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1. 发明授权

US5183740A Flow immunosensor method and apparatus 失效
标题翻译：流动免疫传感器方法和装置
公开(公告)号：US5183740A
公开(公告)日：1993-02-02
申请号：US486024
申请日：1990-02-23
申请人： Frances S. Ligler , Gaber Bruce P. , Anne W. Kusterbeck , Gregory A. Wemhoff
发明人： Frances S. Ligler , Gaber Bruce P. , Anne W. Kusterbeck , Gregory A. Wemhoff
IPC分类号： G01N33/536 , G01N33/53 , G01N33/543 , G01N35/02
CPC分类号： G01N33/54366 , G01N33/53 , G01N33/54306 , Y10S435/80 , Y10S435/804 , Y10S435/961 , Y10S435/968
摘要： A method of detecting a target moiety comprising the steps of (a) providing an antibody specific to the target, (b) saturating the binding sites of the antibody with a labelled form of the target, (c) flowing a liquid containing the target past the saturated antibody, thereby (d) allowing the target to displace the labelled antigen, and (e) detecting the displaced labelled antigen with a detector for the label.Also, the invention is accomplished by a flow immunosensor comprising: a liquid flowing through the immunosensor for moving a sample through the immunosensor; a receiving means for introducing the sample to the liquid stream; a flow control means for moving the liquid stream through the immunosensor; an exchanger connected to the sample receiving means in which the sample is brought in contact with labelled antigens of the target and whereby any target present is allowed to displace the labelled antigen; a detection apparatus connected to the exchanger for detecting any labelled antigen which is released; and a disposal means connected to the detection apparatus for disposing of or collecting of waste coming from the detection apparatus.
摘要翻译：一种检测靶部分的方法，其包括以下步骤：（a）提供对靶特异性的抗体，（b）用靶标的标记形式使抗体的结合位点饱和，（c）使包含靶标的液体流过饱和抗体，从而（d）使靶标置换标记的抗原，（e）用标记的检测器检测置换的标记的抗原。此外，本发明通过流动免疫传感器实现，该流动免疫传感器包括：流过免疫传感器的液体，用于使样品移动通过免疫传感器; 用于将样品引入液体流的接收装置; 用于使液体流移动通过免疫传感器的流量控制装置; 连接到样品接收装置的交换器，其中样品与靶标的标记抗原接触，并且由此允许存在的任何靶标置换标记抗原; 连接到交换器的检测装置，用于检测被释放的任何标记的抗原; 以及与检测装置连接的处置装置，用于处置或收集来自检测装置的废物。

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