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1. 发明申请

US20160360757A1 Antifouling Composition and Process for Production Thereof 审中-公开
标题翻译：防污组合物及其生产工艺
公开(公告)号：US20160360757A1
公开(公告)日：2016-12-15
申请号：US15178054
申请日：2016-06-09
申请人： Gonçalo Costa , Patrick Freire , Romana Santos , Ana Cristina Silva , Inês Guinote
发明人： Gonçalo Costa , Patrick Freire , Romana Santos , Ana Cristina Silva , Inês Guinote
IPC分类号： A01N63/02 , C12N9/48 , C12N9/52 , C12N1/20
CPC分类号： A01N63/02 , C12N1/02 , C12N1/20 , C12N9/485 , C12N9/52 , C12Y304/00
摘要： This invention concerns a method for preparing a bacterial supernatant comprising culturing a cell of Pseudomonas environmental strain PF-11; and recovering the supernatant. This invention also concerns a method for reducing the amount of a biofilm on a surface, reducing adhesion of at least one organism to a surface, or reducing microfouling or macrofouling on a surface comprising contacting the surface with a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of Pseudomonas strain PF-11; or a composition comprising a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of Pseudomonas strain PF-11, and one or more acceptable carriers. This invention also concerns a method for killing or reducing the growth of a fungus or bacterial cell, or killing or inhibiting the development of an insect or marine copepod, comprising contacting the fungus, bacteria, insect or marine copepod with a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of a Pseudomonas strain PF-11 culture; or a composition comprising a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of a Pseudomonas strain PF-11 culture, and one or more acceptable carriers. This invention also concerns a substantially pure culture of Pseudomonas strain PF-11. This invention also concerns a culture that is enriched in Pseudomonas strain PF-11. This invention also provides a method of identifying whether a bacteria is capable of producing one or more extracellular proteases capable of digesting a high molecular weight substrate.
摘要翻译：本发明涉及一种制备细菌上清液的方法，包括培养环状菌株PF-11的细胞; 并回收上清液。本发明还涉及一种用于减少表面上的生物膜的量的方法，减少至少一种生物体对表面的粘附，或减少表面上的微污染或大量污染，包括使该表面与上清液，上清液级分，修饰的上清液或 Pseudomonas菌株PF-11的修饰上清液部分; 或包含上清液，上清液级分，修饰的上清液或假单胞菌属菌株PF-11的修饰的上清液部分以及一种或多种可接受的载体的组合物。本发明还涉及杀死或减少真菌或细菌细胞生长或杀死或抑制昆虫或海洋桡足类发育的方法，包括使真菌，细菌，昆虫或海洋桡足类与上清液，上清液部分，修饰的上清液或修饰的上清液部分的假单胞菌属菌株PF-11培养物; 或包含上清液，上清液级分，修饰的上清液或假单胞菌属菌株PF-11培养物的修饰的上清液部分以及一种或多种可接受的载体的组合物。本发明还涉及基本上纯的假单胞菌菌株PF-11的培养物。本发明还涉及富含假单胞菌菌株PF-11的培养物。本发明还提供了鉴定细菌是否能够产生能够消化高分子量底物的一种或多种细胞外蛋白酶的方法。

2. 发明公开

US20240010968A1 Antifouling Composition and Process for Production Thereof 审中-公开
公开(公告)号：US20240010968A1
公开(公告)日：2024-01-11
申请号：US18471077
申请日：2023-09-20
申请人： Gonçalo Costa , Patrick Freire , Romana Santos , Ana Cristina Silva , Inês Guinote
发明人： Gonçalo Costa , Patrick Freire , Romana Santos , Ana Cristina Silva , Inês Guinote
IPC分类号： C12N1/02 , A01N63/27 , C12N1/20 , C12N9/48 , C12N9/52
CPC分类号： C12N1/02 , A01N63/27 , C12N1/20 , C12N9/485 , C12N9/52 , C12Y304/00
摘要： This invention concerns a method for preparing a bacterial supernatant comprising culturing a cell of Pseudomonas environmental strain PF-11; and recovering the supernatant. This invention also concerns a method for reducing the amount of a biofilm on a surface, reducing adhesion of at least one organism to a surface, or reducing microfouling or macrofouling on a surface comprising contacting the surface with a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of Pseudomonas strain PF-11; or a composition comprising a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of Pseudomonas strain PF-11, and one or more acceptable carriers. This invention also concerns a method for killing or reducing the growth of a fungus or bacterial cell, or killing or inhibiting the development of an insect or marine copepod, comprising contacting the fungus, bacteria, insect or marine copepod with a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of a Pseudomonas strain PF-11 culture; or a composition comprising a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of a Pseudomonas strain PF-11 culture, and one or more acceptable carriers. This invention also concerns a substantially pure culture of Pseudomonas strain PF-11. This invention also concerns a culture that is enriched in Pseudomonas strain PF-11. This invention also provides a method of identifying whether a bacteria is capable of producing one or more extracellular proteases capable of digesting a high molecular weight substrate.

