53C12Q1/04C12P1/04C12N15/63C12N5/07
    • G01N33/56911G01N33/5014G01N33/5055G01N2333/33
    • Cell-based methods for rapid real time assay of a presence of Clostridium difficile toxin and/or cells are provided, using an assay having a toxin-enhancing antibody and a sensitive cell line carrying FcyR receptors, and kits for this assay. An ultrasensitive cell based immunocytotoxicity assay for detecting less then 1 pg/ml of C. difficile toxins in clinical samples. A TcdA-specific monoclonal antibody, AIH3, was found to significantly enhance the cytotoxicity of TcdA to macrophages and monocytes. The AIH3-dependent enhancement of glucosyltransferase activity, cytoskeleton disruption, and TNF-a production induced by TcdA was demonstrated also in RAW 264.7 cells. Methods for high level recombinant expression of C. difficile toxins in Bacillus cells, and vectors for expression, strains of Bacillus carrying the vectors are provided.
    • 使用具有毒素增强抗体和携带FcyR受体的敏感细胞系的测定法和用于该测定的试剂盒,提供了用于快速实时测定艰难梭菌毒素和/或细胞存在的基于细胞的方法。 用于在临床样品中检测少于1pg / ml的艰难梭菌毒素的基于超敏感的基于细胞的免疫细胞毒性测定。 发现TcdA特异性单克隆抗体AIH3显着增强TcdA对巨噬细胞和单核细胞的细胞毒性。 在RAW 264.7细胞中也证明了由TcdA诱导的葡萄糖基转移酶活性,细胞骨架破坏和TNF-α产生的AIH3依赖性增强。 提供了在芽孢杆菌细胞中高水平重组表达艰难梭菌毒素的方法和用于表达载体的载体载体的芽孢杆菌菌株。
    • 8. 发明授权
    • Method of precipitation of metal ions
    • 金属离子析出方法
    • US08808660B2
    • 2014-08-19
    • US13145632
    • 2010-02-09
    • Xiaowei HuangZhiqi LongHongwei LiDali CuiXinlin PengGuilin YangYongke HouChunmei WangShunli Zhang
    • Xiaowei HuangZhiqi LongHongwei LiDali CuiXinlin PengGuilin YangYongke HouChunmei WangShunli Zhang
    • C01B31/24
    • C22B3/44C04B22/064C04B22/066C22B59/00Y02P10/234
    • The present invention relates to a method of precipitation of metal ions. Mineral(s), oxide(s), hydroxide(s) of magnesium and/or calcium are adopted as raw materials, and the raw material(s) is processed through at least one step of calcination, slaking, or carbonization to produce aqueous solution(s) of magnesium bicarbonate and/or calcium bicarbonate, and then the solution(s) is used as precipitant(s) to deposit rare earth, such as nickel, cobalt, iron, aluminum, gallium, indium, manganese, cadmium, zirconium, hafnium, strontium, barium, copper and zinc ions. And at least one of metal carbonates, hydroxides or basic carbonates is obtained, or furthermore the obtained products are calcined to produce metal oxides. The invention takes the cheap calcium and/or magnesium minerals or their oxides, hydroxides with low purity as raw materials to instead common precipitants such as ammonium bicarbonate and sodium carbonate etc. The calcium, magnesium, carbon dioxide etc are efficiently and circularly used, and the environment pollution by ammonium-nitrogen wastewater, high concentration salts wastewater is avoided, and both of the discharge of greenhouse gas carbon dioxide and the production cost of metal are decreased.
    • 本发明涉及一种沉淀金属离子的方法。 采用矿物,氧化物,镁和/或钙的氢氧化物作为原料,通过至少一个煅烧,去渣或碳化步骤处理原料以产生水溶液 碳酸氢钙和/或碳酸氢钙的溶液,然后使用该溶液作为沉淀剂沉积稀土,例如镍,钴,铁,铝,镓,铟,锰,镉, 锆,铪,锶,钡,铜和锌离子。 并且获得金属碳酸盐,氢氧化物或碱性碳酸盐中的至少一种,或者进一步将所得产物煅烧以产生金属氧化物。 本发明以廉价的钙和/或镁矿物或其氧化物,低纯度的氢氧化物为原料,代替普通的沉淀剂如碳酸氢铵和碳酸钠等。钙,镁,二氧化碳等有效和循环使用, 避免了氮铵废水,高浓度盐水等环境污染,温室气体二氧化碳的排放和金属的生产成本均下降。