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1. 发明授权

US11492597B2 Method for establishing colorectal cancer HK2 reporter gene cell line 有权
公开(公告)号：US11492597B2
公开(公告)日：2022-11-08
申请号：US16679759
申请日：2019-11-11
申请人： Guangdong Medical University
发明人： Hongbing Yu , Xin Liu
IPC分类号： C12N5/09 , C12Q1/6897 , C12N15/90
摘要： The present invention discloses a method for establishing a colorectal cancer HK2 reporter gene cell line, specifically including: firstly, designing a site-specific sgRNA sequence of an HK2 gene, and cloning same into a PX459 plasmid; integrating a homologous recombination sequence of an HK2 gene and a green fluorescent protein DNA fragment (EGFP), and transforming the plasmid and the integrated fragment together into a colorectal cancer cell line HCT116 by electroporation; and performing signal cell screening through a flow cytometer to obtain EGFP-expressing cells, and amplifying a monoclonal cell line; and identifying a positive HK2 reporter gene cell line through PCR identification and Western blot, among screened EGFP-expressing cell lines. The colorectal cancer cell line HK2 gene and EGFP are co-expressed, and the expression level of the EGFP is highly consistent with that of the HK2 gene. Therefore, the expression level of the HK2 gene can be accurately determined by detecting a change in the expression level of the EGFP. The method for establishing a cell line in the present invention are simple, easy to implement and efficient, and a gene site can be precisely positioned.

2. 发明申请

US20210130791A1 METHOD FOR ESTABLISHING COLORECTAL CANCER HK2 REPORTER GENE CELL LINE 有权
公开(公告)号：US20210130791A1
公开(公告)日：2021-05-06
申请号：US16679759
申请日：2019-11-11
申请人： Guangdong Medical University
发明人： Hongbing Yu , Xin Liu
IPC分类号： C12N5/09 , C12N15/90 , C12Q1/6897
摘要： The present invention discloses a method for establishing a colorectal cancer HK2 reporter gene cell line, specifically including: firstly, designing a site-specific sgRNA sequence of an HK2 gene, and cloning same into a PX459 plasmid; integrating a homologous recombination sequence of an HK2 gene and a green fluorescent protein DNA fragment (EGFP), and transforming the plasmid and the integrated fragment together into a colorectal cancer cell line HCT116 by electroporation; and performing signal cell screening through a flow cytometer to obtain EGFP-expressing cells, and amplifying a monoclonal cell line; and identifying a positive HK2 reporter gene cell line through PCR identification and Western blot, among screened EGFP-expressing cell lines. The colorectal cancer cell line HK2 gene and EGFP are co-expressed, and the expression level of the EGFP is highly consistent with that of the HK2 gene. Therefore, the expression level of the HK2 gene can be accurately determined by detecting a change in the expression level of the EGFP. The method for establishing a cell line in the present invention are simple, easy to implement and efficient, and a gene site can be precisely positioned.

3. 发明申请

US20210047370A1 A functional polypeptide and its application thereof in preparing medicament for preventing and treating pulmonary fibrosis 有权
公开(公告)号：US20210047370A1
公开(公告)日：2021-02-18
申请号：US16331995
申请日：2018-06-16
申请人： Guangdong Medical University
发明人： Haitao Zhang , Huajun Yu , Jun Wu , Hui Luo
IPC分类号： C07K7/08 , A61P11/00
摘要： The present invention discloses a functional polypeptide which is 1) a polypeptide having the amino acid sequence of SEQ ID No. 1; or 2) a polypeptide having a substitution or deletion or addition of one or several amino acids from the sequence shown in SEQ ID No. 1 that has the same or similar function. The invention also discloses the utility of the described functional polypeptide. The functional polypeptide of the present invention can significantly inhibit bleomycin-induced pulmonary fibrosis formation and has a remarkable effect of preventing and treating pulmonary fibrosis.

