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1. 发明申请

US20060049051A1 Microfabricated chip and method of use 有权
公开(公告)号：US20060049051A1
公开(公告)日：2006-03-09
申请号：US10934259
申请日：2004-09-03
申请人： Edward Yeung , Ho-Ming Pang , Futian Han
发明人： Edward Yeung , Ho-Ming Pang , Futian Han
IPC分类号： C07K1/26 , G01N27/447
CPC分类号： G01N27/44721 , C07K1/26 , G01N27/44791
摘要： A multiplexed, absorbance-based microfabricated chip electrophoresis system, microfabricated chip, and method of use are included for the detection and identification of protein species. The microfabricated chip electrophoresis system capable of analyzing multiple samples simultaneously. The system uses a microfabricated chip having a sample chamber for first dimension separation of a sample, a first planar array of channels for second dimension separation of the sample approximately perpendicular the sample chamber, and a barrier separating the sample chamber from the first planar array of channels during the first dimension separation. In use, the sample is placed into a sample chamber, a barrier is established around the sample chamber, the sample is isoelectric focused within the sample chamber, the barrier is then removed, and the sample separated in the first array of channels by molecular weight.

2. 发明授权

US07497937B2 Microfabricated chip and method of use 有权
标题翻译：微型芯片及其使用方法
公开(公告)号：US07497937B2
公开(公告)日：2009-03-03
申请号：US10934259
申请日：2004-09-03
申请人： Edward S. Yeung , Ho-Ming Pang , Futian Han
发明人： Edward S. Yeung , Ho-Ming Pang , Futian Han
IPC分类号： G01N27/453
CPC分类号： G01N27/44721 , C07K1/26 , G01N27/44791
摘要： A multiplexed, absorbance-based microfabricated chip electrophoresis system, microfabricated chip, and method of use are included for the detection and identification of protein species. The microfabricated chip electrophoresis system capable of analyzing multiple samples simultaneously. The system uses a microfabricated chip having a sample chamber for first dimension separation of a sample, a first planar array of channels for second dimension separation of the sample approximately perpendicular the sample chamber, and a barrier separating the sample chamber from the first planar array of channels during the first dimension separation. In use, the sample is placed into a sample chamber, a barrier is established around the sample chamber, the sample is isoelectric focused within the sample chamber, the barrier is then removed, and the sample separated in the first array of channels by molecular weight.
摘要翻译：包含复合的，以吸光度为基础的微芯片电泳系统，微加工芯片和使用方法用于检测和鉴定蛋白质。该微电解芯片电泳系统能够同时分析多个样品。该系统使用具有样品室的微制造芯片，用于样品的第一维分离，用于样品大致垂直于样品室的第二维分离的通道的第一平面阵列，以及将样品室与第一平面阵列通道在第一维分离期间。在使用中，将样品放置在样品室中，在样品室周围建立屏障，样品在样品室内等电聚焦，然后除去屏障，样品在第一阵列通道中以分子量分离。

3. 发明授权

US06969452B2 Two-dimensional protein separations using chromatofocusing and multiplexed capillary gel electrophoresis 有权
标题翻译：使用色谱聚焦和多重毛细管凝胶电泳的二维蛋白质分离
公开(公告)号：US06969452B2
公开(公告)日：2005-11-29
申请号：US10377909
申请日：2003-02-28
申请人： Yan He , Ho-Ming Pang , Siquan Luo , Futian Han
发明人： Yan He , Ho-Ming Pang , Siquan Luo , Futian Han
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44773
摘要： Disclosed is a two-dimensional protein separation method. It makes separating a protein sample by chromatofocusing into a plurality of aliquots, and then loading each aliquot into a separate capillary tube; and separating each aliquot by multiplexed capillary electrophoresis to produce a two-dimensional array of separated proteins. A preferred integrated buffer for this system is also disclosed.
摘要翻译：公开了二维蛋白质分离方法。它使蛋白质样品通过色谱聚焦分成多个等分试样，然后将每个等分试样装载到单独的毛细管中; 并通过多重毛细管电泳分离每个等分试样以产生分离的蛋白质的二维阵列。还公开了该系统的优选集成缓冲器。

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