3. 发明申请

US20220106556A1 Antifouling Composition and Process for Production Thereof 有权
公开(公告)号：US20220106556A1
公开(公告)日：2022-04-07
申请号：US17554399
申请日：2021-12-17
申请人： Gonçalo Costa , Patrick Freire , Romana Santos , Ana Cristina Silva , Inês Guinote
发明人： Gonçalo Costa , Patrick Freire , Romana Santos , Ana Cristina Silva , Inês Guinote
IPC分类号： C12N1/02 , A01N63/27 , C12N1/20 , C12N9/48 , C12N9/52
摘要： This invention concerns a method for preparing a bacterial supernatant comprising culturing a cell of Pseudomonas environmental strain PF-11; and recovering the supernatant. This invention also concerns a method for reducing the amount of a biofilm on a surface, reducing adhesion of at least one organism to a surface, or reducing microfouling or macrofouling on a surface comprising contacting the surface with a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of Pseudomonas strain PF-11; or a composition comprising a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of Pseudomonas strain PF-11, and one or more acceptable carriers. This invention also concerns a method for killing or reducing the growth of a fungus or bacterial cell, or killing or inhibiting the development of an insect or marine copepod, comprising contacting the fungus, bacteria, insect or marine copepod with a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of a Pseudomonas strain PF-11 culture; or a composition comprising a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of a Pseudomonas strain PF-11 culture, and one or more acceptable carriers. This invention also concerns a substantially pure culture of Pseudomonas strain PF-11. This invention also concerns a culture that is enriched in Pseudomonas strain PF-11. This invention also provides a method of identifying whether a bacteria is capable of producing one or more extracellular proteases capable of digesting a high molecular weight substrate.

4. 发明公开

US20230203427A1 Antifouling Composition and Process for Production Thereof 审中-公开
公开(公告)号：US20230203427A1
公开(公告)日：2023-06-29
申请号：US18171136
申请日：2023-02-17
申请人： Gonçalo Costa , Patrick Freire , Romana Santos , Ana Cristina Silva , Inês Guinote
发明人： Gonçalo Costa , Patrick Freire , Romana Santos , Ana Cristina Silva , Inês Guinote
IPC分类号： C12N1/02 , A01N63/27 , C12N1/20 , C12N9/48 , C12N9/52
CPC分类号： C12N1/02 , A01N63/27 , C12N1/20 , C12N9/52 , C12N9/485 , C12Y304/00
摘要： This invention concerns a method for preparing a bacterial supernatant comprising culturing a cell of Pseudomonas environmental strain PF-11; and recovering the supernatant. This invention also concerns a method for reducing the amount of a biofilm on a surface, reducing adhesion of at least one organism to a surface, or reducing microfouling or macrofouling on a surface comprising contacting the surface with a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of Pseudomonas strain PF-11; or a composition comprising a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of Pseudomonas strain PF-11, and one or more acceptable carriers. This invention also concerns a method for killing or reducing the growth of a fungus or bacterial cell, or killing or inhibiting the development of an insect or marine copepod, comprising contacting the fungus, bacteria, insect or marine copepod with a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of a Pseudomonas strain PF-11 culture; or a composition comprising a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of a Pseudomonas strain PF-11 culture, and one or more acceptable carriers. This invention also concerns a substantially pure culture of Pseudomonas strain PF-11. This invention also concerns a culture that is enriched in Pseudomonas strain PF-11. This invention also provides a method of identifying whether a bacteria is capable of producing one or more extracellular proteases capable of digesting a high molecular weight substrate.

5. 发明公开

US20240209311A1 Antifouling Composition and Process for Production Thereof 审中-公开
公开(公告)号：US20240209311A1
公开(公告)日：2024-06-27
申请号：US18432964
申请日：2024-02-05
申请人： Gonçalo Costa , Patrick Freire , Romana Santos , Ana Cristina Silva , Inês Guinote
发明人： Gonçalo Costa , Patrick Freire , Romana Santos , Ana Cristina Silva , Inês Guinote
IPC分类号： C12N1/02 , A01N63/27 , C12N1/20 , C12N9/48 , C12N9/52
CPC分类号： C12N1/02 , A01N63/27 , C12N1/20 , C12N9/485 , C12N9/52 , C12Y304/00
摘要： This invention concerns a method for preparing a bacterial supernatant comprising culturing a cell of Pseudomonas environmental strain PF-11; and recovering the supernatant. This invention also concerns a method for reducing the amount of a biofilm on a surface, reducing adhesion of at least one organism to a surface, or reducing microfouling or macrofouling on a surface comprising contacting the surface with a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of Pseudomonas strain PF-11; or a composition comprising a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of Pseudomonas strain PF-11, and one or more acceptable carriers. This invention also concerns a method for killing or reducing the growth of a fungus or bacterial cell, or killing or inhibiting the development of an insect or marine copepod, comprising contacting the fungus, bacteria, insect or marine copepod with a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of a Pseudomonas strain PF-11 culture; or a composition comprising a supernatant, supernatant fraction, modified supernatant or modified supernatant fraction of a Pseudomonas strain PF-11 culture, and one or more acceptable carriers. This invention also concerns a substantially pure culture of Pseudomonas strain PF-11. This invention also concerns a culture that is enriched in Pseudomonas strain PF-11. This invention also provides a method of identifying whether a bacteria is capable of producing one or more extracellular proteases capable of digesting a high molecular weight substrate.

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