4. 发明申请

US20200208118A1 METHODS FOR ESTABLISHING COLORECTAL CANCER p73 REPORTER GENE CELL LINE 审中-公开
公开(公告)号：US20200208118A1
公开(公告)日：2020-07-02
申请号：US16680920
申请日：2019-11-12
申请人： Guangdong Medical University
发明人： Hongbing Yu , Xin Liu , Cuifang Han
IPC分类号： C12N5/09 , C12N15/85 , C12N5/071 , G01N33/50
摘要： The present invention discloses a method for establishing a colorectal cancer p73 reporter gene cell line, specifically including: first designing a site-specific sgRNA sequence of a p73 gene and cloning same into a plasmid PX459; integrating a homologous recombination sequence of the p73 gene and a green fluorescent protein DNA fragment (EGFP), and transforming the plasmid and the integrated fragment together into a colorectal cancer cell line HCT116 by electroporation; performing signal cell screening through a flow cytometer to obtain EGFP-expressing cells, and amplifying a monoclonal cell line; and identifying a positive p73 reporter gene cell line through PCR identification and Western blot, among screened EGFP-expressing cell lines. The colorectal cancer cell line p73 gene and the EGFP are co-expressed, and the expression level of the EGFP is highly consistent with that of the p73 gene. Therefore, the expression level of the p73 gene can be accurately determined by detecting changes in the expression level of the EGFP. The method for establishing the cell line in the present invention is simple, easy to implement, high in efficiency and precise in gene site positioning.

5. 发明公开

US20240082285A1 APPLICATION OF CHEBULINIC ACID IN PREPARATION OF ANTI-ROTAVIRUS DRUGS 审中-公开
公开(公告)号：US20240082285A1
公开(公告)日：2024-03-14
申请号：US18510757
申请日：2023-11-16
申请人： GUANGDONG MEDICAL UNIVERSITY
发明人： Lijun Song , Wenchang Zhao , Yupei Qian , Yuxuan Feng , Yujing Zhou
IPC分类号： A61K31/7048 , A61P31/14
CPC分类号： A61K31/7048 , A61P31/14
摘要： The present invention discloses an application of chebulinic acid in preparation of anti-rotavirus drugs, which belongs to the technical field of anti-rotavirus drugs. Chebulinic acid has the effect of resisting rotavirus biosynthesis, has no effect of resistance to rotavirus adsorption and direct killing, and can inhibit the gene expression of VP6 to play an anti-RV role. Network pharmacology analysis indicates that chebulinic acid acts on rotavirus-related genes. Compared with the prior art, the present invention has the beneficial effects that: the present invention relates to an application of chebulinic acid in preparation of anti-rotavirus drugs. In the application of chebulinic acid in preparation of anti-rotavirus drugs, the applicant first finds through research that chebulinic acid has an anti-rotavirus effect, fills the gap of anti-rotavirus drugs, and has profound significance and value, wherein the research shows that the molar concentration of chebulinic acid is 1 μM-10 μM in the anti-rotavirus drugs.

6. 发明公开

US20240099978A1 Composite Nano-agent for Combating Drug-fast Bacteria and Preparation Method Thereof 审中-公开
公开(公告)号：US20240099978A1
公开(公告)日：2024-03-28
申请号：US18530276
申请日：2023-12-06
申请人： Guangdong Medical University
发明人： Xingyan Liu , Yining Liu
IPC分类号： A61K9/127 , A61K33/38 , A61K47/10 , A61K47/32
CPC分类号： A61K9/1277 , A61K33/38 , A61K47/10 , A61K47/32
摘要： Disclosed is a composite nano-agent for combating drug-fast bacteria and a preparation method thereof. The solution comprises several steps of operation that a silver salt is reduced into silver nanoparticles; then, phosphatidylcholine (PC) and cholesterol are mixed and added into water, and ultrasonic treatment is carried out to form liposomes; the silver nanoparticles and the liposomes are then mixed and an ultrasonic processor is used to uniformly mix a mixture by adding polyethylene glycol (PEG); an anti-bacterial experiment is carried out on the prepared reagent to determine a sterilization effect and a minimal inhibitory concentration (MIC) thereof.

7. 发明公开

US20230407364A1 LONG-ACTING RECOMBINANT INTERLEUKIN-18 BINDING PROTEIN AND PRODUCTION METHOD AND APPLICATION THEREOF 审中-公开
公开(公告)号：US20230407364A1
公开(公告)日：2023-12-21
申请号：US18332489
申请日：2023-06-09
申请人： Guangdong Medical University
发明人： Yechen Xiao , Lei Guo , Xiuze Chen
IPC分类号： C12P21/02 , C07K16/24 , C12N15/70
CPC分类号： C12P21/02 , C07K16/244 , C12N15/70 , C12N2800/101
摘要： A long-acting recombinant interleukin-18 binding protein (IL-18BP) and its production method and application are provided, belonging to the field of biotechnology. The recombinant IL-18BP includes a sequence of human IL-18BP isoform a and a sequence of human IgG-Fc, and an amino acid sequence of the recombinant IL-18BP is shown in SEQ ID NO: 1. In the preparation method of the recombinant IL-18BP, an encoding gene expressing a fusion protein is inserted into a cell expression vector and the vector is introduced into prokaryotic cells to express the recombinant IL-18BP. A prokaryotic expression system is used to express the recombinant IL-18BP, promoting correct protein folding, enhancing protein stability, reducing in vivo degradation rate, and enhancing its biological activity.

8. 发明授权

US11225504B2 Functional polypeptide and its application thereof in preparing medicament for preventing and treating pulmonary fibrosis 有权
公开(公告)号：US11225504B2
公开(公告)日：2022-01-18
申请号：US16331995
申请日：2018-06-16
申请人： Guangdong Medical University
发明人： Haitao Zhang , Huajun Yu , Jun Wu , Hui Luo
IPC分类号： C07K7/08 , A61P11/00 , A61K38/00
摘要： The present invention discloses a functional polypeptide which is 1) a polypeptide having the amino acid sequence of SEQ ID No. 1; or 2) a polypeptide having a substitution or deletion or addition of one or several amino acids from the sequence shown in SEQ ID No. 1 that has the same or similar function. The invention also discloses the utility of the described functional polypeptide. The functional polypeptide of the present invention can significantly inhibit bleomycin-induced pulmonary fibrosis formation and has a remarkable effect of preventing and treating pulmonary fibrosis.

9. 发明授权

US11992475B1 Application of Z-ligustilide in resisting rotaviruses 有权
公开(公告)号：US11992475B1
公开(公告)日：2024-05-28
申请号：US18544621
申请日：2023-12-19
申请人： GUANGDONG MEDICAL UNIVERSITY
发明人： Lijun Song , Wenchang Zhao
IPC分类号： A61K31/365 , A61P31/14
CPC分类号： A61K31/365 , A61P31/14
摘要： The present invention discloses an application of Z-ligustilide in resisting rotaviruses, and relates to the technical field of medicines. Z-ligustilide has no drug toxicity to cells at 60-95 μM and can effectively inhibit rotavirus biosynthesis at 70-85 μM. Z-ligustilide plays a role in resisting rotavirus biosynthesis by inhibiting structural protein VP6, and has no effect of adsorbing and directly killing rotaviruses, which provides a new way for resisting rotaviruses.

10. 发明授权

US11667893B2 Methods for establishing colorectal cancer p73 reporter gene cell line 有权
公开(公告)号：US11667893B2
公开(公告)日：2023-06-06
申请号：US16680920
申请日：2019-11-12
申请人： Guangdong Medical University
发明人： Hongbing Yu , Xin Liu , Cuifang Han
IPC分类号： C07H21/02 , C07H21/04 , C12N5/09 , C12N15/85 , C12N5/071 , G01N33/50
CPC分类号： C12N5/0693 , C12N5/0679 , C12N15/85 , G01N33/5011 , G01N33/5044 , C12N2310/20 , C12N2320/10 , C12N2800/107 , G01N2500/02 , G01N2500/10
摘要： The present invention discloses a method for establishing a colorectal cancer p73 reporter gene cell line, specifically including: first designing a site-specific sgRNA sequence of a p73 gene and cloning same into a plasmid PX459; integrating a homologous recombination sequence of the p73 gene and a green fluorescent protein DNA fragment (EGFP), and transforming the plasmid and the integrated fragment together into a colorectal cancer cell line HCT116 by electroporation; performing signal cell screening through a flow cytometer to obtain EGFP-expressing cells, and amplifying a monoclonal cell line; and identifying a positive p73 reporter gene cell line through PCR identification and Western blot, among screened EGFP-expressing cell lines. The colorectal cancer cell line p73 gene and the EGFP are co-expressed, and the expression level of the EGFP is highly consistent with that of the p73 gene. Therefore, the expression level of the p73 gene can be accurately determined by detecting changes in the expression level of the EGFP. The method for establishing the cell line in the present invention is simple, easy to implement, high in efficiency and precise in gene site positioning.

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